Cargando…

Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)

The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBP...

Descripción completa

Detalles Bibliográficos
Autores principales: Pang, Kun, Dong, Shengzhang, Hao, Peiying, Chen, Tongtong, Wang, Xinlong, Yu, Xiaoping, Lin, Huafeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240585/
https://www.ncbi.nlm.nih.gov/pubmed/32230918
http://dx.doi.org/10.3390/insects11040209
_version_ 1783536917634088960
author Pang, Kun
Dong, Shengzhang
Hao, Peiying
Chen, Tongtong
Wang, Xinlong
Yu, Xiaoping
Lin, Huafeng
author_facet Pang, Kun
Dong, Shengzhang
Hao, Peiying
Chen, Tongtong
Wang, Xinlong
Yu, Xiaoping
Lin, Huafeng
author_sort Pang, Kun
collection PubMed
description The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH.
format Online
Article
Text
id pubmed-7240585
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72405852020-06-11 Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae) Pang, Kun Dong, Shengzhang Hao, Peiying Chen, Tongtong Wang, Xinlong Yu, Xiaoping Lin, Huafeng Insects Article The white-backed planthopper (WBPH) Sogatella furcifera is one of the most harmful pests of rice in Southeast Asia. The fat body of WBPH harbors intracellular yeast-like symbionts (YLS). YLS are vertically transmitted to WBPH offspring by transovarial infection. YLS play an important role in the WBPH life cycle. YLS diversity and function have been extensively studied in the brown planthopper (BPH) and small brown planthopper but not in WBPH, even though a novel strategy for controlling the BPH based on suppressing YLS has been proposed. Here, using denaturing gradient gel electrophoresis, we identified 12 unique fungal sequences among YLS of WBPH, and five of them represented uncultured fungi. We then fed WBPH with rice plants treated with different fungicides [70% propineb wettable powder (WP) (PR), 70% propamocarb hydrochloride aqueous solution (AS) (PH), 25% trifloxystrobin and 50% tebuconazole water-dispersible granules (WG) (TT), 40% pyrimethanil suspension concentrate (SC) (PY), and 50% iprodione SC (IP)] and evaluated their effects on YLS abundance and WBPH survival rate. Both YLS abundance and adult WBPH survival rate were significantly decreased upon feeding fungicide-treated rice plants, and exposure to 50% IP resulted in the strongest reduction. The abundance of two Sf-YLS species (Ascomycetes symbiotes and Cla-like symbiotes) was significantly reduced upon exposure to 50% IP. The counts of Ascomycetes symbiotes, the most abundant YLS species, were also suppressed by the other fungicides tested. In conclusion, 50% IP was the most effective fungicide, reducing YLS abundance and WBPH survival rate under controlled conditions, suggesting its potential use to control WBPH. MDPI 2020-03-27 /pmc/articles/PMC7240585/ /pubmed/32230918 http://dx.doi.org/10.3390/insects11040209 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pang, Kun
Dong, Shengzhang
Hao, Peiying
Chen, Tongtong
Wang, Xinlong
Yu, Xiaoping
Lin, Huafeng
Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title_full Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title_fullStr Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title_full_unstemmed Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title_short Fungicides Reduce the Abundance of Yeast-like Symbionts and Survival of White-Backed Planthopper Sogatella furcifera (Homoptera: Delphacidae)
title_sort fungicides reduce the abundance of yeast-like symbionts and survival of white-backed planthopper sogatella furcifera (homoptera: delphacidae)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240585/
https://www.ncbi.nlm.nih.gov/pubmed/32230918
http://dx.doi.org/10.3390/insects11040209
work_keys_str_mv AT pangkun fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT dongshengzhang fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT haopeiying fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT chentongtong fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT wangxinlong fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT yuxiaoping fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae
AT linhuafeng fungicidesreducetheabundanceofyeastlikesymbiontsandsurvivalofwhitebackedplanthoppersogatellafurciferahomopteradelphacidae