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Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley
BACKGROUND: Urogenital schistosomiasis, caused by infection with Schistosoma haematobium, is endemic in Niger but complicated by the presence of Schistosoma bovis, Schistosoma curassoni and S. haematobium group hybrids along with various Bulinus snail intermediate host species. Establishing the schi...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247258/ https://www.ncbi.nlm.nih.gov/pubmed/32448268 http://dx.doi.org/10.1186/s13071-020-04136-9 |
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author | Pennance, Tom Allan, Fiona Emery, Aidan Rabone, Muriel Cable, Jo Garba, Amadou Djirmay Hamidou, Amina Amadou Webster, Joanne P. Rollinson, David Webster, Bonnie L. |
author_facet | Pennance, Tom Allan, Fiona Emery, Aidan Rabone, Muriel Cable, Jo Garba, Amadou Djirmay Hamidou, Amina Amadou Webster, Joanne P. Rollinson, David Webster, Bonnie L. |
author_sort | Pennance, Tom |
collection | PubMed |
description | BACKGROUND: Urogenital schistosomiasis, caused by infection with Schistosoma haematobium, is endemic in Niger but complicated by the presence of Schistosoma bovis, Schistosoma curassoni and S. haematobium group hybrids along with various Bulinus snail intermediate host species. Establishing the schistosomes and snails involved in transmission aids disease surveillance whilst providing insights into snail-schistosome interactions/compatibilities and biology. METHODS: Infected Bulinus spp. were collected from 16 villages north and south of the Niamey region, Niger, between 2011 and 2015. From each Bulinus spp., 20–52 cercariae shed were analysed using microsatellite markers and a subset identified using the mitochondrial (mt) cox1 and nuclear ITS1 + 2 and 18S DNA regions. Infected Bulinus spp. were identified using both morphological and molecular analysis (partial mt cox1 region). RESULTS: A total of 87 infected Bulinus from 24 sites were found, 29 were molecularly confirmed as B. truncatus, three as B. forskalii and four as B. globosus. The remaining samples were morphologically identified as B. truncatus (n = 49) and B. forskalii (n = 2). The microsatellite analysis of 1124 cercariae revealed 186 cercarial multilocus genotypes (MLGs). Identical cercarial genotypes were frequently (60%) identified from the same snail (clonal populations from a single miracidia); however, several (40%) of the snails had cercariae of different genotypes (2–10 MLG’s) indicating multiple miracidial infections. Fifty-seven of the B. truncatus and all of the B. forskalii and B. globosus were shedding the Bovid schistosome S. bovis. The other B. truncatus were shedding the human schistosomes, S. haematobium (n = 6) and the S. haematobium group hybrids (n = 13). Two B. truncatus had co-infections with S. haematobium and S. haematobium group hybrids whilst no co-infections with S. bovis were observed. CONCLUSIONS: This study has advanced our understanding of human and bovid schistosomiasis transmission in the Niger River Valley region. Human Schistosoma species/forms (S. haematobium and S. haematobium hybrids) were found transmitted only in five villages whereas those causing veterinary schistosomiasis (S. bovis), were found in most villages. Bulinus truncatus was most abundant, transmitting all Schistosoma species, while the less abundant B. forskalii and B. globosus, only transmitted S. bovis. Our data suggest that species-specific biological traits may exist in relation to co-infections, snail-schistosome compatibility and intramolluscan schistosome development. [Image: see text] |
format | Online Article Text |
id | pubmed-7247258 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-72472582020-06-01 Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley Pennance, Tom Allan, Fiona Emery, Aidan Rabone, Muriel Cable, Jo Garba, Amadou Djirmay Hamidou, Amina Amadou Webster, Joanne P. Rollinson, David Webster, Bonnie L. Parasit Vectors Research BACKGROUND: Urogenital schistosomiasis, caused by infection with Schistosoma haematobium, is endemic in Niger but complicated by the presence of Schistosoma bovis, Schistosoma curassoni and S. haematobium group hybrids along with various Bulinus snail intermediate host species. Establishing the schistosomes and snails involved in transmission aids disease surveillance whilst providing insights into snail-schistosome interactions/compatibilities and biology. METHODS: Infected Bulinus spp. were collected from 16 villages north and south of the Niamey region, Niger, between 2011 and 2015. From each Bulinus spp., 20–52 cercariae shed were analysed using microsatellite markers and a subset identified using the mitochondrial (mt) cox1 and nuclear ITS1 + 2 and 18S DNA regions. Infected Bulinus spp. were identified using both morphological and molecular analysis (partial mt cox1 region). RESULTS: A total of 87 infected Bulinus from 24 sites were found, 29 were molecularly confirmed as B. truncatus, three as B. forskalii and four as B. globosus. The remaining samples were morphologically identified as B. truncatus (n = 49) and B. forskalii (n = 2). The microsatellite analysis of 1124 cercariae revealed 186 cercarial multilocus genotypes (MLGs). Identical cercarial genotypes were frequently (60%) identified from the same snail (clonal populations from a single miracidia); however, several (40%) of the snails had cercariae of different genotypes (2–10 MLG’s) indicating multiple miracidial infections. Fifty-seven of the B. truncatus and all of the B. forskalii and B. globosus were shedding the Bovid schistosome S. bovis. The other B. truncatus were shedding the human schistosomes, S. haematobium (n = 6) and the S. haematobium group hybrids (n = 13). Two B. truncatus had co-infections with S. haematobium and S. haematobium group hybrids whilst no co-infections with S. bovis were observed. CONCLUSIONS: This study has advanced our understanding of human and bovid schistosomiasis transmission in the Niger River Valley region. Human Schistosoma species/forms (S. haematobium and S. haematobium hybrids) were found transmitted only in five villages whereas those causing veterinary schistosomiasis (S. bovis), were found in most villages. Bulinus truncatus was most abundant, transmitting all Schistosoma species, while the less abundant B. forskalii and B. globosus, only transmitted S. bovis. Our data suggest that species-specific biological traits may exist in relation to co-infections, snail-schistosome compatibility and intramolluscan schistosome development. [Image: see text] BioMed Central 2020-05-24 /pmc/articles/PMC7247258/ /pubmed/32448268 http://dx.doi.org/10.1186/s13071-020-04136-9 Text en © The Author(s) 2020 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Pennance, Tom Allan, Fiona Emery, Aidan Rabone, Muriel Cable, Jo Garba, Amadou Djirmay Hamidou, Amina Amadou Webster, Joanne P. Rollinson, David Webster, Bonnie L. Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title | Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title_full | Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title_fullStr | Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title_full_unstemmed | Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title_short | Interactions between Schistosoma haematobium group species and their Bulinus spp. intermediate hosts along the Niger River Valley |
title_sort | interactions between schistosoma haematobium group species and their bulinus spp. intermediate hosts along the niger river valley |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247258/ https://www.ncbi.nlm.nih.gov/pubmed/32448268 http://dx.doi.org/10.1186/s13071-020-04136-9 |
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