Cargando…
MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway
MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247338/ https://www.ncbi.nlm.nih.gov/pubmed/32380777 http://dx.doi.org/10.3390/ijms21093274 |
_version_ | 1783538134879830016 |
---|---|
author | Yin, Huadong He, Haorong Shen, Xiaoxu Tang, Shuyue Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Wang, Yan Li, Diyan Zhu, Qing |
author_facet | Yin, Huadong He, Haorong Shen, Xiaoxu Tang, Shuyue Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Wang, Yan Li, Diyan Zhu, Qing |
author_sort | Yin, Huadong |
collection | PubMed |
description | MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway. |
format | Online Article Text |
id | pubmed-7247338 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72473382020-06-10 MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway Yin, Huadong He, Haorong Shen, Xiaoxu Tang, Shuyue Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Wang, Yan Li, Diyan Zhu, Qing Int J Mol Sci Article MicroRNAs (miRNAs) are evolutionarily conserved, small noncoding RNAs that play critical post-transcriptional regulatory roles in skeletal muscle development. Chicken is an optimal model to study skeletal muscle formation because its developmental anatomy is similar to that of mammals. In this study, we identified potential miRNAs in the breast muscle of broilers and layers at embryonic day 10 (E10), E13, E16, and E19. We detected 1836 miRNAs, 233 of which were differentially expressed between broilers and layers. In particular, miRNA-200a-3p was significantly more highly expressed in broilers than layers at three time points. In vitro experiments showed that miR-200a-3p accelerated differentiation and proliferation of chicken skeletal muscle satellite cells (SMSCs) and inhibited SMSCs apoptosis. The transforming growth factor 2 (TGF-β2) was identified as a target gene of miR-200a-3p, and which turned out to inhibit differentiation and proliferation, and promote apoptosis of SMSCs. Exogenous TGF-β2 increased the abundances of phosphorylated SMAD2 and SMAD3 proteins, and a miR-200a-3p mimic weakened this effect. The TGF-β2 inhibitor treatment reduced the promotional and inhibitory effects of miR-200a-3p on SMSC differentiation and apoptosis, respectively. Our results indicate that miRNAs are abundantly expressed during embryonic skeletal muscle development, and that miR-200a-3p promotes SMSC development by targeting TGF-β2 and regulating the TGF-β2/SMAD signaling pathway. MDPI 2020-05-05 /pmc/articles/PMC7247338/ /pubmed/32380777 http://dx.doi.org/10.3390/ijms21093274 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yin, Huadong He, Haorong Shen, Xiaoxu Tang, Shuyue Zhao, Jing Cao, Xinao Han, Shunshun Cui, Can Chen, Yuqi Wei, Yuanhang Wang, Yan Li, Diyan Zhu, Qing MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title | MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title_full | MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title_fullStr | MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title_full_unstemmed | MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title_short | MicroRNA Profiling Reveals an Abundant miR-200a-3p Promotes Skeletal Muscle Satellite Cell Development by Targeting TGF-β2 and Regulating the TGF-β2/SMAD Signaling Pathway |
title_sort | microrna profiling reveals an abundant mir-200a-3p promotes skeletal muscle satellite cell development by targeting tgf-β2 and regulating the tgf-β2/smad signaling pathway |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247338/ https://www.ncbi.nlm.nih.gov/pubmed/32380777 http://dx.doi.org/10.3390/ijms21093274 |
work_keys_str_mv | AT yinhuadong micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT hehaorong micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT shenxiaoxu micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT tangshuyue micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT zhaojing micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT caoxinao micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT hanshunshun micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT cuican micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT chenyuqi micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT weiyuanhang micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT wangyan micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT lidiyan micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway AT zhuqing micrornaprofilingrevealsanabundantmir200a3ppromotesskeletalmusclesatellitecelldevelopmentbytargetingtgfb2andregulatingthetgfb2smadsignalingpathway |