Cargando…

MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway

MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Huadong, He, Haorong, Shen, Xiaoxu, Tang, Shuyue, Zhao, Jing, Cao, Xinao, Han, Shunshun, Cui, Can, Chen, Yuqi, Wei, Yuanhang, Wang, Yan, Li, Diyan, Zhu, Qing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247338/
https://www.ncbi.nlm.nih.gov/pubmed/32380777
http://dx.doi.org/10.3390/ijms21093274
_version_ 1783538134879830016
author Yin, Huadong
He, Haorong
Shen, Xiaoxu
Tang, Shuyue
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Wang, Yan
Li, Diyan
Zhu, Qing
author_facet Yin, Huadong
He, Haorong
Shen, Xiaoxu
Tang, Shuyue
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Wang, Yan
Li, Diyan
Zhu, Qing
author_sort Yin, Huadong
collection PubMed
description MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway.
format Online
Article
Text
id pubmed-7247338
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-72473382020-06-10 MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway Yin, Huadong He, Haorong Shen, Xiaoxu Tang, Shuyue Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Wang, Yan Li, Diyan Zhu, Qing Int J Mol Sci Article MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway. MDPI 2020-05-05 /pmc/articles/PMC7247338/ /pubmed/32380777 http://dx.doi.org/10.3390/ijms21093274 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yin, Huadong
He, Haorong
Shen, Xiaoxu
Tang, Shuyue
Zhao, Jing
Cao, Xinao
Han, Shunshun
Cui, Can
Chen, Yuqi
Wei, Yuanhang
Wang, Yan
Li, Diyan
Zhu, Qing
MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title_full MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title_fullStr MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title_full_unstemmed MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title_short MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
title_sort microrna profiling reveals an abundant mir-200a-3p promotes skeletal muscle satellite cell development by targeting tgf-β2 and regulating the tgf-β2/smad signaling pathway
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247338/
https://www.ncbi.nlm.nih.gov/pubmed/32380777
http://dx.doi.org/10.3390/ijms21093274
work_keys_str_mv AT yinhuadong micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT hehaorong micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT shenxiaoxu micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT tangshuyue micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT zhaojing micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT caoxinao micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT hanshunshun micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT cuican micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT chenyuqi micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT weiyuanhang micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT wangyan micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT lidiyan micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway
AT zhuqing micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway