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Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19
Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinforma...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V.
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240266/ https://www.ncbi.nlm.nih.gov/pubmed/32445875 http://dx.doi.org/10.1016/j.jviromet.2020.113888 |
| _version_ | 1783536846215577600 |
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| author | Campos, Gubio S. Sardi, Silvia I. Falcao, Melissa B. Belitardo, Emilia M.M.A. Rocha, Danilo J.P.G. Rolo, Carolina A. Menezes, Aline D. Pinheiro, Carina S. Carvalho, Rejane H. Almeida, João P.P. Aguiar, Eric R.G.R. Pacheco, Luis G.C. |
| author_facet | Campos, Gubio S. Sardi, Silvia I. Falcao, Melissa B. Belitardo, Emilia M.M.A. Rocha, Danilo J.P.G. Rolo, Carolina A. Menezes, Aline D. Pinheiro, Carina S. Carvalho, Rejane H. Almeida, João P.P. Aguiar, Eric R.G.R. Pacheco, Luis G.C. |
| author_sort | Campos, Gubio S. |
| collection | PubMed |
| description | Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. |
| format | Online Article Text |
| id | pubmed-7240266 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | Elsevier B.V. |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-72402662020-05-21 Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 Campos, Gubio S. Sardi, Silvia I. Falcao, Melissa B. Belitardo, Emilia M.M.A. Rocha, Danilo J.P.G. Rolo, Carolina A. Menezes, Aline D. Pinheiro, Carina S. Carvalho, Rejane H. Almeida, João P.P. Aguiar, Eric R.G.R. Pacheco, Luis G.C. J Virol Methods Article Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus. Elsevier B.V. 2020-08 2020-05-21 /pmc/articles/PMC7240266/ /pubmed/32445875 http://dx.doi.org/10.1016/j.jviromet.2020.113888 Text en © 2020 Elsevier B.V. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
| spellingShingle | Article Campos, Gubio S. Sardi, Silvia I. Falcao, Melissa B. Belitardo, Emilia M.M.A. Rocha, Danilo J.P.G. Rolo, Carolina A. Menezes, Aline D. Pinheiro, Carina S. Carvalho, Rejane H. Almeida, João P.P. Aguiar, Eric R.G.R. Pacheco, Luis G.C. Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title | Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title_full | Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title_fullStr | Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title_full_unstemmed | Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title_short | Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19 |
| title_sort | ion torrent-based nasopharyngeal swab metatranscriptomics in covid-19 |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240266/ https://www.ncbi.nlm.nih.gov/pubmed/32445875 http://dx.doi.org/10.1016/j.jviromet.2020.113888 |
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