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RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis

Perilipin1 (PLIN1), the most abundant lipid droplet (LD)-associated protein, plays a vital role in regulating lipid storage and breakdown in adipocytes. Recently, we found that the overexpression of PLIN1 promotes chicken preadipocyte lipid accumulation. However, the mechanisms by which transcriptio...

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Autores principales: Sun, Yuhang, Zhai, Guiying, Li, Rui, Zhou, Weinan, Li, Yumao, Cao, Zhiping, Wang, Ning, Li, Hui, Wang, Yuxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240111/
https://www.ncbi.nlm.nih.gov/pubmed/32478078
http://dx.doi.org/10.3389/fcell.2020.00349
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author Sun, Yuhang
Zhai, Guiying
Li, Rui
Zhou, Weinan
Li, Yumao
Cao, Zhiping
Wang, Ning
Li, Hui
Wang, Yuxiang
author_facet Sun, Yuhang
Zhai, Guiying
Li, Rui
Zhou, Weinan
Li, Yumao
Cao, Zhiping
Wang, Ning
Li, Hui
Wang, Yuxiang
author_sort Sun, Yuhang
collection PubMed
description Perilipin1 (PLIN1), the most abundant lipid droplet (LD)-associated protein, plays a vital role in regulating lipid storage and breakdown in adipocytes. Recently, we found that the overexpression of PLIN1 promotes chicken preadipocyte lipid accumulation. However, the mechanisms by which transcription of the chicken PLIN1 gene is regulated remain unknown. In this study, we investigated the role of retinoid X receptor α (RXRα) in transcription of the chicken PLIN1 gene. Notably, reporter gene and expression assays showed that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner. Furthermore, promoter deletion and electrophoretic mobility shift assay (EMSA) analysis revealed that the chicken PLIN1 gene promoter region (-774/-785) contains an RXRα-binding site. Further study demonstrated that RXRα overexpression promotes differentiation of an immortalized chicken preadipocyte cell line (ICP1), causing a concomitant increase in PLIN1 transcripts. Taken together, our results show for the first time that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner, which might be at least in part responsible for RXRα-induced adipogenesis.
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spelling pubmed-72401112020-05-29 RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis Sun, Yuhang Zhai, Guiying Li, Rui Zhou, Weinan Li, Yumao Cao, Zhiping Wang, Ning Li, Hui Wang, Yuxiang Front Cell Dev Biol Cell and Developmental Biology Perilipin1 (PLIN1), the most abundant lipid droplet (LD)-associated protein, plays a vital role in regulating lipid storage and breakdown in adipocytes. Recently, we found that the overexpression of PLIN1 promotes chicken preadipocyte lipid accumulation. However, the mechanisms by which transcription of the chicken PLIN1 gene is regulated remain unknown. In this study, we investigated the role of retinoid X receptor α (RXRα) in transcription of the chicken PLIN1 gene. Notably, reporter gene and expression assays showed that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner. Furthermore, promoter deletion and electrophoretic mobility shift assay (EMSA) analysis revealed that the chicken PLIN1 gene promoter region (-774/-785) contains an RXRα-binding site. Further study demonstrated that RXRα overexpression promotes differentiation of an immortalized chicken preadipocyte cell line (ICP1), causing a concomitant increase in PLIN1 transcripts. Taken together, our results show for the first time that RXRα activates transcription of the chicken PLIN1 gene in a PPARγ-independent manner, which might be at least in part responsible for RXRα-induced adipogenesis. Frontiers Media S.A. 2020-05-14 /pmc/articles/PMC7240111/ /pubmed/32478078 http://dx.doi.org/10.3389/fcell.2020.00349 Text en Copyright © 2020 Sun, Zhai, Li, Zhou, Li, Cao, Wang, Li and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Sun, Yuhang
Zhai, Guiying
Li, Rui
Zhou, Weinan
Li, Yumao
Cao, Zhiping
Wang, Ning
Li, Hui
Wang, Yuxiang
RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title_full RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title_fullStr RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title_full_unstemmed RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title_short RXRα Positively Regulates Expression of the Chicken PLIN1 Gene in a PPARγ-Independent Manner and Promotes Adipogenesis
title_sort rxrα positively regulates expression of the chicken plin1 gene in a pparγ-independent manner and promotes adipogenesis
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240111/
https://www.ncbi.nlm.nih.gov/pubmed/32478078
http://dx.doi.org/10.3389/fcell.2020.00349
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