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Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16

BACKGROUND: The purpose of this study was to determine the prevalence and genotype of Cryptosporidium spp. in different groups of immunocompromised patients admitted to the referral hospitals in center of Iran during 2015–2016. METHODS: This cross-sectional study was conducted on 346 immunocompromis...

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Autores principales: IZADI, Shahrokh, MOHAGHEGH, Mohammad Ali, GHAYOUR-NAJAFABADI, Zahra, AZAMI, Mehdi, MIRZAEI, Farzaneh, NAMDAR, Fatemeh, MOHEBALI, Mehdi, LESHAN WANNIGAMA, Dhammika, HEJAZI, Seyed-Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244833/
https://www.ncbi.nlm.nih.gov/pubmed/32489373
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author IZADI, Shahrokh
MOHAGHEGH, Mohammad Ali
GHAYOUR-NAJAFABADI, Zahra
AZAMI, Mehdi
MIRZAEI, Farzaneh
NAMDAR, Fatemeh
MOHEBALI, Mehdi
LESHAN WANNIGAMA, Dhammika
HEJAZI, Seyed-Hossein
author_facet IZADI, Shahrokh
MOHAGHEGH, Mohammad Ali
GHAYOUR-NAJAFABADI, Zahra
AZAMI, Mehdi
MIRZAEI, Farzaneh
NAMDAR, Fatemeh
MOHEBALI, Mehdi
LESHAN WANNIGAMA, Dhammika
HEJAZI, Seyed-Hossein
author_sort IZADI, Shahrokh
collection PubMed
description BACKGROUND: The purpose of this study was to determine the prevalence and genotype of Cryptosporidium spp. in different groups of immunocompromised patients admitted to the referral hospitals in center of Iran during 2015–2016. METHODS: This cross-sectional study was conducted on 346 immunocompromised patients (HIV(+)/AIDS, Lymphoma, Leukemia and organ transplants) in referred hospitals from central parts of Iran including Isfahan, Markazi, Yazd and Chaharmahale Bakhtiari provinces. Stool samples were analyzed for Cryptosporidium species, modified Ziehl–Neelsen staining techniques followed by the semi-nested PCR and DNA sequencing methods. RESULTS: The total rate of Cryptosporidium spp. was 3.46% (12/346) in the patients, however, the prevalence of the parasite, was 4.6% (4/87) in HIV(+)/AIDS patients, 3.6% (6/168) in patients with blood malignancy and 2.1% (2/91) in organ transplant recipients. The SSU rRNA gene of Cryptosporidium spp. in all microscopic-positive samples was effectively amplified by the semi-nested PCR and DNA sequences, exposed the existence of two Cryptosporidium species, including C. hominis 91.6% (11/12) and C. parvum 8.3% (1/12). CONCLUSION: The predominance of C. hominis in the present study may be certifies the importance of anthroponotic transmission of cryptosporidiosis in center of Iran.
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spelling pubmed-72448332020-06-01 Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16 IZADI, Shahrokh MOHAGHEGH, Mohammad Ali GHAYOUR-NAJAFABADI, Zahra AZAMI, Mehdi MIRZAEI, Farzaneh NAMDAR, Fatemeh MOHEBALI, Mehdi LESHAN WANNIGAMA, Dhammika HEJAZI, Seyed-Hossein Iran J Parasitol Original Article BACKGROUND: The purpose of this study was to determine the prevalence and genotype of Cryptosporidium spp. in different groups of immunocompromised patients admitted to the referral hospitals in center of Iran during 2015–2016. METHODS: This cross-sectional study was conducted on 346 immunocompromised patients (HIV(+)/AIDS, Lymphoma, Leukemia and organ transplants) in referred hospitals from central parts of Iran including Isfahan, Markazi, Yazd and Chaharmahale Bakhtiari provinces. Stool samples were analyzed for Cryptosporidium species, modified Ziehl–Neelsen staining techniques followed by the semi-nested PCR and DNA sequencing methods. RESULTS: The total rate of Cryptosporidium spp. was 3.46% (12/346) in the patients, however, the prevalence of the parasite, was 4.6% (4/87) in HIV(+)/AIDS patients, 3.6% (6/168) in patients with blood malignancy and 2.1% (2/91) in organ transplant recipients. The SSU rRNA gene of Cryptosporidium spp. in all microscopic-positive samples was effectively amplified by the semi-nested PCR and DNA sequences, exposed the existence of two Cryptosporidium species, including C. hominis 91.6% (11/12) and C. parvum 8.3% (1/12). CONCLUSION: The predominance of C. hominis in the present study may be certifies the importance of anthroponotic transmission of cryptosporidiosis in center of Iran. Tehran University of Medical Sciences 2020 /pmc/articles/PMC7244833/ /pubmed/32489373 Text en Copyright© Iranian Society of Parasitology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
IZADI, Shahrokh
MOHAGHEGH, Mohammad Ali
GHAYOUR-NAJAFABADI, Zahra
AZAMI, Mehdi
MIRZAEI, Farzaneh
NAMDAR, Fatemeh
MOHEBALI, Mehdi
LESHAN WANNIGAMA, Dhammika
HEJAZI, Seyed-Hossein
Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title_full Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title_fullStr Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title_full_unstemmed Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title_short Frequency and Molecular Identification of Cryptosporidium Species among Immunocompromised Patients Referred to Hospitals, Central Iran, 2015–16
title_sort frequency and molecular identification of cryptosporidium species among immunocompromised patients referred to hospitals, central iran, 2015–16
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7244833/
https://www.ncbi.nlm.nih.gov/pubmed/32489373
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