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DNA vaccine protection against SARS-CoV-2 in rhesus macaques
The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Association for the Advancement of Science
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243363/ https://www.ncbi.nlm.nih.gov/pubmed/32434945 http://dx.doi.org/10.1126/science.abc6284 |
| _version_ | 1783537415925792768 |
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| author | Yu, Jingyou Tostanoski, Lisa H. Peter, Lauren Mercado, Noe B. McMahan, Katherine Mahrokhian, Shant H. Nkolola, Joseph P. Liu, Jinyan Li, Zhenfeng Chandrashekar, Abishek Martinez, David R. Loos, Carolin Atyeo, Caroline Fischinger, Stephanie Burke, John S. Slein, Matthew D. Chen, Yuezhou Zuiani, Adam Lelis, Felipe J. N. Travers, Meghan Habibi, Shaghayegh Pessaint, Laurent Van Ry, Alex Blade, Kelvin Brown, Renita Cook, Anthony Finneyfrock, Brad Dodson, Alan Teow, Elyse Velasco, Jason Zahn, Roland Wegmann, Frank Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. He, Xuan Jacob-Dolan, Catherine Kirilova, Marinela Kordana, Nicole Lin, Zijin Maxfield, Lori F. Nampanya, Felix Nityanandam, Ramya Ventura, John D. Wan, Huahua Cai, Yongfei Chen, Bing Schmidt, Aaron G. Wesemann, Duane R. Baric, Ralph S. Alter, Galit Andersen, Hanne Lewis, Mark G. Barouch, Dan H. |
| author_facet | Yu, Jingyou Tostanoski, Lisa H. Peter, Lauren Mercado, Noe B. McMahan, Katherine Mahrokhian, Shant H. Nkolola, Joseph P. Liu, Jinyan Li, Zhenfeng Chandrashekar, Abishek Martinez, David R. Loos, Carolin Atyeo, Caroline Fischinger, Stephanie Burke, John S. Slein, Matthew D. Chen, Yuezhou Zuiani, Adam Lelis, Felipe J. N. Travers, Meghan Habibi, Shaghayegh Pessaint, Laurent Van Ry, Alex Blade, Kelvin Brown, Renita Cook, Anthony Finneyfrock, Brad Dodson, Alan Teow, Elyse Velasco, Jason Zahn, Roland Wegmann, Frank Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. He, Xuan Jacob-Dolan, Catherine Kirilova, Marinela Kordana, Nicole Lin, Zijin Maxfield, Lori F. Nampanya, Felix Nityanandam, Ramya Ventura, John D. Wan, Huahua Cai, Yongfei Chen, Bing Schmidt, Aaron G. Wesemann, Duane R. Baric, Ralph S. Alter, Galit Andersen, Hanne Lewis, Mark G. Barouch, Dan H. |
| author_sort | Yu, Jingyou |
| collection | PubMed |
| description | The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals developed humoral and cellular immune responses, including neutralizing antibody titers at levels comparable to those found in convalescent humans and macaques infected with SARS-CoV-2. After vaccination, all animals were challenged with SARS-CoV-2, and the vaccine encoding the full-length S protein resulted in >3.1 and >3.7 log(10) reductions in median viral loads in bronchoalveolar lavage and nasal mucosa, respectively, as compared with viral loads in sham controls. Vaccine-elicited neutralizing antibody titers correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate vaccine protection against SARS-CoV-2 in nonhuman primates. |
| format | Online Article Text |
| id | pubmed-7243363 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2020 |
| publisher | American Association for the Advancement of Science |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-72433632020-05-28 DNA vaccine protection against SARS-CoV-2 in rhesus macaques Yu, Jingyou Tostanoski, Lisa H. Peter, Lauren Mercado, Noe B. McMahan, Katherine Mahrokhian, Shant H. Nkolola, Joseph P. Liu, Jinyan Li, Zhenfeng Chandrashekar, Abishek Martinez, David R. Loos, Carolin Atyeo, Caroline Fischinger, Stephanie Burke, John S. Slein, Matthew D. Chen, Yuezhou Zuiani, Adam Lelis, Felipe J. N. Travers, Meghan Habibi, Shaghayegh Pessaint, Laurent Van Ry, Alex Blade, Kelvin Brown, Renita Cook, Anthony Finneyfrock, Brad Dodson, Alan Teow, Elyse Velasco, Jason Zahn, Roland Wegmann, Frank Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. He, Xuan Jacob-Dolan, Catherine Kirilova, Marinela Kordana, Nicole Lin, Zijin Maxfield, Lori F. Nampanya, Felix Nityanandam, Ramya Ventura, John D. Wan, Huahua Cai, Yongfei Chen, Bing Schmidt, Aaron G. Wesemann, Duane R. Baric, Ralph S. Alter, Galit Andersen, Hanne Lewis, Mark G. Barouch, Dan H. Science Research Articles The global coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has made the development of a vaccine a top biomedical priority. In this study, we developed a series of DNA vaccine candidates expressing different forms of the SARS-CoV-2 spike (S) protein and evaluated them in 35 rhesus macaques. Vaccinated animals developed humoral and cellular immune responses, including neutralizing antibody titers at levels comparable to those found in convalescent humans and macaques infected with SARS-CoV-2. After vaccination, all animals were challenged with SARS-CoV-2, and the vaccine encoding the full-length S protein resulted in >3.1 and >3.7 log(10) reductions in median viral loads in bronchoalveolar lavage and nasal mucosa, respectively, as compared with viral loads in sham controls. Vaccine-elicited neutralizing antibody titers correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate vaccine protection against SARS-CoV-2 in nonhuman primates. American Association for the Advancement of Science 2020-08-14 2020-05-20 /pmc/articles/PMC7243363/ /pubmed/32434945 http://dx.doi.org/10.1126/science.abc6284 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Research Articles Yu, Jingyou Tostanoski, Lisa H. Peter, Lauren Mercado, Noe B. McMahan, Katherine Mahrokhian, Shant H. Nkolola, Joseph P. Liu, Jinyan Li, Zhenfeng Chandrashekar, Abishek Martinez, David R. Loos, Carolin Atyeo, Caroline Fischinger, Stephanie Burke, John S. Slein, Matthew D. Chen, Yuezhou Zuiani, Adam Lelis, Felipe J. N. Travers, Meghan Habibi, Shaghayegh Pessaint, Laurent Van Ry, Alex Blade, Kelvin Brown, Renita Cook, Anthony Finneyfrock, Brad Dodson, Alan Teow, Elyse Velasco, Jason Zahn, Roland Wegmann, Frank Bondzie, Esther A. Dagotto, Gabriel Gebre, Makda S. He, Xuan Jacob-Dolan, Catherine Kirilova, Marinela Kordana, Nicole Lin, Zijin Maxfield, Lori F. Nampanya, Felix Nityanandam, Ramya Ventura, John D. Wan, Huahua Cai, Yongfei Chen, Bing Schmidt, Aaron G. Wesemann, Duane R. Baric, Ralph S. Alter, Galit Andersen, Hanne Lewis, Mark G. Barouch, Dan H. DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title | DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title_full | DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title_fullStr | DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title_full_unstemmed | DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title_short | DNA vaccine protection against SARS-CoV-2 in rhesus macaques |
| title_sort | dna vaccine protection against sars-cov-2 in rhesus macaques |
| topic | Research Articles |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7243363/ https://www.ncbi.nlm.nih.gov/pubmed/32434945 http://dx.doi.org/10.1126/science.abc6284 |
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