Cargando…

Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers

Aegilops caudata L. [syn. Ae. markgrafii (Greuter) Hammer], is a diploid wild relative of wheat (2n = 2x = 14, CC) and a valuable source for new genetic diversity for wheat improvement. It has a variety of disease resistance factors along with tolerance for various abiotic stresses and can be used f...

Descripción completa

Detalles Bibliográficos
Autores principales: Grewal, Surbhi, Othmeni, Manel, Walker, Jack, Hubbart-Edwards, Stella, Yang, Cai-yun, Scholefield, Duncan, Ashling, Stephen, Isaac, Peter, King, Ian P., King, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240103/
https://www.ncbi.nlm.nih.gov/pubmed/32477394
http://dx.doi.org/10.3389/fpls.2020.00606
_version_ 1783536811380834304
author Grewal, Surbhi
Othmeni, Manel
Walker, Jack
Hubbart-Edwards, Stella
Yang, Cai-yun
Scholefield, Duncan
Ashling, Stephen
Isaac, Peter
King, Ian P.
King, Julie
author_facet Grewal, Surbhi
Othmeni, Manel
Walker, Jack
Hubbart-Edwards, Stella
Yang, Cai-yun
Scholefield, Duncan
Ashling, Stephen
Isaac, Peter
King, Ian P.
King, Julie
author_sort Grewal, Surbhi
collection PubMed
description Aegilops caudata L. [syn. Ae. markgrafii (Greuter) Hammer], is a diploid wild relative of wheat (2n = 2x = 14, CC) and a valuable source for new genetic diversity for wheat improvement. It has a variety of disease resistance factors along with tolerance for various abiotic stresses and can be used for wheat improvement through the generation of genome-wide introgressions resulting in different wheat–Ae. caudata recombinant lines. Here, we report the generation of nine such wheat–Ae. caudata recombinant lines which were characterized using wheat genome-specific KASP (Kompetitive Allele Specific PCR) markers and multi-color genomic in situ hybridization (mcGISH). Of these, six lines have stable homozygous introgressions from Ae. caudata and will be used for future trait analysis. Using cytological techniques and molecular marker analysis of the recombinant lines, 182 KASP markers were physically mapped onto the seven Ae. caudata chromosomes, of which 155 were polymorphic specifically with only one wheat subgenome. Comparative analysis of the physical positions of these markers in the Ae. caudata and wheat genomes confirmed that the former had chromosomal rearrangements with respect to wheat, as previously reported. These wheat–Ae. caudata recombinant lines and KASP markers are useful resources that can be used in breeding programs worldwide for wheat improvement. Additionally, the genome-specific KASP markers could prove to be a valuable tool for the rapid detection and marker-assisted selection of other Aegilops species in a wheat background.
format Online
Article
Text
id pubmed-7240103
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-72401032020-05-29 Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers Grewal, Surbhi Othmeni, Manel Walker, Jack Hubbart-Edwards, Stella Yang, Cai-yun Scholefield, Duncan Ashling, Stephen Isaac, Peter King, Ian P. King, Julie Front Plant Sci Plant Science Aegilops caudata L. [syn. Ae. markgrafii (Greuter) Hammer], is a diploid wild relative of wheat (2n = 2x = 14, CC) and a valuable source for new genetic diversity for wheat improvement. It has a variety of disease resistance factors along with tolerance for various abiotic stresses and can be used for wheat improvement through the generation of genome-wide introgressions resulting in different wheat–Ae. caudata recombinant lines. Here, we report the generation of nine such wheat–Ae. caudata recombinant lines which were characterized using wheat genome-specific KASP (Kompetitive Allele Specific PCR) markers and multi-color genomic in situ hybridization (mcGISH). Of these, six lines have stable homozygous introgressions from Ae. caudata and will be used for future trait analysis. Using cytological techniques and molecular marker analysis of the recombinant lines, 182 KASP markers were physically mapped onto the seven Ae. caudata chromosomes, of which 155 were polymorphic specifically with only one wheat subgenome. Comparative analysis of the physical positions of these markers in the Ae. caudata and wheat genomes confirmed that the former had chromosomal rearrangements with respect to wheat, as previously reported. These wheat–Ae. caudata recombinant lines and KASP markers are useful resources that can be used in breeding programs worldwide for wheat improvement. Additionally, the genome-specific KASP markers could prove to be a valuable tool for the rapid detection and marker-assisted selection of other Aegilops species in a wheat background. Frontiers Media S.A. 2020-05-14 /pmc/articles/PMC7240103/ /pubmed/32477394 http://dx.doi.org/10.3389/fpls.2020.00606 Text en Copyright © 2020 Grewal, Othmeni, Walker, Hubbart-Edwards, Yang, Scholefield, Ashling, Isaac, King and King. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Grewal, Surbhi
Othmeni, Manel
Walker, Jack
Hubbart-Edwards, Stella
Yang, Cai-yun
Scholefield, Duncan
Ashling, Stephen
Isaac, Peter
King, Ian P.
King, Julie
Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title_full Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title_fullStr Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title_full_unstemmed Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title_short Development of Wheat-Aegilops caudata Introgression Lines and Their Characterization Using Genome-Specific KASP Markers
title_sort development of wheat-aegilops caudata introgression lines and their characterization using genome-specific kasp markers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240103/
https://www.ncbi.nlm.nih.gov/pubmed/32477394
http://dx.doi.org/10.3389/fpls.2020.00606
work_keys_str_mv AT grewalsurbhi developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT othmenimanel developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT walkerjack developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT hubbartedwardsstella developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT yangcaiyun developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT scholefieldduncan developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT ashlingstephen developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT isaacpeter developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT kingianp developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers
AT kingjulie developmentofwheataegilopscaudataintrogressionlinesandtheircharacterizationusinggenomespecifickaspmarkers