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Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice
The main factors involved in the pathogenesis of atopic dermatitis (AD) are skin barrier abnormality, allergy/immunology, and pruritus. Considering how oxidative stress influences these factors, antioxidant agents may be effective candidates in the treatment of AD. To evaluate the effect of Caffeoyl...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242424/ https://www.ncbi.nlm.nih.gov/pubmed/32439906 http://dx.doi.org/10.1038/s41598-020-65502-2 |
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author | Jang, Sunhyae Ohn, Jungyoon Kim, Ji Won Kang, So Min Jeon, Dasom Heo, Chan Yeong Lee, Yoon-Sik Kwon, Ohsang Kim, Kyu Han |
author_facet | Jang, Sunhyae Ohn, Jungyoon Kim, Ji Won Kang, So Min Jeon, Dasom Heo, Chan Yeong Lee, Yoon-Sik Kwon, Ohsang Kim, Kyu Han |
author_sort | Jang, Sunhyae |
collection | PubMed |
description | The main factors involved in the pathogenesis of atopic dermatitis (AD) are skin barrier abnormality, allergy/immunology, and pruritus. Considering how oxidative stress influences these factors, antioxidant agents may be effective candidates in the treatment of AD. To evaluate the effect of Caffeoyl–Pro–His amide (CA-PH), an antioxidant agent, on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like phenotypes in BALB/c mice. Topical sensitization and challenge by DNCB were performed on the dorsal skin of BALB/c mice to induce AD-like cutaneous lesions, phenotypes, and immunologic response. CA-PH was applied topically for 2 weeks to assess its effects on DNCB-induced AD-like phenotypes. As a result, CA-PH relieved DNCB-induced AD-like phenotypes quantified by dermatitis severity score, scratching duration, and trans-epidermal water loss. Histopathological analysis showed that CA-PH decreased epidermal thickening, the number of mast cells, and eosinophil infiltration in dermis. Immunohistochemical staining revealed that CA-PH recovered skin barrier-related proteins: filaggrin, involucrin, and loricrin. As for the immunologic aspects, CA-PH treatment lowered mRNA or protein levels of interleukin (IL)-4, IL-6, IL-17a, IL-1b, IL-31, and IL-33 levels and thymic stromal lymphopoietin (TSLP) levels in cutaneous tissue, reducing the DNCB-induced serum IgE level elevation. In conclusion, topical CA-PH may be a therapeutic option for the treatment of AD. |
format | Online Article Text |
id | pubmed-7242424 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72424242020-05-30 Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice Jang, Sunhyae Ohn, Jungyoon Kim, Ji Won Kang, So Min Jeon, Dasom Heo, Chan Yeong Lee, Yoon-Sik Kwon, Ohsang Kim, Kyu Han Sci Rep Article The main factors involved in the pathogenesis of atopic dermatitis (AD) are skin barrier abnormality, allergy/immunology, and pruritus. Considering how oxidative stress influences these factors, antioxidant agents may be effective candidates in the treatment of AD. To evaluate the effect of Caffeoyl–Pro–His amide (CA-PH), an antioxidant agent, on 2,4-dinitrochlorobenzene (DNCB)-induced AD-like phenotypes in BALB/c mice. Topical sensitization and challenge by DNCB were performed on the dorsal skin of BALB/c mice to induce AD-like cutaneous lesions, phenotypes, and immunologic response. CA-PH was applied topically for 2 weeks to assess its effects on DNCB-induced AD-like phenotypes. As a result, CA-PH relieved DNCB-induced AD-like phenotypes quantified by dermatitis severity score, scratching duration, and trans-epidermal water loss. Histopathological analysis showed that CA-PH decreased epidermal thickening, the number of mast cells, and eosinophil infiltration in dermis. Immunohistochemical staining revealed that CA-PH recovered skin barrier-related proteins: filaggrin, involucrin, and loricrin. As for the immunologic aspects, CA-PH treatment lowered mRNA or protein levels of interleukin (IL)-4, IL-6, IL-17a, IL-1b, IL-31, and IL-33 levels and thymic stromal lymphopoietin (TSLP) levels in cutaneous tissue, reducing the DNCB-induced serum IgE level elevation. In conclusion, topical CA-PH may be a therapeutic option for the treatment of AD. Nature Publishing Group UK 2020-05-21 /pmc/articles/PMC7242424/ /pubmed/32439906 http://dx.doi.org/10.1038/s41598-020-65502-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jang, Sunhyae Ohn, Jungyoon Kim, Ji Won Kang, So Min Jeon, Dasom Heo, Chan Yeong Lee, Yoon-Sik Kwon, Ohsang Kim, Kyu Han Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title | Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title_full | Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title_fullStr | Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title_full_unstemmed | Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title_short | Caffeoyl–Pro–His amide relieve DNCB-Induced Atopic Dermatitis-Like phenotypes in BALB/c mice |
title_sort | caffeoyl–pro–his amide relieve dncb-induced atopic dermatitis-like phenotypes in balb/c mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7242424/ https://www.ncbi.nlm.nih.gov/pubmed/32439906 http://dx.doi.org/10.1038/s41598-020-65502-2 |
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