Cargando…

Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery

Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitr...

Descripción completa

Detalles Bibliográficos
Autores principales: Mani, Ramalingam, Shobha, P. S., Thilagavathi, Saravanan, Prema, Padmanabhan, Viswanathan, Natarajan, Vineet, Ratra, Dhanashree, Ratra, Angayarkanni, Narayanasamy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241722/
https://www.ncbi.nlm.nih.gov/pubmed/32437405
http://dx.doi.org/10.1371/journal.pone.0233517
_version_ 1783537115819147264
author Mani, Ramalingam
Shobha, P. S.
Thilagavathi, Saravanan
Prema, Padmanabhan
Viswanathan, Natarajan
Vineet, Ratra
Dhanashree, Ratra
Angayarkanni, Narayanasamy
author_facet Mani, Ramalingam
Shobha, P. S.
Thilagavathi, Saravanan
Prema, Padmanabhan
Viswanathan, Natarajan
Vineet, Ratra
Dhanashree, Ratra
Angayarkanni, Narayanasamy
author_sort Mani, Ramalingam
collection PubMed
description Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitrectomy surgery (n = 44, mean age: 56.09±10.2 years) were recruited. Dry eye evaluation was done before and 8 weeks after surgery (2 weeks after omitting topical eye drops). Conjunctival imprint cytology for goblet cell count and tear Mucin 5AC (MUC5AC) protein estimation was done. Gene expressions of MUC5AC, MUC4, MUC16, Aquaporin 4 (AQP4) and AQP5 were analyzed in the conjunctival imprint cells by qPCR. None of the patients exhibited clinical signs of dry eye after VR surgery. But the conjunctival goblet cell density (GCD) was significantly lowered post-VR surgery (63% cases, **p = 0.012) with no alterations in the tear MUC5AC protein. Post-VR surgery, the conjunctival cell gene expression of MUC4, MUC16 and AQP4 were significantly increased (*p = 0.025, *p = 0.05 and *p = 0.02 respectively) and AQP5 was significantly lowered (*p = 0.037), with no change in MUC5AC expression. Tear cytokines were significantly increased post-VR surgery (anti-inflammatory: IL1RA, IL4, IL5, IL9, FGF; PDGFbb and pro-inflammatory: IL2, IL6, IL15, GMCSF and IFNg). Though clinical signs of dry eye were not observed after VR surgery, ocular surface changes in the form of reduced GCD, altered MUC5AC, MUC4, MUC16, AQP4, AQP5 and cytokines are suggestive of dry eye outcome at the molecular level especially inpatients aged above 51 years, especially female gender and those who are diabetic.
format Online
Article
Text
id pubmed-7241722
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-72417222020-06-08 Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery Mani, Ramalingam Shobha, P. S. Thilagavathi, Saravanan Prema, Padmanabhan Viswanathan, Natarajan Vineet, Ratra Dhanashree, Ratra Angayarkanni, Narayanasamy PLoS One Research Article Vitreo-retinal (VR) surgeries induce conjunctival changes. However, there are no study reports regarding prevalence and severity of dry eye after these surgeries. This study evaluated dry eye outcome after VR surgery. Patients undergoing VR surgery classified as scleral buckle and microincision vitrectomy surgery (n = 44, mean age: 56.09±10.2 years) were recruited. Dry eye evaluation was done before and 8 weeks after surgery (2 weeks after omitting topical eye drops). Conjunctival imprint cytology for goblet cell count and tear Mucin 5AC (MUC5AC) protein estimation was done. Gene expressions of MUC5AC, MUC4, MUC16, Aquaporin 4 (AQP4) and AQP5 were analyzed in the conjunctival imprint cells by qPCR. None of the patients exhibited clinical signs of dry eye after VR surgery. But the conjunctival goblet cell density (GCD) was significantly lowered post-VR surgery (63% cases, **p = 0.012) with no alterations in the tear MUC5AC protein. Post-VR surgery, the conjunctival cell gene expression of MUC4, MUC16 and AQP4 were significantly increased (*p = 0.025, *p = 0.05 and *p = 0.02 respectively) and AQP5 was significantly lowered (*p = 0.037), with no change in MUC5AC expression. Tear cytokines were significantly increased post-VR surgery (anti-inflammatory: IL1RA, IL4, IL5, IL9, FGF; PDGFbb and pro-inflammatory: IL2, IL6, IL15, GMCSF and IFNg). Though clinical signs of dry eye were not observed after VR surgery, ocular surface changes in the form of reduced GCD, altered MUC5AC, MUC4, MUC16, AQP4, AQP5 and cytokines are suggestive of dry eye outcome at the molecular level especially inpatients aged above 51 years, especially female gender and those who are diabetic. Public Library of Science 2020-05-21 /pmc/articles/PMC7241722/ /pubmed/32437405 http://dx.doi.org/10.1371/journal.pone.0233517 Text en © 2020 Mani et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Mani, Ramalingam
Shobha, P. S.
Thilagavathi, Saravanan
Prema, Padmanabhan
Viswanathan, Natarajan
Vineet, Ratra
Dhanashree, Ratra
Angayarkanni, Narayanasamy
Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title_full Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title_fullStr Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title_full_unstemmed Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title_short Altered mucins and aquaporins indicate dry eye outcome in patients undergoing Vitreo-retinal surgery
title_sort altered mucins and aquaporins indicate dry eye outcome in patients undergoing vitreo-retinal surgery
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241722/
https://www.ncbi.nlm.nih.gov/pubmed/32437405
http://dx.doi.org/10.1371/journal.pone.0233517
work_keys_str_mv AT maniramalingam alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT shobhaps alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT thilagavathisaravanan alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT premapadmanabhan alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT viswanathannatarajan alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT vineetratra alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT dhanashreeratra alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery
AT angayarkanninarayanasamy alteredmucinsandaquaporinsindicatedryeyeoutcomeinpatientsundergoingvitreoretinalsurgery