Cargando…

Stability of Glutaraldehyde in Biocide Compositions

Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inhere...

Descripción completa

Detalles Bibliográficos
Autores principales: Matei, Alina, Puscas, Cristina, Patrascu, Iulia, Lehene, Maria, Ziebro, Julia, Scurtu, Florina, Baia, Monica, Porumb, Dan, Totos, Robert, Silaghi-Dumitrescu, Radu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246990/
https://www.ncbi.nlm.nih.gov/pubmed/32397667
http://dx.doi.org/10.3390/ijms21093372
_version_ 1783538075543011328
author Matei, Alina
Puscas, Cristina
Patrascu, Iulia
Lehene, Maria
Ziebro, Julia
Scurtu, Florina
Baia, Monica
Porumb, Dan
Totos, Robert
Silaghi-Dumitrescu, Radu
author_facet Matei, Alina
Puscas, Cristina
Patrascu, Iulia
Lehene, Maria
Ziebro, Julia
Scurtu, Florina
Baia, Monica
Porumb, Dan
Totos, Robert
Silaghi-Dumitrescu, Radu
author_sort Matei, Alina
collection PubMed
description Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inherent to GA. An investigation of experimental and theoretical data is reported here, assessing the stability of GA solutions relevant for biocide compositions. GA solutions of various chemical composition and under varying circumstances were analyzed using spectroscopy (UV-VIS, Raman, NMR) coupled with density functional theory (DFT) calculations, as well as chemically, such as via the formation of imines in reaction/titration with glycine monitored at 270 nm; using LC-MS; or using SDS-PAGE analysis with GA as reagent in the polymerization of two test proteins- hemoglobin and myoglobin. The spectral properties of GA changed significantly over time, in a temperature-dependent manner; titration with glycine confirmed the spectral data. SDS-PAGE experiments demonstrated a non-linear and apparently unpredictable change in the reactivity of GA over time. The results may be relevant for the determination of GA concentration in various settings such as biocide analysis, hospital wastewaters, and others.
format Online
Article
Text
id pubmed-7246990
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72469902020-06-02 Stability of Glutaraldehyde in Biocide Compositions Matei, Alina Puscas, Cristina Patrascu, Iulia Lehene, Maria Ziebro, Julia Scurtu, Florina Baia, Monica Porumb, Dan Totos, Robert Silaghi-Dumitrescu, Radu Int J Mol Sci Article Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inherent to GA. An investigation of experimental and theoretical data is reported here, assessing the stability of GA solutions relevant for biocide compositions. GA solutions of various chemical composition and under varying circumstances were analyzed using spectroscopy (UV-VIS, Raman, NMR) coupled with density functional theory (DFT) calculations, as well as chemically, such as via the formation of imines in reaction/titration with glycine monitored at 270 nm; using LC-MS; or using SDS-PAGE analysis with GA as reagent in the polymerization of two test proteins- hemoglobin and myoglobin. The spectral properties of GA changed significantly over time, in a temperature-dependent manner; titration with glycine confirmed the spectral data. SDS-PAGE experiments demonstrated a non-linear and apparently unpredictable change in the reactivity of GA over time. The results may be relevant for the determination of GA concentration in various settings such as biocide analysis, hospital wastewaters, and others. MDPI 2020-05-10 /pmc/articles/PMC7246990/ /pubmed/32397667 http://dx.doi.org/10.3390/ijms21093372 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Matei, Alina
Puscas, Cristina
Patrascu, Iulia
Lehene, Maria
Ziebro, Julia
Scurtu, Florina
Baia, Monica
Porumb, Dan
Totos, Robert
Silaghi-Dumitrescu, Radu
Stability of Glutaraldehyde in Biocide Compositions
title Stability of Glutaraldehyde in Biocide Compositions
title_full Stability of Glutaraldehyde in Biocide Compositions
title_fullStr Stability of Glutaraldehyde in Biocide Compositions
title_full_unstemmed Stability of Glutaraldehyde in Biocide Compositions
title_short Stability of Glutaraldehyde in Biocide Compositions
title_sort stability of glutaraldehyde in biocide compositions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246990/
https://www.ncbi.nlm.nih.gov/pubmed/32397667
http://dx.doi.org/10.3390/ijms21093372
work_keys_str_mv AT mateialina stabilityofglutaraldehydeinbiocidecompositions
AT puscascristina stabilityofglutaraldehydeinbiocidecompositions
AT patrascuiulia stabilityofglutaraldehydeinbiocidecompositions
AT lehenemaria stabilityofglutaraldehydeinbiocidecompositions
AT ziebrojulia stabilityofglutaraldehydeinbiocidecompositions
AT scurtuflorina stabilityofglutaraldehydeinbiocidecompositions
AT baiamonica stabilityofglutaraldehydeinbiocidecompositions
AT porumbdan stabilityofglutaraldehydeinbiocidecompositions
AT totosrobert stabilityofglutaraldehydeinbiocidecompositions
AT silaghidumitrescuradu stabilityofglutaraldehydeinbiocidecompositions