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Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression
The vital roles of long noncoding RNAs (lncRNAs) have been implicated in growing number of studies in tumor development. LncRNA CCAT1 has been recognized as associated with tumor development, yet its relation with colorectal cancer (CRC) remains elusive. Our study aimed at elucidating the function a...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244037/ https://www.ncbi.nlm.nih.gov/pubmed/32380476 http://dx.doi.org/10.18632/aging.103139 |
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author | Shang, Anquan Wang, Weiwei Gu, Chenzheng Chen, Wei Lu, Wenying Sun, Zujun Li, Dong |
author_facet | Shang, Anquan Wang, Weiwei Gu, Chenzheng Chen, Wei Lu, Wenying Sun, Zujun Li, Dong |
author_sort | Shang, Anquan |
collection | PubMed |
description | The vital roles of long noncoding RNAs (lncRNAs) have been implicated in growing number of studies in tumor development. LncRNA CCAT1 has been recognized as associated with tumor development, yet its relation with colorectal cancer (CRC) remains elusive. Our study aimed at elucidating the function and mechanisms of long non-coding RNA CCAT1 in CRC. From a lncRNA profile dataset of 38 pairs of matched tumor-control colon tissues from colorectal patients housed in The Cancer Genome Atlas (TCGA), we detected 10 upregulated and 10 down-regulated lncRNAs in CRC. Fifty cases of CRC patients were enrolled to analyze the correlation between the expression of CCAT1 and clinical pathology. The inverse correlation of expression and target relationship between CCAT1 and miR-181a-5p were verified using qRT-PCR and dual-luciferase reporter gene assay. Cell viability, colony formation ability, aggression and apoptosis were determined by MTT assay, colony formation assay, Transwell and wound healing assays and flow cytometry analysis. Furthermore, Xenograft model was used to show that knockdown of CCAT1 inhibits tumor growth in vivo. The expression of lncRNA CCAT1 was significantly upregulated in CRC tissues. The CCAT1 expression was positively associated with cancer stage (American Joint Committee on Cancer stage, P<0.05). CCAT1 promoted cell proliferation, growth and mobility by targeting miR-181a-5p and the silence of CCAT1 increased the cell apoptosis. Same effect was observed in an in vivo xenograft model, which the tumor size and pro-tumor proteins were significantly diminished by knocking down of CCAT1. |
format | Online Article Text |
id | pubmed-7244037 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-72440372020-06-03 Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression Shang, Anquan Wang, Weiwei Gu, Chenzheng Chen, Wei Lu, Wenying Sun, Zujun Li, Dong Aging (Albany NY) Research Paper The vital roles of long noncoding RNAs (lncRNAs) have been implicated in growing number of studies in tumor development. LncRNA CCAT1 has been recognized as associated with tumor development, yet its relation with colorectal cancer (CRC) remains elusive. Our study aimed at elucidating the function and mechanisms of long non-coding RNA CCAT1 in CRC. From a lncRNA profile dataset of 38 pairs of matched tumor-control colon tissues from colorectal patients housed in The Cancer Genome Atlas (TCGA), we detected 10 upregulated and 10 down-regulated lncRNAs in CRC. Fifty cases of CRC patients were enrolled to analyze the correlation between the expression of CCAT1 and clinical pathology. The inverse correlation of expression and target relationship between CCAT1 and miR-181a-5p were verified using qRT-PCR and dual-luciferase reporter gene assay. Cell viability, colony formation ability, aggression and apoptosis were determined by MTT assay, colony formation assay, Transwell and wound healing assays and flow cytometry analysis. Furthermore, Xenograft model was used to show that knockdown of CCAT1 inhibits tumor growth in vivo. The expression of lncRNA CCAT1 was significantly upregulated in CRC tissues. The CCAT1 expression was positively associated with cancer stage (American Joint Committee on Cancer stage, P<0.05). CCAT1 promoted cell proliferation, growth and mobility by targeting miR-181a-5p and the silence of CCAT1 increased the cell apoptosis. Same effect was observed in an in vivo xenograft model, which the tumor size and pro-tumor proteins were significantly diminished by knocking down of CCAT1. Impact Journals 2020-05-07 /pmc/articles/PMC7244037/ /pubmed/32380476 http://dx.doi.org/10.18632/aging.103139 Text en Copyright © 2020 Shang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Shang, Anquan Wang, Weiwei Gu, Chenzheng Chen, Wei Lu, Wenying Sun, Zujun Li, Dong Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title | Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title_full | Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title_fullStr | Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title_full_unstemmed | Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title_short | Long non-coding RNA CCAT1 promotes colorectal cancer progression by regulating miR-181a-5p expression |
title_sort | long non-coding rna ccat1 promotes colorectal cancer progression by regulating mir-181a-5p expression |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244037/ https://www.ncbi.nlm.nih.gov/pubmed/32380476 http://dx.doi.org/10.18632/aging.103139 |
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