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Dual-Layered Nanomaterial-Based Molecular Pattering on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule, Anandamide Neurotransmitter
[Image: see text] In this endeavor, a novel electrochemical biosensor was designed using multiwall carbon nanotubes (MWCNTs)- and nickel nanoparticles (NiNPs)-embedded anandamide (AEA) imprinted polymer. The NiNPs so synthesized were mortared with MWCNTs and molecularly imprinted polymer (MIP), whic...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240810/ https://www.ncbi.nlm.nih.gov/pubmed/32455194 http://dx.doi.org/10.1021/acsomega.0c00285 |
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author | Jain, Utkarsh Soni, Shringika Balhara, Yatan Pal Singh Khanuja, Manika Chauhan, Nidhi |
author_facet | Jain, Utkarsh Soni, Shringika Balhara, Yatan Pal Singh Khanuja, Manika Chauhan, Nidhi |
author_sort | Jain, Utkarsh |
collection | PubMed |
description | [Image: see text] In this endeavor, a novel electrochemical biosensor was designed using multiwall carbon nanotubes (MWCNTs)- and nickel nanoparticles (NiNPs)-embedded anandamide (AEA) imprinted polymer. The NiNPs so synthesized were mortared with MWCNTs and molecularly imprinted polymer (MIP), which enhanced sensitivity and selectivity of the developed sensor, respectively. The characterization methods of AEA-based MIP included X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and Brunauer–Emmett–Teller (BET) analysis, which supported the successful synthesis of the polymer. Electrochemical studies of fabricated sensor were performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy in potentiostatic mode (PEIS). In this first phase of AEA-specific sensor development, MWCNT/NiNP/MIP@SPE was found to successfully discriminate between different concentrations of AEA. The developed sensing platform demonstrated a 100 pM–1 nM linear range with a 0.01 nM detection limit (LOD), 0.0149 mA/pM sensitivity, and 50% stability within 4 months. The sensor demonstrated selectivity toward AEA: although acetylcholine (ACh) and dopamine acted as strong interfering components because of their chemical similarity, the spiked AEA samples demonstrated ∼90% recoveries. Hence, our results have passed the first step in AEA detection at home, although with a clinical setup, future advancement is still required. |
format | Online Article Text |
id | pubmed-7240810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-72408102020-05-22 Dual-Layered Nanomaterial-Based Molecular Pattering on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule, Anandamide Neurotransmitter Jain, Utkarsh Soni, Shringika Balhara, Yatan Pal Singh Khanuja, Manika Chauhan, Nidhi ACS Omega [Image: see text] In this endeavor, a novel electrochemical biosensor was designed using multiwall carbon nanotubes (MWCNTs)- and nickel nanoparticles (NiNPs)-embedded anandamide (AEA) imprinted polymer. The NiNPs so synthesized were mortared with MWCNTs and molecularly imprinted polymer (MIP), which enhanced sensitivity and selectivity of the developed sensor, respectively. The characterization methods of AEA-based MIP included X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), and Brunauer–Emmett–Teller (BET) analysis, which supported the successful synthesis of the polymer. Electrochemical studies of fabricated sensor were performed using cyclic voltammetry (CV) and electrochemical impedance spectroscopy in potentiostatic mode (PEIS). In this first phase of AEA-specific sensor development, MWCNT/NiNP/MIP@SPE was found to successfully discriminate between different concentrations of AEA. The developed sensing platform demonstrated a 100 pM–1 nM linear range with a 0.01 nM detection limit (LOD), 0.0149 mA/pM sensitivity, and 50% stability within 4 months. The sensor demonstrated selectivity toward AEA: although acetylcholine (ACh) and dopamine acted as strong interfering components because of their chemical similarity, the spiked AEA samples demonstrated ∼90% recoveries. Hence, our results have passed the first step in AEA detection at home, although with a clinical setup, future advancement is still required. American Chemical Society 2020-05-07 /pmc/articles/PMC7240810/ /pubmed/32455194 http://dx.doi.org/10.1021/acsomega.0c00285 Text en Copyright © 2020 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes. |
spellingShingle | Jain, Utkarsh Soni, Shringika Balhara, Yatan Pal Singh Khanuja, Manika Chauhan, Nidhi Dual-Layered Nanomaterial-Based Molecular Pattering on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule, Anandamide Neurotransmitter |
title | Dual-Layered Nanomaterial-Based Molecular Pattering
on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule,
Anandamide Neurotransmitter |
title_full | Dual-Layered Nanomaterial-Based Molecular Pattering
on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule,
Anandamide Neurotransmitter |
title_fullStr | Dual-Layered Nanomaterial-Based Molecular Pattering
on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule,
Anandamide Neurotransmitter |
title_full_unstemmed | Dual-Layered Nanomaterial-Based Molecular Pattering
on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule,
Anandamide Neurotransmitter |
title_short | Dual-Layered Nanomaterial-Based Molecular Pattering
on Polymer Surface Biomimetic Impedimetric Sensing of a Bliss Molecule,
Anandamide Neurotransmitter |
title_sort | dual-layered nanomaterial-based molecular pattering
on polymer surface biomimetic impedimetric sensing of a bliss molecule,
anandamide neurotransmitter |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7240810/ https://www.ncbi.nlm.nih.gov/pubmed/32455194 http://dx.doi.org/10.1021/acsomega.0c00285 |
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