Cargando…

Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan

The policy regarding mosquito control strategies in Taiwan is based on integrated vector management (IVM). The major approach is source reduction via collaboration by both residents and governments. However, small and cryptic habitats of dengue vectors are hard to find and eliminate in urban communi...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Ying-An, Lai, Yi-Ting, Wu, Kuo-Chih, Yen, Tsai-Ying, Chen, Chia-Yang, Tsai, Kun-Hsien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240724/
https://www.ncbi.nlm.nih.gov/pubmed/32316283
http://dx.doi.org/10.3390/insects11040251
_version_ 1783536946777161728
author Chen, Ying-An
Lai, Yi-Ting
Wu, Kuo-Chih
Yen, Tsai-Ying
Chen, Chia-Yang
Tsai, Kun-Hsien
author_facet Chen, Ying-An
Lai, Yi-Ting
Wu, Kuo-Chih
Yen, Tsai-Ying
Chen, Chia-Yang
Tsai, Kun-Hsien
author_sort Chen, Ying-An
collection PubMed
description The policy regarding mosquito control strategies in Taiwan is based on integrated vector management (IVM). The major approach is source reduction via collaboration by both residents and governments. However, small and cryptic habitats of dengue vectors are hard to find and eliminate in urban communities. Therefore, this study evaluated a complementary approach that targeted cryptic habitats by utilizing mosquitoes themselves as vehicles to transfer an insect growth regulator, pyriproxyfen (PPF), to their breeding sites; the amount of PPF in breeding water was determined with ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC–MS/MS). A bioassay conducted by introducing ten late-instar larvae into PPF solution was performed to assess emergence inhibition (EI). PPF was found at 0.56 ± 0.04 ng in 25 mL of water by dissemination via ten Aedes aegypti mosquitoes exposed to 0.01% PPF, leading to 100% EI. After the community-level source reduction, a field trial in Kaohsiung in Southern Taiwan showed that 30.8–31.5% of cryptic ovitraps reached EI ≥ 50% one month after spraying 0.01% PPF in microhabitats favored by mosquitoes. IVM in parallel with residual spraying of PPF on resting surfaces of mosquitoes could serve as a simple and complementary approach to reduce cryptic larval sources in urban communities in Southern Taiwan.
format Online
Article
Text
id pubmed-7240724
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72407242020-06-11 Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan Chen, Ying-An Lai, Yi-Ting Wu, Kuo-Chih Yen, Tsai-Ying Chen, Chia-Yang Tsai, Kun-Hsien Insects Article The policy regarding mosquito control strategies in Taiwan is based on integrated vector management (IVM). The major approach is source reduction via collaboration by both residents and governments. However, small and cryptic habitats of dengue vectors are hard to find and eliminate in urban communities. Therefore, this study evaluated a complementary approach that targeted cryptic habitats by utilizing mosquitoes themselves as vehicles to transfer an insect growth regulator, pyriproxyfen (PPF), to their breeding sites; the amount of PPF in breeding water was determined with ultra-performance liquid chromatography coupled with tandem mass spectrometry (UPLC–MS/MS). A bioassay conducted by introducing ten late-instar larvae into PPF solution was performed to assess emergence inhibition (EI). PPF was found at 0.56 ± 0.04 ng in 25 mL of water by dissemination via ten Aedes aegypti mosquitoes exposed to 0.01% PPF, leading to 100% EI. After the community-level source reduction, a field trial in Kaohsiung in Southern Taiwan showed that 30.8–31.5% of cryptic ovitraps reached EI ≥ 50% one month after spraying 0.01% PPF in microhabitats favored by mosquitoes. IVM in parallel with residual spraying of PPF on resting surfaces of mosquitoes could serve as a simple and complementary approach to reduce cryptic larval sources in urban communities in Southern Taiwan. MDPI 2020-04-16 /pmc/articles/PMC7240724/ /pubmed/32316283 http://dx.doi.org/10.3390/insects11040251 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Ying-An
Lai, Yi-Ting
Wu, Kuo-Chih
Yen, Tsai-Ying
Chen, Chia-Yang
Tsai, Kun-Hsien
Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title_full Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title_fullStr Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title_full_unstemmed Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title_short Using UPLC–MS/MS to Evaluate the Dissemination of Pyriproxyfen by Aedes Mosquitoes to Combat Cryptic Larval Habitats after Source Reduction in Kaohsiung in Southern Taiwan
title_sort using uplc–ms/ms to evaluate the dissemination of pyriproxyfen by aedes mosquitoes to combat cryptic larval habitats after source reduction in kaohsiung in southern taiwan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240724/
https://www.ncbi.nlm.nih.gov/pubmed/32316283
http://dx.doi.org/10.3390/insects11040251
work_keys_str_mv AT chenyingan usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan
AT laiyiting usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan
AT wukuochih usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan
AT yentsaiying usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan
AT chenchiayang usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan
AT tsaikunhsien usinguplcmsmstoevaluatethedisseminationofpyriproxyfenbyaedesmosquitoestocombatcrypticlarvalhabitatsaftersourcereductioninkaohsiunginsoutherntaiwan