Cargando…
Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer
BACKGROUND: GLOBOCAN 2018 latest data show cervical cancer ranks fourth in morbidity and mortality among women. Many genes in cervical lesions differ in sensitivity and specificity. However, the diagnostic molecules for early cervical cancer are not very clear. This paper screens biomarkers for earl...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246350/ https://www.ncbi.nlm.nih.gov/pubmed/31976596 http://dx.doi.org/10.1002/jcla.23207 |
_version_ | 1783537925637537792 |
---|---|
author | Huo, Wenyan Zhai, Shuaiyu Wang, Yanbo Qiang, Xin Na, Risu Gui, Hua Wu, Ningjin Cao, Yaning Bai, Haihua |
author_facet | Huo, Wenyan Zhai, Shuaiyu Wang, Yanbo Qiang, Xin Na, Risu Gui, Hua Wu, Ningjin Cao, Yaning Bai, Haihua |
author_sort | Huo, Wenyan |
collection | PubMed |
description | BACKGROUND: GLOBOCAN 2018 latest data show cervical cancer ranks fourth in morbidity and mortality among women. Many genes in cervical lesions differ in sensitivity and specificity. However, the diagnostic molecules for early cervical cancer are not very clear. This paper screens biomarkers for early molecular diagnosis of Mongolian patients with cervical cancer. METHODS: Immunohistochemical SP method was used to detect the expression of p16(INK4a) and Notch1 protein in paraffin sections of 226 Mongolian patients with HPV16‐positive cervical lesions after pathological examination, and 100 of them were randomly selected by fluorescence in situ hybridization to detect hTERC gene. The HPV16‐binding human cervical cancer SiHa cell line was used to silence the expression of HPV16 E6/E7 gene by RNA interference, and the expression of p16(INK4a), Notch1, and hTERC genes and protein expression levels were detected by RT‐PCR and Western blot. RESULTS: The positive expression rates of p16(INK4a), Notch1, and hTERC genes in HPV16‐positive cervical cancer, CIN‐III, CIN‐II, CIN‐I, uterine leiomyoma, and chronic cervicitis were significantly different (P < .05); the positive expression rates of the three genes were also significantly different in the same type of cervical lesions (P < .05); RNA interference can effectively inhibit HPV16 E6/E7, p16(INK4a) and Notch1 gene expression, but has no effect on hTERC gene expression. CONCLUSION: The p16(INK4a) gene can be used as a biomarker for early screening of cervical cancer, and the hTERC gene can be used to confirm the clinical diagnosis of cervical cancer. |
format | Online Article Text |
id | pubmed-7246350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72463502020-06-01 Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer Huo, Wenyan Zhai, Shuaiyu Wang, Yanbo Qiang, Xin Na, Risu Gui, Hua Wu, Ningjin Cao, Yaning Bai, Haihua J Clin Lab Anal Research Articles BACKGROUND: GLOBOCAN 2018 latest data show cervical cancer ranks fourth in morbidity and mortality among women. Many genes in cervical lesions differ in sensitivity and specificity. However, the diagnostic molecules for early cervical cancer are not very clear. This paper screens biomarkers for early molecular diagnosis of Mongolian patients with cervical cancer. METHODS: Immunohistochemical SP method was used to detect the expression of p16(INK4a) and Notch1 protein in paraffin sections of 226 Mongolian patients with HPV16‐positive cervical lesions after pathological examination, and 100 of them were randomly selected by fluorescence in situ hybridization to detect hTERC gene. The HPV16‐binding human cervical cancer SiHa cell line was used to silence the expression of HPV16 E6/E7 gene by RNA interference, and the expression of p16(INK4a), Notch1, and hTERC genes and protein expression levels were detected by RT‐PCR and Western blot. RESULTS: The positive expression rates of p16(INK4a), Notch1, and hTERC genes in HPV16‐positive cervical cancer, CIN‐III, CIN‐II, CIN‐I, uterine leiomyoma, and chronic cervicitis were significantly different (P < .05); the positive expression rates of the three genes were also significantly different in the same type of cervical lesions (P < .05); RNA interference can effectively inhibit HPV16 E6/E7, p16(INK4a) and Notch1 gene expression, but has no effect on hTERC gene expression. CONCLUSION: The p16(INK4a) gene can be used as a biomarker for early screening of cervical cancer, and the hTERC gene can be used to confirm the clinical diagnosis of cervical cancer. John Wiley and Sons Inc. 2020-01-24 /pmc/articles/PMC7246350/ /pubmed/31976596 http://dx.doi.org/10.1002/jcla.23207 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Huo, Wenyan Zhai, Shuaiyu Wang, Yanbo Qiang, Xin Na, Risu Gui, Hua Wu, Ningjin Cao, Yaning Bai, Haihua Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title | Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title_full | Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title_fullStr | Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title_full_unstemmed | Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title_short | Relevance research between the expression of p16(INK4a), Notch1, and hTERC genes: The development of HPV16‐positive cervical cancer |
title_sort | relevance research between the expression of p16(ink4a), notch1, and hterc genes: the development of hpv16‐positive cervical cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246350/ https://www.ncbi.nlm.nih.gov/pubmed/31976596 http://dx.doi.org/10.1002/jcla.23207 |
work_keys_str_mv | AT huowenyan relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT zhaishuaiyu relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT wangyanbo relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT qiangxin relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT narisu relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT guihua relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT wuningjin relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT caoyaning relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer AT baihaihua relevanceresearchbetweentheexpressionofp16ink4anotch1andhtercgenesthedevelopmentofhpv16positivecervicalcancer |