Cargando…
Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates
A miscibility study between oppositely charged polyelectrolytes, namely hyaluronic acid and a lactose-modified chitosan, is here reported. Experimental variables such as polymers’ weight ratios, pH values, ionic strengths and hyaluronic acid molecular weights were considered. Transmittance analyses...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240600/ https://www.ncbi.nlm.nih.gov/pubmed/32294992 http://dx.doi.org/10.3390/polym12040897 |
_version_ | 1783536921457197056 |
---|---|
author | Vecchies, Federica Sacco, Pasquale Marsich, Eleonora Cinelli, Giuseppe Lopez, Francesco Donati, Ivan |
author_facet | Vecchies, Federica Sacco, Pasquale Marsich, Eleonora Cinelli, Giuseppe Lopez, Francesco Donati, Ivan |
author_sort | Vecchies, Federica |
collection | PubMed |
description | A miscibility study between oppositely charged polyelectrolytes, namely hyaluronic acid and a lactose-modified chitosan, is here reported. Experimental variables such as polymers’ weight ratios, pH values, ionic strengths and hyaluronic acid molecular weights were considered. Transmittance analyses demonstrated the mutual solubility of the two biopolymers at a neutral pH. The onset of the liquid-liquid phase separation due to electrostatic interactions between the two polymers was detected at pH 4.5, and it was found to be affected by the overall ionic strength, the modality of mixing and the polymers’ weight ratio. Thorough Dynamic Light Scattering (DLS) measurements were performed to check the quality of the formed coacervates by investigating their dimensions, homogeneity and surface charge. The whole DLS results highlighted the influence of the hyaluronic acid molecular weight in affecting coacervates’ dispersity and size. |
format | Online Article Text |
id | pubmed-7240600 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72406002020-06-11 Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates Vecchies, Federica Sacco, Pasquale Marsich, Eleonora Cinelli, Giuseppe Lopez, Francesco Donati, Ivan Polymers (Basel) Article A miscibility study between oppositely charged polyelectrolytes, namely hyaluronic acid and a lactose-modified chitosan, is here reported. Experimental variables such as polymers’ weight ratios, pH values, ionic strengths and hyaluronic acid molecular weights were considered. Transmittance analyses demonstrated the mutual solubility of the two biopolymers at a neutral pH. The onset of the liquid-liquid phase separation due to electrostatic interactions between the two polymers was detected at pH 4.5, and it was found to be affected by the overall ionic strength, the modality of mixing and the polymers’ weight ratio. Thorough Dynamic Light Scattering (DLS) measurements were performed to check the quality of the formed coacervates by investigating their dimensions, homogeneity and surface charge. The whole DLS results highlighted the influence of the hyaluronic acid molecular weight in affecting coacervates’ dispersity and size. MDPI 2020-04-13 /pmc/articles/PMC7240600/ /pubmed/32294992 http://dx.doi.org/10.3390/polym12040897 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Vecchies, Federica Sacco, Pasquale Marsich, Eleonora Cinelli, Giuseppe Lopez, Francesco Donati, Ivan Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title | Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title_full | Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title_fullStr | Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title_full_unstemmed | Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title_short | Binary Solutions of Hyaluronan and Lactose-Modified Chitosan: The Influence of Experimental Variables in Assembling Complex Coacervates |
title_sort | binary solutions of hyaluronan and lactose-modified chitosan: the influence of experimental variables in assembling complex coacervates |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240600/ https://www.ncbi.nlm.nih.gov/pubmed/32294992 http://dx.doi.org/10.3390/polym12040897 |
work_keys_str_mv | AT vecchiesfederica binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates AT saccopasquale binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates AT marsicheleonora binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates AT cinelligiuseppe binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates AT lopezfrancesco binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates AT donatiivan binarysolutionsofhyaluronanandlactosemodifiedchitosantheinfluenceofexperimentalvariablesinassemblingcomplexcoacervates |