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Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner

In the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, glucose 6-phosphate dehydrogenase (G6PDH) plays an important role in producing the power for reducing nitrogenase under light conditions. Our previous study showed that thioredoxin suppresses G6PDH by reducing its activator protein OpcA, i...

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Autores principales: Mihara, Shoko, Sugiura, Kazunori, Yoshida, Keisuke, Hisabori, Toru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242069/
https://www.ncbi.nlm.nih.gov/pubmed/31863668
http://dx.doi.org/10.1093/jxb/erz561
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author Mihara, Shoko
Sugiura, Kazunori
Yoshida, Keisuke
Hisabori, Toru
author_facet Mihara, Shoko
Sugiura, Kazunori
Yoshida, Keisuke
Hisabori, Toru
author_sort Mihara, Shoko
collection PubMed
description In the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, glucose 6-phosphate dehydrogenase (G6PDH) plays an important role in producing the power for reducing nitrogenase under light conditions. Our previous study showed that thioredoxin suppresses G6PDH by reducing its activator protein OpcA, implying that G6PDH is inactivated under light conditions because thioredoxins are reduced by the photosynthetic electron transport system in cyanobacteria. To address how Anabaena sp. PCC 7120 maintains G6PDH activity even under light conditions when nitrogen fixation occurs, we investigated the redox regulation system in vegetative cells and specific nitrogen-fixing cells named heterocysts, individually. We found that thioredoxin target proteins were more oxidized in heterocysts than in vegetative cells under light conditions. Alterations in the redox regulation mechanism of heterocysts may affect the redox states of thioredoxin target proteins, including OpcA, so that G6PDH is activated in heterocysts even under light conditions.
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spelling pubmed-72420692020-05-27 Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner Mihara, Shoko Sugiura, Kazunori Yoshida, Keisuke Hisabori, Toru J Exp Bot Research Papers In the nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, glucose 6-phosphate dehydrogenase (G6PDH) plays an important role in producing the power for reducing nitrogenase under light conditions. Our previous study showed that thioredoxin suppresses G6PDH by reducing its activator protein OpcA, implying that G6PDH is inactivated under light conditions because thioredoxins are reduced by the photosynthetic electron transport system in cyanobacteria. To address how Anabaena sp. PCC 7120 maintains G6PDH activity even under light conditions when nitrogen fixation occurs, we investigated the redox regulation system in vegetative cells and specific nitrogen-fixing cells named heterocysts, individually. We found that thioredoxin target proteins were more oxidized in heterocysts than in vegetative cells under light conditions. Alterations in the redox regulation mechanism of heterocysts may affect the redox states of thioredoxin target proteins, including OpcA, so that G6PDH is activated in heterocysts even under light conditions. Oxford University Press 2020-03-25 2019-12-21 /pmc/articles/PMC7242069/ /pubmed/31863668 http://dx.doi.org/10.1093/jxb/erz561 Text en © The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Mihara, Shoko
Sugiura, Kazunori
Yoshida, Keisuke
Hisabori, Toru
Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title_full Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title_fullStr Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title_full_unstemmed Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title_short Thioredoxin targets are regulated in heterocysts of cyanobacterium Anabaena sp. PCC 7120 in a light-independent manner
title_sort thioredoxin targets are regulated in heterocysts of cyanobacterium anabaena sp. pcc 7120 in a light-independent manner
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242069/
https://www.ncbi.nlm.nih.gov/pubmed/31863668
http://dx.doi.org/10.1093/jxb/erz561
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AT yoshidakeisuke thioredoxintargetsareregulatedinheterocystsofcyanobacteriumanabaenasppcc7120inalightindependentmanner
AT hisaboritoru thioredoxintargetsareregulatedinheterocystsofcyanobacteriumanabaenasppcc7120inalightindependentmanner