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MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer
BACKGROUND: Nucleolar and spindle‐associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246360/ https://www.ncbi.nlm.nih.gov/pubmed/31901150 http://dx.doi.org/10.1002/jcla.23193 |
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author | Chen, Yifan Zhang, Wentao Kadier, Aimaitiaji Zhang, Haimin Yao, Xudong |
author_facet | Chen, Yifan Zhang, Wentao Kadier, Aimaitiaji Zhang, Haimin Yao, Xudong |
author_sort | Chen, Yifan |
collection | PubMed |
description | BACKGROUND: Nucleolar and spindle‐associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioinformatics methods, we found that NUSAP1 was a putative target of miR‐769‐5p. Here, we aimed to explore whether miR‐769‐5p is involved in bladder cancer progression via targeting NUSAP1. METHODS: MiR‐769‐5p expression patterns in bladder cancer tissues and cells were detected by RT‐PCR. Kaplan‐Meier was used to determine the clinical effects of miR‐769‐5p expression levels on the overall survival of bladder cancer patients. Bioinformatics methods were used to predict the binding sites between miR‐769‐5p and NUSAP1, which was verified by the luciferase gene reporter assay. CCK‐8, flow cytometry, wound healing and transwell chamber experiments were performed to test cell growth, apoptosis, migration and invasion capacities. RESULTS: miR‐769‐5p was lowly expressed in bladder cancer tissues and cells, which was closely associated with poor prognosis. Overexpression of miR‐769‐5p induced significant repressions in cell growth, migration, and invasion and caused an obvious increase in cell apoptosis, whereas these tendencies were reversed when NUSAP1 was upregulated. CONCLUSION: This study demonstrates that miR‐769‐5p functions as a tumor suppressor in bladder cancer via targeting NUSAP1. |
format | Online Article Text |
id | pubmed-7246360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-72463602020-06-01 MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer Chen, Yifan Zhang, Wentao Kadier, Aimaitiaji Zhang, Haimin Yao, Xudong J Clin Lab Anal Research Articles BACKGROUND: Nucleolar and spindle‐associated protein 1 (NUSAP1) has been identified to be strongly implicated in the carcinogenesis of cervical carcinoma, breast cancer, and liver cancer, and shows a high expression level in bladder cancer, indicating that NUSAP1 might be a potent target for cancer treatment. Using bioinformatics methods, we found that NUSAP1 was a putative target of miR‐769‐5p. Here, we aimed to explore whether miR‐769‐5p is involved in bladder cancer progression via targeting NUSAP1. METHODS: MiR‐769‐5p expression patterns in bladder cancer tissues and cells were detected by RT‐PCR. Kaplan‐Meier was used to determine the clinical effects of miR‐769‐5p expression levels on the overall survival of bladder cancer patients. Bioinformatics methods were used to predict the binding sites between miR‐769‐5p and NUSAP1, which was verified by the luciferase gene reporter assay. CCK‐8, flow cytometry, wound healing and transwell chamber experiments were performed to test cell growth, apoptosis, migration and invasion capacities. RESULTS: miR‐769‐5p was lowly expressed in bladder cancer tissues and cells, which was closely associated with poor prognosis. Overexpression of miR‐769‐5p induced significant repressions in cell growth, migration, and invasion and caused an obvious increase in cell apoptosis, whereas these tendencies were reversed when NUSAP1 was upregulated. CONCLUSION: This study demonstrates that miR‐769‐5p functions as a tumor suppressor in bladder cancer via targeting NUSAP1. John Wiley and Sons Inc. 2020-01-03 /pmc/articles/PMC7246360/ /pubmed/31901150 http://dx.doi.org/10.1002/jcla.23193 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Chen, Yifan Zhang, Wentao Kadier, Aimaitiaji Zhang, Haimin Yao, Xudong MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title | MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title_full | MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title_fullStr | MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title_full_unstemmed | MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title_short | MicroRNA‐769‐5p suppresses cell growth and migration via targeting NUSAP1 in bladder cancer |
title_sort | microrna‐769‐5p suppresses cell growth and migration via targeting nusap1 in bladder cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246360/ https://www.ncbi.nlm.nih.gov/pubmed/31901150 http://dx.doi.org/10.1002/jcla.23193 |
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