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Hydrodynamic model of directional ciliary-beat organization in human airways

In the lung, the airway surface is protected by mucus, whose transport and evacuation is ensured through active ciliary beating. The mechanisms governing the long-range directional organization of ciliary beats, required for effective mucus transport, are much debated. Here, we experimentally show o...

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Autores principales: Gsell, Simon, Loiseau, Etienne, D’Ortona, Umberto, Viallat, Annie, Favier, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242329/
https://www.ncbi.nlm.nih.gov/pubmed/32439925
http://dx.doi.org/10.1038/s41598-020-64695-w
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author Gsell, Simon
Loiseau, Etienne
D’Ortona, Umberto
Viallat, Annie
Favier, Julien
author_facet Gsell, Simon
Loiseau, Etienne
D’Ortona, Umberto
Viallat, Annie
Favier, Julien
author_sort Gsell, Simon
collection PubMed
description In the lung, the airway surface is protected by mucus, whose transport and evacuation is ensured through active ciliary beating. The mechanisms governing the long-range directional organization of ciliary beats, required for effective mucus transport, are much debated. Here, we experimentally show on human bronchial epithelium reconstituted in-vitro that the dynamics of ciliary-beat orientation is closely connected to hydrodynamic effects. To examine the fundamental mechanisms of this self-organization process, we build a two-dimensional model in which the hydrodynamic coupling between cilia is provided by a streamwise-alignment rule governing the local orientation of the ciliary forcing. The model reproduces the emergence of the mucus swirls observed in the experiments. The predicted swirl sizes, which scale with the ciliary density and mucus viscosity, are in agreement with in-vitro measurements. A transition from the swirly regime to a long-range unidirectional mucus flow allowing effective clearance occurs at high ciliary density and high mucus viscosity. In the latter case, the mucus flow tends to spontaneously align with the bronchus axis due to hydrodynamic effects.
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spelling pubmed-72423292020-05-29 Hydrodynamic model of directional ciliary-beat organization in human airways Gsell, Simon Loiseau, Etienne D’Ortona, Umberto Viallat, Annie Favier, Julien Sci Rep Article In the lung, the airway surface is protected by mucus, whose transport and evacuation is ensured through active ciliary beating. The mechanisms governing the long-range directional organization of ciliary beats, required for effective mucus transport, are much debated. Here, we experimentally show on human bronchial epithelium reconstituted in-vitro that the dynamics of ciliary-beat orientation is closely connected to hydrodynamic effects. To examine the fundamental mechanisms of this self-organization process, we build a two-dimensional model in which the hydrodynamic coupling between cilia is provided by a streamwise-alignment rule governing the local orientation of the ciliary forcing. The model reproduces the emergence of the mucus swirls observed in the experiments. The predicted swirl sizes, which scale with the ciliary density and mucus viscosity, are in agreement with in-vitro measurements. A transition from the swirly regime to a long-range unidirectional mucus flow allowing effective clearance occurs at high ciliary density and high mucus viscosity. In the latter case, the mucus flow tends to spontaneously align with the bronchus axis due to hydrodynamic effects. Nature Publishing Group UK 2020-05-21 /pmc/articles/PMC7242329/ /pubmed/32439925 http://dx.doi.org/10.1038/s41598-020-64695-w Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Gsell, Simon
Loiseau, Etienne
D’Ortona, Umberto
Viallat, Annie
Favier, Julien
Hydrodynamic model of directional ciliary-beat organization in human airways
title Hydrodynamic model of directional ciliary-beat organization in human airways
title_full Hydrodynamic model of directional ciliary-beat organization in human airways
title_fullStr Hydrodynamic model of directional ciliary-beat organization in human airways
title_full_unstemmed Hydrodynamic model of directional ciliary-beat organization in human airways
title_short Hydrodynamic model of directional ciliary-beat organization in human airways
title_sort hydrodynamic model of directional ciliary-beat organization in human airways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242329/
https://www.ncbi.nlm.nih.gov/pubmed/32439925
http://dx.doi.org/10.1038/s41598-020-64695-w
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