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Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas
Pediatric high-grade gliomas (HGG) are rare aggressive tumors that present a prognostic and therapeutic challenge. Diffuse midline glioma, H3K27M–mutant is a new entity introduced to HGG in the latest WHO classification. In this study we evaluated the presence of H3K27M mutation in 105 tumor samples...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239884/ https://www.ncbi.nlm.nih.gov/pubmed/32433577 http://dx.doi.org/10.1038/s41598-020-65272-x |
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author | Mosaab, Amal El-Ayadi, Moatasem Khorshed, Eman N. Amer, Nada Refaat, Amal El-Beltagy, Mohamed Hassan, Zeinab Soror, Sameh H. Zaghloul, Mohamed Saad El-Naggar, Shahenda |
author_facet | Mosaab, Amal El-Ayadi, Moatasem Khorshed, Eman N. Amer, Nada Refaat, Amal El-Beltagy, Mohamed Hassan, Zeinab Soror, Sameh H. Zaghloul, Mohamed Saad El-Naggar, Shahenda |
author_sort | Mosaab, Amal |
collection | PubMed |
description | Pediatric high-grade gliomas (HGG) are rare aggressive tumors that present a prognostic and therapeutic challenge. Diffuse midline glioma, H3K27M–mutant is a new entity introduced to HGG in the latest WHO classification. In this study we evaluated the presence of H3K27M mutation in 105 tumor samples histologically classified into low-grade gliomas (LGG) (n = 45), and HGG (n = 60). Samples were screened for the mutation in histone H3.3 and H3.1 variants to examine its prevalence, prognostic impact, and assess its potential clinical value in limited resource settings. H3K27M mutation was detected in 28 of 105 (26.7%) samples, and its distribution was significantly associated with midline locations (p-value < 0.0001) and HGG (p-value = 0.003). Overall and event- free survival (OS and EFS, respectively) of patients with mutant tumors did not differ significantly, neither according to histologic grade (OS p-value = 0.736, EFS p-value = 0.75) nor across anatomical sites (OS p-value = 0.068, EFS p-value = 0.153). Detection of H3K27M mutation in pediatric gliomas provides more precise risk stratification compared to traditional histopathological techniques. Hence, mutation detection should be pursued in all pediatric gliomas. Meanwhile, focusing on midline LGG can be an alternative in lower-middle-income countries to maximally optimize patients’ treatment options. |
format | Online Article Text |
id | pubmed-7239884 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72398842020-05-29 Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas Mosaab, Amal El-Ayadi, Moatasem Khorshed, Eman N. Amer, Nada Refaat, Amal El-Beltagy, Mohamed Hassan, Zeinab Soror, Sameh H. Zaghloul, Mohamed Saad El-Naggar, Shahenda Sci Rep Article Pediatric high-grade gliomas (HGG) are rare aggressive tumors that present a prognostic and therapeutic challenge. Diffuse midline glioma, H3K27M–mutant is a new entity introduced to HGG in the latest WHO classification. In this study we evaluated the presence of H3K27M mutation in 105 tumor samples histologically classified into low-grade gliomas (LGG) (n = 45), and HGG (n = 60). Samples were screened for the mutation in histone H3.3 and H3.1 variants to examine its prevalence, prognostic impact, and assess its potential clinical value in limited resource settings. H3K27M mutation was detected in 28 of 105 (26.7%) samples, and its distribution was significantly associated with midline locations (p-value < 0.0001) and HGG (p-value = 0.003). Overall and event- free survival (OS and EFS, respectively) of patients with mutant tumors did not differ significantly, neither according to histologic grade (OS p-value = 0.736, EFS p-value = 0.75) nor across anatomical sites (OS p-value = 0.068, EFS p-value = 0.153). Detection of H3K27M mutation in pediatric gliomas provides more precise risk stratification compared to traditional histopathological techniques. Hence, mutation detection should be pursued in all pediatric gliomas. Meanwhile, focusing on midline LGG can be an alternative in lower-middle-income countries to maximally optimize patients’ treatment options. Nature Publishing Group UK 2020-05-20 /pmc/articles/PMC7239884/ /pubmed/32433577 http://dx.doi.org/10.1038/s41598-020-65272-x Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Mosaab, Amal El-Ayadi, Moatasem Khorshed, Eman N. Amer, Nada Refaat, Amal El-Beltagy, Mohamed Hassan, Zeinab Soror, Sameh H. Zaghloul, Mohamed Saad El-Naggar, Shahenda Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title | Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title_full | Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title_fullStr | Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title_full_unstemmed | Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title_short | Histone H3K27M Mutation Overrides Histological Grading in Pediatric Gliomas |
title_sort | histone h3k27m mutation overrides histological grading in pediatric gliomas |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7239884/ https://www.ncbi.nlm.nih.gov/pubmed/32433577 http://dx.doi.org/10.1038/s41598-020-65272-x |
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