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Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration

Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of...

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Autores principales: Sartika, Dewi, Wang, Chih-Hsin, Wang, Ding-Han, Cherng, Juin-Hong, Chang, Shu-Jen, Fan, Gang-Yi, Wang, Yi-Wen, Lee, Chian-Her, Hong, Po-Da, Wang, Chih-Chien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240549/
https://www.ncbi.nlm.nih.gov/pubmed/32272682
http://dx.doi.org/10.3390/polym12040853
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author Sartika, Dewi
Wang, Chih-Hsin
Wang, Ding-Han
Cherng, Juin-Hong
Chang, Shu-Jen
Fan, Gang-Yi
Wang, Yi-Wen
Lee, Chian-Her
Hong, Po-Da
Wang, Chih-Chien
author_facet Sartika, Dewi
Wang, Chih-Hsin
Wang, Ding-Han
Cherng, Juin-Hong
Chang, Shu-Jen
Fan, Gang-Yi
Wang, Yi-Wen
Lee, Chian-Her
Hong, Po-Da
Wang, Chih-Chien
author_sort Sartika, Dewi
collection PubMed
description Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications.
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spelling pubmed-72405492020-06-11 Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration Sartika, Dewi Wang, Chih-Hsin Wang, Ding-Han Cherng, Juin-Hong Chang, Shu-Jen Fan, Gang-Yi Wang, Yi-Wen Lee, Chian-Her Hong, Po-Da Wang, Chih-Chien Polymers (Basel) Article Recently, stem cell-based bone tissue engineering (BTE) has been recognized as a preferable and clinically significant strategy for bone repair. In this study, a pure 3D silk fibroin (SF) scaffold was fabricated as a BTE material using a lyophilization method. We aimed to investigate the efficacy of the SF scaffold with and without seeded human adipose-derived mesenchymal stem cells (hASCs) in facilitating bone regeneration. The effectiveness of the SF-hASCs scaffold was evaluated based on physical characterization, biocompatibility, osteogenic differentiation in vitro, and bone regeneration in critical rat calvarial defects in vivo. The SF scaffold demonstrated superior biocompatibility and significantly promoted osteogenic differentiation of hASCs in vitro. At six and twelve weeks postimplantation, micro-CT showed no statistical difference in new bone formation amongst all groups. However, histological staining results revealed that the SF-hASCs scaffold exhibited a better bone extracellular matrix deposition in the defect regions compared to other groups. Immunohistochemical staining confirmed this result; expression of osteoblast-related genes (BMP-2, COL1a1, and OCN) with the SF-hASCs scaffold treatment was remarkably positive, indicating their ability to achieve effective bone remodeling. Thus, these findings demonstrate that SF can serve as a potential carrier for stem cells, to be used as an osteoconductive bioscaffold for BTE applications. MDPI 2020-04-07 /pmc/articles/PMC7240549/ /pubmed/32272682 http://dx.doi.org/10.3390/polym12040853 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sartika, Dewi
Wang, Chih-Hsin
Wang, Ding-Han
Cherng, Juin-Hong
Chang, Shu-Jen
Fan, Gang-Yi
Wang, Yi-Wen
Lee, Chian-Her
Hong, Po-Da
Wang, Chih-Chien
Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title_full Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title_fullStr Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title_full_unstemmed Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title_short Human Adipose-Derived Mesenchymal Stem Cells-Incorporated Silk Fibroin as a Potential Bio-Scaffold in Guiding Bone Regeneration
title_sort human adipose-derived mesenchymal stem cells-incorporated silk fibroin as a potential bio-scaffold in guiding bone regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240549/
https://www.ncbi.nlm.nih.gov/pubmed/32272682
http://dx.doi.org/10.3390/polym12040853
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