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ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5

BACKGROUND: Epithelial-Mesenchymal Transition (EMT) is a major process in the initiation of tumor metastasis, where cancer cells lose sessile epithelial potential and gain mesenchymal phenotype. Large-scale cell identity shifts are often orchestrated on an epigenetic level and the interplay between...

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Autores principales: Shen, Chaoqin, Yan, Tingting, Tong, Tianying, Shi, Debin, Ren, Linlin, Zhang, Youwei, Zhang, Xinyu, Cao, Yingying, Yan, Yuqing, Ma, Yanru, Zhu, Xiaoqiang, Tian, Xianglong, Fang, Jing-Yuan, Chen, Haoyan, Ji, Linhua, Hong, Jie, Xuan, Baoqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240015/
https://www.ncbi.nlm.nih.gov/pubmed/32478065
http://dx.doi.org/10.3389/fcell.2020.00293
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author Shen, Chaoqin
Yan, Tingting
Tong, Tianying
Shi, Debin
Ren, Linlin
Zhang, Youwei
Zhang, Xinyu
Cao, Yingying
Yan, Yuqing
Ma, Yanru
Zhu, Xiaoqiang
Tian, Xianglong
Fang, Jing-Yuan
Chen, Haoyan
Ji, Linhua
Hong, Jie
Xuan, Baoqin
author_facet Shen, Chaoqin
Yan, Tingting
Tong, Tianying
Shi, Debin
Ren, Linlin
Zhang, Youwei
Zhang, Xinyu
Cao, Yingying
Yan, Yuqing
Ma, Yanru
Zhu, Xiaoqiang
Tian, Xianglong
Fang, Jing-Yuan
Chen, Haoyan
Ji, Linhua
Hong, Jie
Xuan, Baoqin
author_sort Shen, Chaoqin
collection PubMed
description BACKGROUND: Epithelial-Mesenchymal Transition (EMT) is a major process in the initiation of tumor metastasis, where cancer cells lose sessile epithelial potential and gain mesenchymal phenotype. Large-scale cell identity shifts are often orchestrated on an epigenetic level and the interplay between epigenetic factors and EMT progression was still largely unknown. In this study, we tried to identify candidate epigenetic factors that involved in EMT progression. METHODS: Colorectal cancer (CRC) cells were transfected with an arrayed shRNA library targeting 384 genes involved in epigenetic modification. Candidate genes were identified by real-time PCR. Western blot, RNA-seq and gene set enrichment analysis were conducted to confirm the suppressive role of ALKBH4 in EMT. The clinical relevance of ALKBH4 in CRC was investigated in two independent Renji Cohorts and a microarray dataset (GSE21510) from GEO database. In vitro transwell assay and in vivo metastatic tumor model were performed to explore the biological function of ALKBH4 in the metastasis of CRC. Co-IP (Co-Immunoprecipitation) and ChIP (Chromatin Immunoprecipitation) assays were employed to uncover the mechanism. RESULTS: We screened for candidate epigenetic factors that affected EMT process and identified ALKBH4 as a candidate EMT suppressor gene, which was significantly downregulated in CRC patients. Decreased level of ALKBH4 was associated with metastasis and predicted poor prognosis of CRC patients. Follow-up functional experiments illustrated overexpression of ALKBH4 inhibited the invasion ability of CRC cells in vitro, as well as their metastatic capability in vivo. Mechanistically, CO-IP and ChIP assays indicated that ALKBH4 competitively bound WDR5 (a key component of histone methyltransferase complex) and decreased H3K4me3 histone modification on the target genes including MIR21. CONCLUSIONS: This study illustrated that ALKBH4 may function as a novel metastasis suppressor of CRC, and inhibits H3K4me3 modification through binding WDR5 during EMT.
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spelling pubmed-72400152020-05-29 ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5 Shen, Chaoqin Yan, Tingting Tong, Tianying Shi, Debin Ren, Linlin Zhang, Youwei Zhang, Xinyu Cao, Yingying Yan, Yuqing Ma, Yanru Zhu, Xiaoqiang Tian, Xianglong Fang, Jing-Yuan Chen, Haoyan Ji, Linhua Hong, Jie Xuan, Baoqin Front Cell Dev Biol Cell and Developmental Biology BACKGROUND: Epithelial-Mesenchymal Transition (EMT) is a major process in the initiation of tumor metastasis, where cancer cells lose sessile epithelial potential and gain mesenchymal phenotype. Large-scale cell identity shifts are often orchestrated on an epigenetic level and the interplay between epigenetic factors and EMT progression was still largely unknown. In this study, we tried to identify candidate epigenetic factors that involved in EMT progression. METHODS: Colorectal cancer (CRC) cells were transfected with an arrayed shRNA library targeting 384 genes involved in epigenetic modification. Candidate genes were identified by real-time PCR. Western blot, RNA-seq and gene set enrichment analysis were conducted to confirm the suppressive role of ALKBH4 in EMT. The clinical relevance of ALKBH4 in CRC was investigated in two independent Renji Cohorts and a microarray dataset (GSE21510) from GEO database. In vitro transwell assay and in vivo metastatic tumor model were performed to explore the biological function of ALKBH4 in the metastasis of CRC. Co-IP (Co-Immunoprecipitation) and ChIP (Chromatin Immunoprecipitation) assays were employed to uncover the mechanism. RESULTS: We screened for candidate epigenetic factors that affected EMT process and identified ALKBH4 as a candidate EMT suppressor gene, which was significantly downregulated in CRC patients. Decreased level of ALKBH4 was associated with metastasis and predicted poor prognosis of CRC patients. Follow-up functional experiments illustrated overexpression of ALKBH4 inhibited the invasion ability of CRC cells in vitro, as well as their metastatic capability in vivo. Mechanistically, CO-IP and ChIP assays indicated that ALKBH4 competitively bound WDR5 (a key component of histone methyltransferase complex) and decreased H3K4me3 histone modification on the target genes including MIR21. CONCLUSIONS: This study illustrated that ALKBH4 may function as a novel metastasis suppressor of CRC, and inhibits H3K4me3 modification through binding WDR5 during EMT. Frontiers Media S.A. 2020-05-14 /pmc/articles/PMC7240015/ /pubmed/32478065 http://dx.doi.org/10.3389/fcell.2020.00293 Text en Copyright © 2020 Shen, Yan, Tong, Shi, Ren, Zhang, Zhang, Cao, Yan, Ma, Zhu, Tian, Fang, Chen, Ji, Hong and Xuan. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Shen, Chaoqin
Yan, Tingting
Tong, Tianying
Shi, Debin
Ren, Linlin
Zhang, Youwei
Zhang, Xinyu
Cao, Yingying
Yan, Yuqing
Ma, Yanru
Zhu, Xiaoqiang
Tian, Xianglong
Fang, Jing-Yuan
Chen, Haoyan
Ji, Linhua
Hong, Jie
Xuan, Baoqin
ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title_full ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title_fullStr ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title_full_unstemmed ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title_short ALKBH4 Functions as a Suppressor of Colorectal Cancer Metastasis via Competitively Binding to WDR5
title_sort alkbh4 functions as a suppressor of colorectal cancer metastasis via competitively binding to wdr5
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240015/
https://www.ncbi.nlm.nih.gov/pubmed/32478065
http://dx.doi.org/10.3389/fcell.2020.00293
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