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Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD

Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due...

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Autores principales: Cataldi, Samuela, Borrelli, Antonella, Ceccarini, Maria Rachele, Nakashidze, Irina, Codini, Michela, Belov, Oleg, Ivanov, Alexander, Krasavin, Eugene, Ferri, Ivana, Conte, Carmela, Patria, Federica Filomena, Beccari, Tommaso, Mancini, Aldo, Curcio, Francesco, Ambesi-Impiombato, Francesco Saverio, Albi, Elisabetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247354/
https://www.ncbi.nlm.nih.gov/pubmed/32384654
http://dx.doi.org/10.3390/ijms21093281
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author Cataldi, Samuela
Borrelli, Antonella
Ceccarini, Maria Rachele
Nakashidze, Irina
Codini, Michela
Belov, Oleg
Ivanov, Alexander
Krasavin, Eugene
Ferri, Ivana
Conte, Carmela
Patria, Federica Filomena
Beccari, Tommaso
Mancini, Aldo
Curcio, Francesco
Ambesi-Impiombato, Francesco Saverio
Albi, Elisabetta
author_facet Cataldi, Samuela
Borrelli, Antonella
Ceccarini, Maria Rachele
Nakashidze, Irina
Codini, Michela
Belov, Oleg
Ivanov, Alexander
Krasavin, Eugene
Ferri, Ivana
Conte, Carmela
Patria, Federica Filomena
Beccari, Tommaso
Mancini, Aldo
Curcio, Francesco
Ambesi-Impiombato, Francesco Saverio
Albi, Elisabetta
author_sort Cataldi, Samuela
collection PubMed
description Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase.
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spelling pubmed-72473542020-06-10 Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta Int J Mol Sci Article Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase. MDPI 2020-05-06 /pmc/articles/PMC7247354/ /pubmed/32384654 http://dx.doi.org/10.3390/ijms21093281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cataldi, Samuela
Borrelli, Antonella
Ceccarini, Maria Rachele
Nakashidze, Irina
Codini, Michela
Belov, Oleg
Ivanov, Alexander
Krasavin, Eugene
Ferri, Ivana
Conte, Carmela
Patria, Federica Filomena
Beccari, Tommaso
Mancini, Aldo
Curcio, Francesco
Ambesi-Impiombato, Francesco Saverio
Albi, Elisabetta
Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title_full Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title_fullStr Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title_full_unstemmed Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title_short Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
title_sort acid and neutral sphingomyelinase behavior in radiation-induced liver pyroptosis and in the protective/preventive role of rmnsod
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247354/
https://www.ncbi.nlm.nih.gov/pubmed/32384654
http://dx.doi.org/10.3390/ijms21093281
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