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ICP-MS trace element analysis in serum and whole blood

Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential detection of the trace elements (K, Zn, Se, Cu,...

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Autores principales: Laur, Nico, Kinscherf, Ralf, Pomytkin, Karolina, Kaiser, Lars, Knes, Otto, Deigner, Hans-Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239469/
https://www.ncbi.nlm.nih.gov/pubmed/32433650
http://dx.doi.org/10.1371/journal.pone.0233357
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author Laur, Nico
Kinscherf, Ralf
Pomytkin, Karolina
Kaiser, Lars
Knes, Otto
Deigner, Hans-Peter
author_facet Laur, Nico
Kinscherf, Ralf
Pomytkin, Karolina
Kaiser, Lars
Knes, Otto
Deigner, Hans-Peter
author_sort Laur, Nico
collection PubMed
description Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential detection of the trace elements (K, Zn, Se, Cu, Mn, Fe, Mg) in serum and whole blood by an ICP-MS method using single work-up, which is a simple, quick and robust method for the sequential measurement and quantification of the trace elements Sodium (Na), Potassium (K), Calcium (Ca), Zinc (Zn), Selenium (Se), Copper (Cu), Iron (Fe), Manganese (Mn) and Magnesium (Mg) in whole blood as well as Copper (Cu), Selenium (Se), Zinc (Zn), Iron (Fe), Magnesium (Mg), Manganese (Mn), Chromium (Cr), Nickel (Ni), Gold (Au) and Lithium (Li) in human serum. For analysis, only 100 μl of serum or whole blood is sufficient, which make this method suitable for detecting trace element deficiency or excess in newborns and infants. All samples were processed and analyzed by ICP-MS (Agilent Technologies). The accuracy, precision, linearity and the limit of quantification (LOQ), Limit of Blank (LOB) and the limit of detection (LOD) of the method were assessed. Recovery rates were between 80–130% for most of the analyzed elements; repeatabilities (C(v) %) calculated were below 15% for most of the measured elements. The validity of the proposed methodology was assessed by analyzing a certified human serum and whole blood material with known concentrations for all elements; the method described is ready for routine use in biomonitoring studies.
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spelling pubmed-72394692020-06-08 ICP-MS trace element analysis in serum and whole blood Laur, Nico Kinscherf, Ralf Pomytkin, Karolina Kaiser, Lars Knes, Otto Deigner, Hans-Peter PLoS One Research Article Trace elements and minerals are compounds that are essential for the support of a variety of biological functions and play an important role in the formation of and the defense against oxidative stress. Here we describe a technique, allowing sequential detection of the trace elements (K, Zn, Se, Cu, Mn, Fe, Mg) in serum and whole blood by an ICP-MS method using single work-up, which is a simple, quick and robust method for the sequential measurement and quantification of the trace elements Sodium (Na), Potassium (K), Calcium (Ca), Zinc (Zn), Selenium (Se), Copper (Cu), Iron (Fe), Manganese (Mn) and Magnesium (Mg) in whole blood as well as Copper (Cu), Selenium (Se), Zinc (Zn), Iron (Fe), Magnesium (Mg), Manganese (Mn), Chromium (Cr), Nickel (Ni), Gold (Au) and Lithium (Li) in human serum. For analysis, only 100 μl of serum or whole blood is sufficient, which make this method suitable for detecting trace element deficiency or excess in newborns and infants. All samples were processed and analyzed by ICP-MS (Agilent Technologies). The accuracy, precision, linearity and the limit of quantification (LOQ), Limit of Blank (LOB) and the limit of detection (LOD) of the method were assessed. Recovery rates were between 80–130% for most of the analyzed elements; repeatabilities (C(v) %) calculated were below 15% for most of the measured elements. The validity of the proposed methodology was assessed by analyzing a certified human serum and whole blood material with known concentrations for all elements; the method described is ready for routine use in biomonitoring studies. Public Library of Science 2020-05-20 /pmc/articles/PMC7239469/ /pubmed/32433650 http://dx.doi.org/10.1371/journal.pone.0233357 Text en © 2020 Laur et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Laur, Nico
Kinscherf, Ralf
Pomytkin, Karolina
Kaiser, Lars
Knes, Otto
Deigner, Hans-Peter
ICP-MS trace element analysis in serum and whole blood
title ICP-MS trace element analysis in serum and whole blood
title_full ICP-MS trace element analysis in serum and whole blood
title_fullStr ICP-MS trace element analysis in serum and whole blood
title_full_unstemmed ICP-MS trace element analysis in serum and whole blood
title_short ICP-MS trace element analysis in serum and whole blood
title_sort icp-ms trace element analysis in serum and whole blood
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239469/
https://www.ncbi.nlm.nih.gov/pubmed/32433650
http://dx.doi.org/10.1371/journal.pone.0233357
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