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Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty

Genes that provide resistance to fungi and/or bacteria usually reduce plant growth and ultimately affect grain yield. Thus, crop breeding programs need to find genetic resources that balance disease resistance with growth. The receptor kinase FERONIA regulates cell growth and survival in Arabidopsis...

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Autores principales: Yang, Zhuhong, Xing, Junjie, Wang, Long, Liu, Yue, Qu, Jianing, Tan, Yang, Fu, Xiqin, Lin, Qinlu, Deng, Huafeng, Yu, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242082/
https://www.ncbi.nlm.nih.gov/pubmed/31986202
http://dx.doi.org/10.1093/jxb/erz541
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author Yang, Zhuhong
Xing, Junjie
Wang, Long
Liu, Yue
Qu, Jianing
Tan, Yang
Fu, Xiqin
Lin, Qinlu
Deng, Huafeng
Yu, Feng
author_facet Yang, Zhuhong
Xing, Junjie
Wang, Long
Liu, Yue
Qu, Jianing
Tan, Yang
Fu, Xiqin
Lin, Qinlu
Deng, Huafeng
Yu, Feng
author_sort Yang, Zhuhong
collection PubMed
description Genes that provide resistance to fungi and/or bacteria usually reduce plant growth and ultimately affect grain yield. Thus, crop breeding programs need to find genetic resources that balance disease resistance with growth. The receptor kinase FERONIA regulates cell growth and survival in Arabidopsis. Here, we investigate, in rice, the role of members of the FERONIA-like receptor (FLR) gene family in the balance between growth and the response to the fungal pathogen Magnaporthe oryzae (Pyricularia oryzae), which causes the most devastating disease in rice. We carried out genome-wide gene expression and functional screenings in rice via a gene knockout strategy, and we successfully knocked out 14 FLR genes in rice. Using these genetic resources, we found that mutations in the FLR2 and FLR11 genes provide resistance to rice blast without a profound growth penalty. Detailed analyses revealed that FLR2 mutation increased both defense-related gene expression and M. oryzae-triggered production of reactive oxygen species. Thus, our results highlight novel genetic tools for studying the underlying molecular mechanisms of enhancing disease resistance without growth penalty.
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spelling pubmed-72420822020-05-27 Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty Yang, Zhuhong Xing, Junjie Wang, Long Liu, Yue Qu, Jianing Tan, Yang Fu, Xiqin Lin, Qinlu Deng, Huafeng Yu, Feng J Exp Bot Research Papers Genes that provide resistance to fungi and/or bacteria usually reduce plant growth and ultimately affect grain yield. Thus, crop breeding programs need to find genetic resources that balance disease resistance with growth. The receptor kinase FERONIA regulates cell growth and survival in Arabidopsis. Here, we investigate, in rice, the role of members of the FERONIA-like receptor (FLR) gene family in the balance between growth and the response to the fungal pathogen Magnaporthe oryzae (Pyricularia oryzae), which causes the most devastating disease in rice. We carried out genome-wide gene expression and functional screenings in rice via a gene knockout strategy, and we successfully knocked out 14 FLR genes in rice. Using these genetic resources, we found that mutations in the FLR2 and FLR11 genes provide resistance to rice blast without a profound growth penalty. Detailed analyses revealed that FLR2 mutation increased both defense-related gene expression and M. oryzae-triggered production of reactive oxygen species. Thus, our results highlight novel genetic tools for studying the underlying molecular mechanisms of enhancing disease resistance without growth penalty. Oxford University Press 2020-03-25 2020-01-27 /pmc/articles/PMC7242082/ /pubmed/31986202 http://dx.doi.org/10.1093/jxb/erz541 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Papers
Yang, Zhuhong
Xing, Junjie
Wang, Long
Liu, Yue
Qu, Jianing
Tan, Yang
Fu, Xiqin
Lin, Qinlu
Deng, Huafeng
Yu, Feng
Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title_full Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title_fullStr Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title_full_unstemmed Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title_short Mutations of two FERONIA-like receptor genes enhance rice blast resistance without growth penalty
title_sort mutations of two feronia-like receptor genes enhance rice blast resistance without growth penalty
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242082/
https://www.ncbi.nlm.nih.gov/pubmed/31986202
http://dx.doi.org/10.1093/jxb/erz541
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