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The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway

Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host...

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Autores principales: Wei, Songhong, Wang, Yingling, Zhou, Jianming, Xiang, Shibo, Sun, Wenxian, Peng, Xunwen, Li, Jing, Hai, Yingfan, Wang, Yan, Li, Shuai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246986/
https://www.ncbi.nlm.nih.gov/pubmed/32397668
http://dx.doi.org/10.3390/ijms21093376
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author Wei, Songhong
Wang, Yingling
Zhou, Jianming
Xiang, Shibo
Sun, Wenxian
Peng, Xunwen
Li, Jing
Hai, Yingfan
Wang, Yan
Li, Shuai
author_facet Wei, Songhong
Wang, Yingling
Zhou, Jianming
Xiang, Shibo
Sun, Wenxian
Peng, Xunwen
Li, Jing
Hai, Yingfan
Wang, Yan
Li, Shuai
author_sort Wei, Songhong
collection PubMed
description Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we demonstrated a small secreted hypersensitive response-inducing protein (hrip), named UvHrip1, which was highly conserved in U. virens isolates. UvHrip1 was also proven to suppress necrosis-like defense symptoms in N. benthamiana induced by the oomycete elicitor INF1. The localization of UvHrip1 was mainly in the nuclei and cytoplasm via monitoring the UvHrip1-GFP fusion protein in rice cells. Furthermore, Y2H and BiFC assay demonstrated that UvHrip1 interacted with OsHGW, which is a critical regulator in heading date and grain weight signaling pathways in rice. Expression patterns of defense- and heading date-related genes, OsPR1#051 and OsMYB21, were down-regulated over U. virens infection in rice. Collectively, our data provide a theory for gaining an insight into the molecular mechanisms underlying the UvHrip1 virulence function.
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spelling pubmed-72469862020-06-02 The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway Wei, Songhong Wang, Yingling Zhou, Jianming Xiang, Shibo Sun, Wenxian Peng, Xunwen Li, Jing Hai, Yingfan Wang, Yan Li, Shuai Int J Mol Sci Article Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we demonstrated a small secreted hypersensitive response-inducing protein (hrip), named UvHrip1, which was highly conserved in U. virens isolates. UvHrip1 was also proven to suppress necrosis-like defense symptoms in N. benthamiana induced by the oomycete elicitor INF1. The localization of UvHrip1 was mainly in the nuclei and cytoplasm via monitoring the UvHrip1-GFP fusion protein in rice cells. Furthermore, Y2H and BiFC assay demonstrated that UvHrip1 interacted with OsHGW, which is a critical regulator in heading date and grain weight signaling pathways in rice. Expression patterns of defense- and heading date-related genes, OsPR1#051 and OsMYB21, were down-regulated over U. virens infection in rice. Collectively, our data provide a theory for gaining an insight into the molecular mechanisms underlying the UvHrip1 virulence function. MDPI 2020-05-10 /pmc/articles/PMC7246986/ /pubmed/32397668 http://dx.doi.org/10.3390/ijms21093376 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Songhong
Wang, Yingling
Zhou, Jianming
Xiang, Shibo
Sun, Wenxian
Peng, Xunwen
Li, Jing
Hai, Yingfan
Wang, Yan
Li, Shuai
The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title_full The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title_fullStr The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title_full_unstemmed The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title_short The Conserved Effector UvHrip1 Interacts with OsHGW and Infection of Ustilaginoidea virens Regulates Defense- and Heading Date-Related Signaling Pathway
title_sort conserved effector uvhrip1 interacts with oshgw and infection of ustilaginoidea virens regulates defense- and heading date-related signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246986/
https://www.ncbi.nlm.nih.gov/pubmed/32397668
http://dx.doi.org/10.3390/ijms21093376
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