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Real-time Observation of CRISPR spacer acquisition by Cas1–Cas2 integrase

Cas1 integrase associates with Cas2 to insert short DNA fragments into a CRISPR array, establishing nucleic acid memory in prokaryotes. Here we applied single-molecule FRET methods to the Enterococcus faecalis (Efa) Cas1–Cas2 system to establish a kinetic framework describing target-searching, integ...

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Detalles Bibliográficos
Autores principales:	Budhathoki, Jagat B., Xiao, Yibei, Schuler, Gabriel, Hu, Chunyi, Cheng, Alexander, Ding, Fran, Ke, Ailong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7241818/ https://www.ncbi.nlm.nih.gov/pubmed/32367067 http://dx.doi.org/10.1038/s41594-020-0415-7

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