Cryo-EM structures from sub-nl volumes using pin-printing and jet vitrification

The increasing demand for cryo-electron microscopy (cryo-EM) reveals drawbacks in current sample preparation protocols, such as sample waste and lack of reproducibility. Here, we present several technical developments that provide efficient sample preparation for cryo-EM studies. Pin printing substa...

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Detalles Bibliográficos
Autores principales: Ravelli, Raimond B. G., Nijpels, Frank J. T., Henderikx, Rene J. M., Weissenberger, Giulia, Thewessem, Sanne, Gijsbers, Abril, Beulen, Bart W. A. M. M., López-Iglesias, Carmen, Peters, Peter J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244535/
https://www.ncbi.nlm.nih.gov/pubmed/32444637
http://dx.doi.org/10.1038/s41467-020-16392-5

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