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Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss

The blood exhibits a dynamic flux of proteins that are secreted by the tissues and cells of the body. To identify novel aging-related circulating proteins, we compared the plasma proteomic profiles of young and old mice using tandem mass spectrometry. The expression of 134 proteins differed between...

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Autores principales: Yang, Yong Ryoul, Kabir, Mohammad Humayun, Park, Jin Hee, Park, Jae-Il, Kang, Jae Sook, Ju, Shinyeong, Shin, Yeo Jin, Lee, Seung Min, Lee, Jaemin, Kim, Seokho, Lee, Kwang-Pyo, Lee, Soo Young, Lee, Cheolju, Kwon, Ki-Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244053/
https://www.ncbi.nlm.nih.gov/pubmed/32396872
http://dx.doi.org/10.18632/aging.103184
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author Yang, Yong Ryoul
Kabir, Mohammad Humayun
Park, Jin Hee
Park, Jae-Il
Kang, Jae Sook
Ju, Shinyeong
Shin, Yeo Jin
Lee, Seung Min
Lee, Jaemin
Kim, Seokho
Lee, Kwang-Pyo
Lee, Soo Young
Lee, Cheolju
Kwon, Ki-Sun
author_facet Yang, Yong Ryoul
Kabir, Mohammad Humayun
Park, Jin Hee
Park, Jae-Il
Kang, Jae Sook
Ju, Shinyeong
Shin, Yeo Jin
Lee, Seung Min
Lee, Jaemin
Kim, Seokho
Lee, Kwang-Pyo
Lee, Soo Young
Lee, Cheolju
Kwon, Ki-Sun
author_sort Yang, Yong Ryoul
collection PubMed
description The blood exhibits a dynamic flux of proteins that are secreted by the tissues and cells of the body. To identify novel aging-related circulating proteins, we compared the plasma proteomic profiles of young and old mice using tandem mass spectrometry. The expression of 134 proteins differed between young and old mice. We selected seven proteins that were expressed at higher levels in young mice, and confirmed their plasma expression in immunoassays. The plasma levels of anthrax toxin receptor 2 (ANTXR2), cadherin-13 (CDH-13), scavenger receptor cysteine-rich type 1 protein M130 (CD163), cartilage oligomeric matrix protein (COMP), Dickkopf-related protein 3 (DKK3), periostin, and secretogranin-1 were all confirmed to decrease with age. We then investigated whether any of the secreted proteins influenced bone metabolism and found that CDH-13 inhibited osteoclast differentiation. CDH 13 treatment suppressed the receptor activator of NF-κB ligand (RANKL) signaling pathway in bone marrow-derived macrophages, and intraperitoneal administration of CDH-13 delayed age-related bone loss in the femurs of aged mice. These findings suggest that low plasma CDH-13 expression in aged mice promotes aging-associated osteopenia by facilitating excessive osteoclast formation. Thus, CDH-13 could have therapeutic potential as a protein drug for the prevention of osteopenia.
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spelling pubmed-72440532020-06-03 Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss Yang, Yong Ryoul Kabir, Mohammad Humayun Park, Jin Hee Park, Jae-Il Kang, Jae Sook Ju, Shinyeong Shin, Yeo Jin Lee, Seung Min Lee, Jaemin Kim, Seokho Lee, Kwang-Pyo Lee, Soo Young Lee, Cheolju Kwon, Ki-Sun Aging (Albany NY) Research Paper The blood exhibits a dynamic flux of proteins that are secreted by the tissues and cells of the body. To identify novel aging-related circulating proteins, we compared the plasma proteomic profiles of young and old mice using tandem mass spectrometry. The expression of 134 proteins differed between young and old mice. We selected seven proteins that were expressed at higher levels in young mice, and confirmed their plasma expression in immunoassays. The plasma levels of anthrax toxin receptor 2 (ANTXR2), cadherin-13 (CDH-13), scavenger receptor cysteine-rich type 1 protein M130 (CD163), cartilage oligomeric matrix protein (COMP), Dickkopf-related protein 3 (DKK3), periostin, and secretogranin-1 were all confirmed to decrease with age. We then investigated whether any of the secreted proteins influenced bone metabolism and found that CDH-13 inhibited osteoclast differentiation. CDH 13 treatment suppressed the receptor activator of NF-κB ligand (RANKL) signaling pathway in bone marrow-derived macrophages, and intraperitoneal administration of CDH-13 delayed age-related bone loss in the femurs of aged mice. These findings suggest that low plasma CDH-13 expression in aged mice promotes aging-associated osteopenia by facilitating excessive osteoclast formation. Thus, CDH-13 could have therapeutic potential as a protein drug for the prevention of osteopenia. Impact Journals 2020-05-12 /pmc/articles/PMC7244053/ /pubmed/32396872 http://dx.doi.org/10.18632/aging.103184 Text en Copyright © 2020 Yang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Yang, Yong Ryoul
Kabir, Mohammad Humayun
Park, Jin Hee
Park, Jae-Il
Kang, Jae Sook
Ju, Shinyeong
Shin, Yeo Jin
Lee, Seung Min
Lee, Jaemin
Kim, Seokho
Lee, Kwang-Pyo
Lee, Soo Young
Lee, Cheolju
Kwon, Ki-Sun
Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title_full Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title_fullStr Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title_full_unstemmed Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title_short Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
title_sort plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244053/
https://www.ncbi.nlm.nih.gov/pubmed/32396872
http://dx.doi.org/10.18632/aging.103184
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