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The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials

A study was conducted to assess the efficacy of ozone gas in inactivating spores of both Bacillus anthracis and Bacillus subtilis inoculated onto six building materials (glass, wood, carpet, laminate, galvanized metal, and wallboard paper). Testing conditions consisted of ozone gas concentrations ra...

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Detalles Bibliográficos
Autores principales: Wood, Joseph P., Wendling, Morgan, Richter, William, Rogers, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241793/
https://www.ncbi.nlm.nih.gov/pubmed/32437373
http://dx.doi.org/10.1371/journal.pone.0233291
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author Wood, Joseph P.
Wendling, Morgan
Richter, William
Rogers, James
author_facet Wood, Joseph P.
Wendling, Morgan
Richter, William
Rogers, James
author_sort Wood, Joseph P.
collection PubMed
description A study was conducted to assess the efficacy of ozone gas in inactivating spores of both Bacillus anthracis and Bacillus subtilis inoculated onto six building materials (glass, wood, carpet, laminate, galvanized metal, and wallboard paper). Testing conditions consisted of ozone gas concentrations ranging from 7,000–12,000 parts per million (ppm), contact times from 4 to 12 h, and two relative humidity (RH) levels of 75 and 85%. Results showed that increasing the ozone concentration, contact time, and RH generally increased decontamination efficacy. The materials in which the highest decontamination efficacy was achieved for B. anthracis spores were wallboard paper, carpet, and wood with ≥ 6 log(10) reduction (LR) occurring with 9,800 ppm ozone, 85% RH, for 6 h. The laminate and galvanized metal materials were generally more difficult to decontaminate, requiring 12,000 ppm ozone, 85% RH, and 9–12 h contact time to achieve ≥6 LR of B. anthracis. Lastly, overall, there were no significant differences in decontamination efficacy between the two species.
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spelling pubmed-72417932020-06-03 The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials Wood, Joseph P. Wendling, Morgan Richter, William Rogers, James PLoS One Research Article A study was conducted to assess the efficacy of ozone gas in inactivating spores of both Bacillus anthracis and Bacillus subtilis inoculated onto six building materials (glass, wood, carpet, laminate, galvanized metal, and wallboard paper). Testing conditions consisted of ozone gas concentrations ranging from 7,000–12,000 parts per million (ppm), contact times from 4 to 12 h, and two relative humidity (RH) levels of 75 and 85%. Results showed that increasing the ozone concentration, contact time, and RH generally increased decontamination efficacy. The materials in which the highest decontamination efficacy was achieved for B. anthracis spores were wallboard paper, carpet, and wood with ≥ 6 log(10) reduction (LR) occurring with 9,800 ppm ozone, 85% RH, for 6 h. The laminate and galvanized metal materials were generally more difficult to decontaminate, requiring 12,000 ppm ozone, 85% RH, and 9–12 h contact time to achieve ≥6 LR of B. anthracis. Lastly, overall, there were no significant differences in decontamination efficacy between the two species. Public Library of Science 2020-05-21 /pmc/articles/PMC7241793/ /pubmed/32437373 http://dx.doi.org/10.1371/journal.pone.0233291 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Wood, Joseph P.
Wendling, Morgan
Richter, William
Rogers, James
The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title_full The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title_fullStr The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title_full_unstemmed The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title_short The use of ozone gas for the inactivation of Bacillus anthracis and Bacillus subtilis spores on building materials
title_sort use of ozone gas for the inactivation of bacillus anthracis and bacillus subtilis spores on building materials
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241793/
https://www.ncbi.nlm.nih.gov/pubmed/32437373
http://dx.doi.org/10.1371/journal.pone.0233291
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