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Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles

Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms. Even though the rapidly growing EVs research field has sparked great interest in the last 20 years,...

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Detalles Bibliográficos
Autores principales: Simon, Thomas, Kumaran, Anish, Veselu, Diana-Florentina, Giamas, Georgios
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246849/
https://www.ncbi.nlm.nih.gov/pubmed/32349218
http://dx.doi.org/10.3390/ijms21093071
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author Simon, Thomas
Kumaran, Anish
Veselu, Diana-Florentina
Giamas, Georgios
author_facet Simon, Thomas
Kumaran, Anish
Veselu, Diana-Florentina
Giamas, Georgios
author_sort Simon, Thomas
collection PubMed
description Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms. Even though the rapidly growing EVs research field has sparked great interest in the last 20 years, many biological and technical aspects still remain challenging. One of the main issues that the field is facing is the absence of consensus regarding methods for EVs concentration from biofluids and tissue culture medium. Yet, not only can classic methods be time consuming, commercialized kits are also often quite expensive, especially when research requires analyzing numerous samples or concentrating EVs from large sample volumes. In addition, EV concentration often results in either low final yield or significant contamination of the vesicle sample with proteins and protein complexes of similar densities and sizes. Eventually, low vesicle yields highly limit any further application and data reproducibility while contamination greatly impacts extensive functional studies. Hence, there is a need for accessible and sustainable methods for improved vesicle concentration as this is a critical step in any EVs-related research study. In this brief report, we describe a novel combination of three well-known methods in order to obtain moderate-to-high yields of EVs with reduced protein contamination. We believe that such methods could be of high benefits for in vitro and in vivo functional studies.
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spelling pubmed-72468492020-06-02 Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles Simon, Thomas Kumaran, Anish Veselu, Diana-Florentina Giamas, Georgios Int J Mol Sci Communication Extracellular vesicles (EVs) are nanosized structures able to carry proteins, lipids and genetic material from one cell to another with critical implications in intercellular communication mechanisms. Even though the rapidly growing EVs research field has sparked great interest in the last 20 years, many biological and technical aspects still remain challenging. One of the main issues that the field is facing is the absence of consensus regarding methods for EVs concentration from biofluids and tissue culture medium. Yet, not only can classic methods be time consuming, commercialized kits are also often quite expensive, especially when research requires analyzing numerous samples or concentrating EVs from large sample volumes. In addition, EV concentration often results in either low final yield or significant contamination of the vesicle sample with proteins and protein complexes of similar densities and sizes. Eventually, low vesicle yields highly limit any further application and data reproducibility while contamination greatly impacts extensive functional studies. Hence, there is a need for accessible and sustainable methods for improved vesicle concentration as this is a critical step in any EVs-related research study. In this brief report, we describe a novel combination of three well-known methods in order to obtain moderate-to-high yields of EVs with reduced protein contamination. We believe that such methods could be of high benefits for in vitro and in vivo functional studies. MDPI 2020-04-27 /pmc/articles/PMC7246849/ /pubmed/32349218 http://dx.doi.org/10.3390/ijms21093071 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Simon, Thomas
Kumaran, Anish
Veselu, Diana-Florentina
Giamas, Georgios
Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title_full Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title_fullStr Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title_full_unstemmed Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title_short Three Method-Combination Protocol for Improving Purity of Extracellular Vesicles
title_sort three method-combination protocol for improving purity of extracellular vesicles
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246849/
https://www.ncbi.nlm.nih.gov/pubmed/32349218
http://dx.doi.org/10.3390/ijms21093071
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