Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression

Objective: The present study is designed to evaluate the anti-tumor effects of myrrh on human gastric cancer both in vitro and in vivo. Methods: The gastric cancer cell proliferation was determined by MTT assay. Apoptosis was measured by flow cytometry and Hoechst 33342 staining. Wound healing was p...

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Autores principales: Sun, Mengxue, Hua, Jie, Liu, Gaoshuang, Huang, Peiyun, Liu, Ningsheng, He, Xiaopu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2020
Materias:
Cell Death & Injury
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240199/
https://www.ncbi.nlm.nih.gov/pubmed/32364228
http://dx.doi.org/10.1042/BSR20192372
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author Sun, Mengxue
Hua, Jie
Liu, Gaoshuang
Huang, Peiyun
Liu, Ningsheng
He, Xiaopu
author_facet Sun, Mengxue
Hua, Jie
Liu, Gaoshuang
Huang, Peiyun
Liu, Ningsheng
He, Xiaopu
author_sort Sun, Mengxue
collection PubMed
description Objective: The present study is designed to evaluate the anti-tumor effects of myrrh on human gastric cancer both in vitro and in vivo. Methods: The gastric cancer cell proliferation was determined by MTT assay. Apoptosis was measured by flow cytometry and Hoechst 33342 staining. Wound healing was performed to evaluate the effects of myrrh on the migration. COX-2, PCNA, Bcl-2, and Bax expressions were detected by Western blot analysis. A xenograft nude mice model of human gastric cancer was established to evaluate the anti-cancer effect of myrrh in vivo. Results: Myrrh significantly inhibited cellular proliferation, migration, and induced apoptosis in vitro as well as inhibited tumor growth in vivo. In addition, myrrh inhibited the expression of PCNA, COX-2, and Bcl-2 as well as increased Bax expression in gastric cancer cells. Conclusion: Myrrh may inhibit the proliferation and migration of gastric cancer cells, as well as induced their apoptosis by down-regulating the expression of COX-2.
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spelling pubmed-72401992020-06-04 Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression Sun, Mengxue Hua, Jie Liu, Gaoshuang Huang, Peiyun Liu, Ningsheng He, Xiaopu Biosci Rep Cell Death & Injury Objective: The present study is designed to evaluate the anti-tumor effects of myrrh on human gastric cancer both in vitro and in vivo. Methods: The gastric cancer cell proliferation was determined by MTT assay. Apoptosis was measured by flow cytometry and Hoechst 33342 staining. Wound healing was performed to evaluate the effects of myrrh on the migration. COX-2, PCNA, Bcl-2, and Bax expressions were detected by Western blot analysis. A xenograft nude mice model of human gastric cancer was established to evaluate the anti-cancer effect of myrrh in vivo. Results: Myrrh significantly inhibited cellular proliferation, migration, and induced apoptosis in vitro as well as inhibited tumor growth in vivo. In addition, myrrh inhibited the expression of PCNA, COX-2, and Bcl-2 as well as increased Bax expression in gastric cancer cells. Conclusion: Myrrh may inhibit the proliferation and migration of gastric cancer cells, as well as induced their apoptosis by down-regulating the expression of COX-2. Portland Press Ltd. 2020-05-20 /pmc/articles/PMC7240199/ /pubmed/32364228 http://dx.doi.org/10.1042/BSR20192372 Text en © 2020 The Author(s). https://creativecommons.org/licenses/by/4.0/ This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY).
spellingShingle Cell Death & Injury
Sun, Mengxue
Hua, Jie
Liu, Gaoshuang
Huang, Peiyun
Liu, Ningsheng
He, Xiaopu
Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title_full Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title_fullStr Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title_full_unstemmed Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title_short Myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
title_sort myrrh induces the apoptosis and inhibits the proliferation and migration of gastric cancer cells through down-regulating cyclooxygenase-2 expression
topic Cell Death & Injury
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240199/
https://www.ncbi.nlm.nih.gov/pubmed/32364228
http://dx.doi.org/10.1042/BSR20192372
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