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Enzyme Assembly for Compartmentalized Metabolic Flux Control

Enzyme assembly by ligand binding or physically sequestrating enzymes, substrates, or metabolites into isolated compartments can bring key molecules closer to enhance the flux of a metabolic pathway. The emergence of enzyme assembly has provided both opportunities and challenges for metabolic engine...

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Detalles Bibliográficos
Autores principales: Lv, Xueqin, Cui, Shixiu, Gu, Yang, Li, Jianghua, Du, Guocheng, Liu, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241084/
https://www.ncbi.nlm.nih.gov/pubmed/32224973
http://dx.doi.org/10.3390/metabo10040125
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author Lv, Xueqin
Cui, Shixiu
Gu, Yang
Li, Jianghua
Du, Guocheng
Liu, Long
author_facet Lv, Xueqin
Cui, Shixiu
Gu, Yang
Li, Jianghua
Du, Guocheng
Liu, Long
author_sort Lv, Xueqin
collection PubMed
description Enzyme assembly by ligand binding or physically sequestrating enzymes, substrates, or metabolites into isolated compartments can bring key molecules closer to enhance the flux of a metabolic pathway. The emergence of enzyme assembly has provided both opportunities and challenges for metabolic engineering. At present, with the development of synthetic biology and systems biology, a variety of enzyme assembly strategies have been proposed, from the initial direct enzyme fusion to scaffold-free assembly, as well as artificial scaffolds, such as nucleic acid/protein scaffolds, and even some more complex physical compartments. These assembly strategies have been explored and applied to the synthesis of various important bio-based products, and have achieved different degrees of success. Despite some achievements, enzyme assembly, especially in vivo, still has many problems that have attracted significant attention from researchers. Here, we focus on some selected examples to review recent research on scaffold-free strategies, synthetic artificial scaffolds, and physical compartments for enzyme assembly or pathway sequestration, and we discuss their notable advances. In addition, the potential applications and challenges in the applications are highlighted.
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spelling pubmed-72410842020-06-02 Enzyme Assembly for Compartmentalized Metabolic Flux Control Lv, Xueqin Cui, Shixiu Gu, Yang Li, Jianghua Du, Guocheng Liu, Long Metabolites Review Enzyme assembly by ligand binding or physically sequestrating enzymes, substrates, or metabolites into isolated compartments can bring key molecules closer to enhance the flux of a metabolic pathway. The emergence of enzyme assembly has provided both opportunities and challenges for metabolic engineering. At present, with the development of synthetic biology and systems biology, a variety of enzyme assembly strategies have been proposed, from the initial direct enzyme fusion to scaffold-free assembly, as well as artificial scaffolds, such as nucleic acid/protein scaffolds, and even some more complex physical compartments. These assembly strategies have been explored and applied to the synthesis of various important bio-based products, and have achieved different degrees of success. Despite some achievements, enzyme assembly, especially in vivo, still has many problems that have attracted significant attention from researchers. Here, we focus on some selected examples to review recent research on scaffold-free strategies, synthetic artificial scaffolds, and physical compartments for enzyme assembly or pathway sequestration, and we discuss their notable advances. In addition, the potential applications and challenges in the applications are highlighted. MDPI 2020-03-26 /pmc/articles/PMC7241084/ /pubmed/32224973 http://dx.doi.org/10.3390/metabo10040125 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lv, Xueqin
Cui, Shixiu
Gu, Yang
Li, Jianghua
Du, Guocheng
Liu, Long
Enzyme Assembly for Compartmentalized Metabolic Flux Control
title Enzyme Assembly for Compartmentalized Metabolic Flux Control
title_full Enzyme Assembly for Compartmentalized Metabolic Flux Control
title_fullStr Enzyme Assembly for Compartmentalized Metabolic Flux Control
title_full_unstemmed Enzyme Assembly for Compartmentalized Metabolic Flux Control
title_short Enzyme Assembly for Compartmentalized Metabolic Flux Control
title_sort enzyme assembly for compartmentalized metabolic flux control
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241084/
https://www.ncbi.nlm.nih.gov/pubmed/32224973
http://dx.doi.org/10.3390/metabo10040125
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