Cargando…

Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes

BACKGROUND: In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non‐coding RNAE330013P06 has been shown to be upregulated in diabetes, and long...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Runqi, Shi, Pengcheng, Zhang, Yan, Wu, Haiming, Li, Xiaoping, Yang, Wengfu, Luo, Fei, JinmingXue, Yao, Liang, Yang, Jun, Wang, Wangfu, Zhang, Bo, Li, Peng, Miao, Yongmin, Wang, Qianjun, Tian, Fuguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246379/
https://www.ncbi.nlm.nih.gov/pubmed/31907990
http://dx.doi.org/10.1002/jcla.23172
_version_ 1783537932205817856
author Chen, Runqi
Shi, Pengcheng
Zhang, Yan
Wu, Haiming
Li, Xiaoping
Yang, Wengfu
Luo, Fei
JinmingXue,
Yao, Liang
Yang, Jun
Wang, Wangfu
Zhang, Bo
Li, Peng
Miao, Yongmin
Wang, Qianjun
Tian, Fuguo
author_facet Chen, Runqi
Shi, Pengcheng
Zhang, Yan
Wu, Haiming
Li, Xiaoping
Yang, Wengfu
Luo, Fei
JinmingXue,
Yao, Liang
Yang, Jun
Wang, Wangfu
Zhang, Bo
Li, Peng
Miao, Yongmin
Wang, Qianjun
Tian, Fuguo
author_sort Chen, Runqi
collection PubMed
description BACKGROUND: In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non‐coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non‐coding RNAs generally promote progression of cancer. METHODS: About 200 specimens of breast patients were obtained in previous clinical trial; 34 samples diagnosed as type 2 diabetes in breast cancer patient were enrolled in this research. Blood samples from 36 patients diagnosed as breast cancer without diabetes; 35 diabetic patients and 35 healthy peoples were obtained as control. All blood samples were measured by quantitative real‐time PCR (qRT‐PCR). Invasion and migration were tested by Transwell assay. Cell proliferation assay was tested by CCK‐8. Protein analysis was determined by Western blot. RESULTS: Compared with breast cancer patients without diabetes, diabetic patients without breast cancer and healthy peoples, LncRNAE330013P06 was upregulated in breast cancer patient with diabetes. Furthermore, of 34 breast patients, high LncRNAE330013P06 expression was significantly associated with family history, tumor‐node‐metastasis stage and lymph node metastasis. E33 promoted cancer cell growth in vitro via downregulation of P53. CONCLUSION: Upregulation of LncRNAE330013P06 driven by type 2 diabetes is one of the factors which promoted progression of breast cancer.
format Online
Article
Text
id pubmed-7246379
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-72463792020-06-01 Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes Chen, Runqi Shi, Pengcheng Zhang, Yan Wu, Haiming Li, Xiaoping Yang, Wengfu Luo, Fei JinmingXue, Yao, Liang Yang, Jun Wang, Wangfu Zhang, Bo Li, Peng Miao, Yongmin Wang, Qianjun Tian, Fuguo J Clin Lab Anal Research Articles BACKGROUND: In previous research, we found diabetes rather than obesity was an independent risk factor of breast cancer. However, why diabetes could lead to increased risk of breast cancer patients remains elusive. Long non‐coding RNAE330013P06 has been shown to be upregulated in diabetes, and long non‐coding RNAs generally promote progression of cancer. METHODS: About 200 specimens of breast patients were obtained in previous clinical trial; 34 samples diagnosed as type 2 diabetes in breast cancer patient were enrolled in this research. Blood samples from 36 patients diagnosed as breast cancer without diabetes; 35 diabetic patients and 35 healthy peoples were obtained as control. All blood samples were measured by quantitative real‐time PCR (qRT‐PCR). Invasion and migration were tested by Transwell assay. Cell proliferation assay was tested by CCK‐8. Protein analysis was determined by Western blot. RESULTS: Compared with breast cancer patients without diabetes, diabetic patients without breast cancer and healthy peoples, LncRNAE330013P06 was upregulated in breast cancer patient with diabetes. Furthermore, of 34 breast patients, high LncRNAE330013P06 expression was significantly associated with family history, tumor‐node‐metastasis stage and lymph node metastasis. E33 promoted cancer cell growth in vitro via downregulation of P53. CONCLUSION: Upregulation of LncRNAE330013P06 driven by type 2 diabetes is one of the factors which promoted progression of breast cancer. John Wiley and Sons Inc. 2020-01-06 /pmc/articles/PMC7246379/ /pubmed/31907990 http://dx.doi.org/10.1002/jcla.23172 Text en © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals, Inc. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Chen, Runqi
Shi, Pengcheng
Zhang, Yan
Wu, Haiming
Li, Xiaoping
Yang, Wengfu
Luo, Fei
JinmingXue,
Yao, Liang
Yang, Jun
Wang, Wangfu
Zhang, Bo
Li, Peng
Miao, Yongmin
Wang, Qianjun
Tian, Fuguo
Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title_full Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title_fullStr Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title_full_unstemmed Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title_short Long non‐coding RNAE330013P06 promotes progression of breast cancer with type 2 diabetes
title_sort long non‐coding rnae330013p06 promotes progression of breast cancer with type 2 diabetes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246379/
https://www.ncbi.nlm.nih.gov/pubmed/31907990
http://dx.doi.org/10.1002/jcla.23172
work_keys_str_mv AT chenrunqi longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT shipengcheng longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT zhangyan longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT wuhaiming longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT lixiaoping longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT yangwengfu longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT luofei longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT jinmingxue longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT yaoliang longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT yangjun longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT wangwangfu longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT zhangbo longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT lipeng longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT miaoyongmin longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT wangqianjun longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes
AT tianfuguo longnoncodingrnae330013p06promotesprogressionofbreastcancerwithtype2diabetes