Cargando…

Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology

INTRODUCTION: Streptococcus pneumoniae is one of the frequently isolated organisms and an important aetiological agent of invasive bacterial diseases (IBD) like pneumonia, meningitis and sepsis. As a measure to control the burden of IBD, the Government of India introduced Pneumoccocal Conjugate Vacc...

Descripción completa

Detalles Bibliográficos
Autores principales: Rajkumar, Prabu, Bharathy, Sukumar, Girish Kumar, C P, Veeraraghavan, Balaji, Verghese, Valsan, Gupta, Nivedita, Kangusamy, Boopathi, Ravi, Muthusamy, Jayaraman, Yuvaraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMJ Publishing Group 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245370/
https://www.ncbi.nlm.nih.gov/pubmed/32273315
http://dx.doi.org/10.1136/bmjopen-2019-034663
_version_ 1783537743442214912
author Rajkumar, Prabu
Bharathy, Sukumar
Girish Kumar, C P
Veeraraghavan, Balaji
Verghese, Valsan
Gupta, Nivedita
Kangusamy, Boopathi
Ravi, Muthusamy
Jayaraman, Yuvaraj
author_facet Rajkumar, Prabu
Bharathy, Sukumar
Girish Kumar, C P
Veeraraghavan, Balaji
Verghese, Valsan
Gupta, Nivedita
Kangusamy, Boopathi
Ravi, Muthusamy
Jayaraman, Yuvaraj
author_sort Rajkumar, Prabu
collection PubMed
description INTRODUCTION: Streptococcus pneumoniae is one of the frequently isolated organisms and an important aetiological agent of invasive bacterial diseases (IBD) like pneumonia, meningitis and sepsis. As a measure to control the burden of IBD, the Government of India introduced Pneumoccocal Conjugate Vaccine-13 (PCV-13) in the Universal Immunization Program in high burden districts of five states in a phased manner from 2017 onwards. It is essential to understand the trend of circulating pneumococcal serotypes associated with IBD in the prevaccination and postvaccination scenarios to decide on the expansion of vaccination programmes and PCV reformulation. This manuscript describes the protocol for hospital-based sentinel surveillance for S. pneumoniae and other organisms causing IBD across various geographical regions in India. METHODS AND ANALYSIS: Hospital-based surveillance is established in selected hospitals to recruit children aged 1–59 months with symptoms of pneumonia and other IBD. Diagnostic criteria were adapted from standard WHO case definitions. Case Report Forms (CRFs) are used to collect data from the enrolled children. Blood, cerebrospinal fluid (CSF) and other normally sterile body fluids are collected and subjected to microscopy, cytology, latex agglutination, biochemistry, bacteriological culture and real-time PCR as applicable. Pneumococcal isolates are serotyped and tested for assessing antimicrobial resistance patterns. Data will be analysed by simple descriptive statistics to estimate the proportion of pneumonia and other IBD due to S. pneumoniae, Hemophilus influenzae type b and Neisseria meningitidis. Prevalence of bacterial infection, circulating pneumococcal serotypes, antibiotic resistance patterns, serotype variability across seasons and regions will be described in terms of percentage with 95% confidence interval. ETHICS AND DISSEMINATION: The institutional review boards of the coordinating centre, all sentinel sites, regional and national reference laboratories approved the project. The results will be published in peer-reviewed journals and shared with stakeholders for deciding on revising vaccination strategy appropriately.
format Online
Article
Text
id pubmed-7245370
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher BMJ Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-72453702020-06-03 Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology Rajkumar, Prabu Bharathy, Sukumar Girish Kumar, C P Veeraraghavan, Balaji Verghese, Valsan Gupta, Nivedita Kangusamy, Boopathi Ravi, Muthusamy Jayaraman, Yuvaraj BMJ Open Public Health INTRODUCTION: Streptococcus pneumoniae is one of the frequently isolated organisms and an important aetiological agent of invasive bacterial diseases (IBD) like pneumonia, meningitis and sepsis. As a measure to control the burden of IBD, the Government of India introduced Pneumoccocal Conjugate Vaccine-13 (PCV-13) in the Universal Immunization Program in high burden districts of five states in a phased manner from 2017 onwards. It is essential to understand the trend of circulating pneumococcal serotypes associated with IBD in the prevaccination and postvaccination scenarios to decide on the expansion of vaccination programmes and PCV reformulation. This manuscript describes the protocol for hospital-based sentinel surveillance for S. pneumoniae and other organisms causing IBD across various geographical regions in India. METHODS AND ANALYSIS: Hospital-based surveillance is established in selected hospitals to recruit children aged 1–59 months with symptoms of pneumonia and other IBD. Diagnostic criteria were adapted from standard WHO case definitions. Case Report Forms (CRFs) are used to collect data from the enrolled children. Blood, cerebrospinal fluid (CSF) and other normally sterile body fluids are collected and subjected to microscopy, cytology, latex agglutination, biochemistry, bacteriological culture and real-time PCR as applicable. Pneumococcal isolates are serotyped and tested for assessing antimicrobial resistance patterns. Data will be analysed by simple descriptive statistics to estimate the proportion of pneumonia and other IBD due to S. pneumoniae, Hemophilus influenzae type b and Neisseria meningitidis. Prevalence of bacterial infection, circulating pneumococcal serotypes, antibiotic resistance patterns, serotype variability across seasons and regions will be described in terms of percentage with 95% confidence interval. ETHICS AND DISSEMINATION: The institutional review boards of the coordinating centre, all sentinel sites, regional and national reference laboratories approved the project. The results will be published in peer-reviewed journals and shared with stakeholders for deciding on revising vaccination strategy appropriately. BMJ Publishing Group 2020-04-08 /pmc/articles/PMC7245370/ /pubmed/32273315 http://dx.doi.org/10.1136/bmjopen-2019-034663 Text en © Author(s) (or their employer(s)) 2020. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ. http://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.
spellingShingle Public Health
Rajkumar, Prabu
Bharathy, Sukumar
Girish Kumar, C P
Veeraraghavan, Balaji
Verghese, Valsan
Gupta, Nivedita
Kangusamy, Boopathi
Ravi, Muthusamy
Jayaraman, Yuvaraj
Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title_full Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title_fullStr Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title_full_unstemmed Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title_short Hospital-based sentinel surveillance for Streptococcus pneumoniae and other invasive bacterial diseases in India (HBSSPIBD): design and methodology
title_sort hospital-based sentinel surveillance for streptococcus pneumoniae and other invasive bacterial diseases in india (hbsspibd): design and methodology
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7245370/
https://www.ncbi.nlm.nih.gov/pubmed/32273315
http://dx.doi.org/10.1136/bmjopen-2019-034663
work_keys_str_mv AT rajkumarprabu hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT bharathysukumar hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT girishkumarcp hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT veeraraghavanbalaji hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT verghesevalsan hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT guptanivedita hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT kangusamyboopathi hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT ravimuthusamy hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT jayaramanyuvaraj hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology
AT hospitalbasedsentinelsurveillanceforstreptococcuspneumoniaeandotherinvasivebacterialdiseasesinindiahbsspibddesignandmethodology