Cargando…
Stability of Glutaraldehyde in Biocide Compositions
Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inhere...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246990/ https://www.ncbi.nlm.nih.gov/pubmed/32397667 http://dx.doi.org/10.3390/ijms21093372 |
_version_ | 1783538075543011328 |
---|---|
author | Matei, Alina Puscas, Cristina Patrascu, Iulia Lehene, Maria Ziebro, Julia Scurtu, Florina Baia, Monica Porumb, Dan Totos, Robert Silaghi-Dumitrescu, Radu |
author_facet | Matei, Alina Puscas, Cristina Patrascu, Iulia Lehene, Maria Ziebro, Julia Scurtu, Florina Baia, Monica Porumb, Dan Totos, Robert Silaghi-Dumitrescu, Radu |
author_sort | Matei, Alina |
collection | PubMed |
description | Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inherent to GA. An investigation of experimental and theoretical data is reported here, assessing the stability of GA solutions relevant for biocide compositions. GA solutions of various chemical composition and under varying circumstances were analyzed using spectroscopy (UV-VIS, Raman, NMR) coupled with density functional theory (DFT) calculations, as well as chemically, such as via the formation of imines in reaction/titration with glycine monitored at 270 nm; using LC-MS; or using SDS-PAGE analysis with GA as reagent in the polymerization of two test proteins- hemoglobin and myoglobin. The spectral properties of GA changed significantly over time, in a temperature-dependent manner; titration with glycine confirmed the spectral data. SDS-PAGE experiments demonstrated a non-linear and apparently unpredictable change in the reactivity of GA over time. The results may be relevant for the determination of GA concentration in various settings such as biocide analysis, hospital wastewaters, and others. |
format | Online Article Text |
id | pubmed-7246990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72469902020-06-02 Stability of Glutaraldehyde in Biocide Compositions Matei, Alina Puscas, Cristina Patrascu, Iulia Lehene, Maria Ziebro, Julia Scurtu, Florina Baia, Monica Porumb, Dan Totos, Robert Silaghi-Dumitrescu, Radu Int J Mol Sci Article Glutaraldehyde (GA) is used as biocide in hospitals. Recent public investigations on the chemical composition of biocides used in Romania have in some cases found GA, as a key ingredient, to be apparently diluted. However, these data did not explicitly consider the complex chemical equilibria inherent to GA. An investigation of experimental and theoretical data is reported here, assessing the stability of GA solutions relevant for biocide compositions. GA solutions of various chemical composition and under varying circumstances were analyzed using spectroscopy (UV-VIS, Raman, NMR) coupled with density functional theory (DFT) calculations, as well as chemically, such as via the formation of imines in reaction/titration with glycine monitored at 270 nm; using LC-MS; or using SDS-PAGE analysis with GA as reagent in the polymerization of two test proteins- hemoglobin and myoglobin. The spectral properties of GA changed significantly over time, in a temperature-dependent manner; titration with glycine confirmed the spectral data. SDS-PAGE experiments demonstrated a non-linear and apparently unpredictable change in the reactivity of GA over time. The results may be relevant for the determination of GA concentration in various settings such as biocide analysis, hospital wastewaters, and others. MDPI 2020-05-10 /pmc/articles/PMC7246990/ /pubmed/32397667 http://dx.doi.org/10.3390/ijms21093372 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Matei, Alina Puscas, Cristina Patrascu, Iulia Lehene, Maria Ziebro, Julia Scurtu, Florina Baia, Monica Porumb, Dan Totos, Robert Silaghi-Dumitrescu, Radu Stability of Glutaraldehyde in Biocide Compositions |
title | Stability of Glutaraldehyde in Biocide Compositions |
title_full | Stability of Glutaraldehyde in Biocide Compositions |
title_fullStr | Stability of Glutaraldehyde in Biocide Compositions |
title_full_unstemmed | Stability of Glutaraldehyde in Biocide Compositions |
title_short | Stability of Glutaraldehyde in Biocide Compositions |
title_sort | stability of glutaraldehyde in biocide compositions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7246990/ https://www.ncbi.nlm.nih.gov/pubmed/32397667 http://dx.doi.org/10.3390/ijms21093372 |
work_keys_str_mv | AT mateialina stabilityofglutaraldehydeinbiocidecompositions AT puscascristina stabilityofglutaraldehydeinbiocidecompositions AT patrascuiulia stabilityofglutaraldehydeinbiocidecompositions AT lehenemaria stabilityofglutaraldehydeinbiocidecompositions AT ziebrojulia stabilityofglutaraldehydeinbiocidecompositions AT scurtuflorina stabilityofglutaraldehydeinbiocidecompositions AT baiamonica stabilityofglutaraldehydeinbiocidecompositions AT porumbdan stabilityofglutaraldehydeinbiocidecompositions AT totosrobert stabilityofglutaraldehydeinbiocidecompositions AT silaghidumitrescuradu stabilityofglutaraldehydeinbiocidecompositions |