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The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli

Bacterial cell division is guided by filamenting temperature-sensitive Z (FtsZ) treadmilling at midcell. FtsZ itself is regulated by FtsZ-associated proteins (Zaps) that couple it to different cellular processes. Z-associated protein A (ZapA) is known to enhance FtsZ bundling but also forms a synchr...

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Autores principales: Meiresonne, Nils Y., den Blaauwen, Tanneke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246612/
https://www.ncbi.nlm.nih.gov/pubmed/32365468
http://dx.doi.org/10.3390/ijms21093130
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author Meiresonne, Nils Y.
den Blaauwen, Tanneke
author_facet Meiresonne, Nils Y.
den Blaauwen, Tanneke
author_sort Meiresonne, Nils Y.
collection PubMed
description Bacterial cell division is guided by filamenting temperature-sensitive Z (FtsZ) treadmilling at midcell. FtsZ itself is regulated by FtsZ-associated proteins (Zaps) that couple it to different cellular processes. Z-associated protein A (ZapA) is known to enhance FtsZ bundling but also forms a synchronizing link with chromosome segregation through Z-associated protein B (ZapB) and matS-bound MatP. ZapA likely exists as dimers and tetramers in the cell. Using a ZapA mutant that is only able to form dimers in vitro (ZapA(I83E)), this paper investigates the effects of ZapA multimerization state on its interaction partners and cell division. By employing fluorescence microscopy and Förster resonance energy transfer in vivo it was shown that ZapA(I83E) is unable to complement a zapA deletion strain and localizes diffusely through the cell but still interacts with FtsZ that is not part of the cell division machinery. The diffusely-localized ZapA(I83E) is unable to recruit ZapB, which in its presence localizes unipolarly. Interestingly, the localization profiles of the chromosome and unipolar ZapB anticorrelate. The work presented here confirms previously reported in vitro effects of ZapA multimerization in vivo and places it in a broader context by revealing the strong implications for ZapB and chromosome localization and ter linkage.
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spelling pubmed-72466122020-06-10 The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli Meiresonne, Nils Y. den Blaauwen, Tanneke Int J Mol Sci Article Bacterial cell division is guided by filamenting temperature-sensitive Z (FtsZ) treadmilling at midcell. FtsZ itself is regulated by FtsZ-associated proteins (Zaps) that couple it to different cellular processes. Z-associated protein A (ZapA) is known to enhance FtsZ bundling but also forms a synchronizing link with chromosome segregation through Z-associated protein B (ZapB) and matS-bound MatP. ZapA likely exists as dimers and tetramers in the cell. Using a ZapA mutant that is only able to form dimers in vitro (ZapA(I83E)), this paper investigates the effects of ZapA multimerization state on its interaction partners and cell division. By employing fluorescence microscopy and Förster resonance energy transfer in vivo it was shown that ZapA(I83E) is unable to complement a zapA deletion strain and localizes diffusely through the cell but still interacts with FtsZ that is not part of the cell division machinery. The diffusely-localized ZapA(I83E) is unable to recruit ZapB, which in its presence localizes unipolarly. Interestingly, the localization profiles of the chromosome and unipolar ZapB anticorrelate. The work presented here confirms previously reported in vitro effects of ZapA multimerization in vivo and places it in a broader context by revealing the strong implications for ZapB and chromosome localization and ter linkage. MDPI 2020-04-29 /pmc/articles/PMC7246612/ /pubmed/32365468 http://dx.doi.org/10.3390/ijms21093130 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Meiresonne, Nils Y.
den Blaauwen, Tanneke
The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title_full The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title_fullStr The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title_full_unstemmed The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title_short The In Vitro Non-Tetramerizing ZapA(I83E) Mutant Is Unable to Recruit ZapB to the Division Plane In Vivo in Escherichia coli
title_sort in vitro non-tetramerizing zapa(i83e) mutant is unable to recruit zapb to the division plane in vivo in escherichia coli
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246612/
https://www.ncbi.nlm.nih.gov/pubmed/32365468
http://dx.doi.org/10.3390/ijms21093130
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