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Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis
The low cost lipase derived from Thermomyces lanugionous was chosen to conjugate with Fe(3)O(4) nanoparitcles as a magnetic responsive lipase (MRL) biocatalyst. The structure of MRL was observed by atomic force microscopy (AFM). The Fourier transform infrared (FTIR) spectroscopy analysis confirmed t...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Ltd.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245248/ https://www.ncbi.nlm.nih.gov/pubmed/32837987 http://dx.doi.org/10.1016/j.mtcomm.2020.101197 |
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author | Li, Jing Zhang, Jiandong Shen, Shuguang Zhang, Bing Yu, William W. |
author_facet | Li, Jing Zhang, Jiandong Shen, Shuguang Zhang, Bing Yu, William W. |
author_sort | Li, Jing |
collection | PubMed |
description | The low cost lipase derived from Thermomyces lanugionous was chosen to conjugate with Fe(3)O(4) nanoparitcles as a magnetic responsive lipase (MRL) biocatalyst. The structure of MRL was observed by atomic force microscopy (AFM). The Fourier transform infrared (FTIR) spectroscopy analysis confirmed the lipase conjugated to Fe(3)O(4) nanoparticles. Optimized conditions for the process of biodiesel production by MRL were investigated by the response surface methodology (RSM) and the Box-Behnken design (BBD). The optimized conditions for biodiesel production by MRL were as follows. The molar ratio of methanol to oil was 4.0, water content was 1.5 % as oil weight, the dosage of MRL to oil was 9.0 % (W/W) under 41 °C for 28 h. Under the optimized conditions, the yield of FAMEs by MRL reached 82.20 %. Further experiments showed that the MRL could be used 10 cycles and the yield of FAMEs decreased slightly by 10.97 %. These results indicated that Fe(3)O(4) nanoparticle carrier could efficiently improve the FAMEs synthesis and enhance the MRL stabilization and reusability in the biodiesel production. |
format | Online Article Text |
id | pubmed-7245248 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72452482020-05-26 Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis Li, Jing Zhang, Jiandong Shen, Shuguang Zhang, Bing Yu, William W. Mater Today Commun Article The low cost lipase derived from Thermomyces lanugionous was chosen to conjugate with Fe(3)O(4) nanoparitcles as a magnetic responsive lipase (MRL) biocatalyst. The structure of MRL was observed by atomic force microscopy (AFM). The Fourier transform infrared (FTIR) spectroscopy analysis confirmed the lipase conjugated to Fe(3)O(4) nanoparticles. Optimized conditions for the process of biodiesel production by MRL were investigated by the response surface methodology (RSM) and the Box-Behnken design (BBD). The optimized conditions for biodiesel production by MRL were as follows. The molar ratio of methanol to oil was 4.0, water content was 1.5 % as oil weight, the dosage of MRL to oil was 9.0 % (W/W) under 41 °C for 28 h. Under the optimized conditions, the yield of FAMEs by MRL reached 82.20 %. Further experiments showed that the MRL could be used 10 cycles and the yield of FAMEs decreased slightly by 10.97 %. These results indicated that Fe(3)O(4) nanoparticle carrier could efficiently improve the FAMEs synthesis and enhance the MRL stabilization and reusability in the biodiesel production. Elsevier Ltd. 2020-09 2020-05-23 /pmc/articles/PMC7245248/ /pubmed/32837987 http://dx.doi.org/10.1016/j.mtcomm.2020.101197 Text en © 2020 Elsevier Ltd. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Li, Jing Zhang, Jiandong Shen, Shuguang Zhang, Bing Yu, William W. Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title | Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title_full | Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title_fullStr | Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title_full_unstemmed | Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title_short | Magnetic responsive Thermomyces lanuginosus lipase for biodiesel synthesis |
title_sort | magnetic responsive thermomyces lanuginosus lipase for biodiesel synthesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245248/ https://www.ncbi.nlm.nih.gov/pubmed/32837987 http://dx.doi.org/10.1016/j.mtcomm.2020.101197 |
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