Cargando…
Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD
Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247354/ https://www.ncbi.nlm.nih.gov/pubmed/32384654 http://dx.doi.org/10.3390/ijms21093281 |
_version_ | 1783538138610663424 |
---|---|
author | Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta |
author_facet | Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta |
author_sort | Cataldi, Samuela |
collection | PubMed |
description | Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase. |
format | Online Article Text |
id | pubmed-7247354 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-72473542020-06-10 Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta Int J Mol Sci Article Sphingomyelins (SMs) are a class of relevant bioactive molecules that act as key modulators of different cellular processes, such as growth arrest, exosome formation, and the inflammatory response influenced by many environmental conditions, leading to pyroptosis, a form of programmed cell death due to Caspase-1 involvement. To study liver pyroptosis and hepatic SM metabolism via both lysosomal acid SMase (aSMase) and endoplasmic reticulum/nucleus neutral SMase (nSMase) during the exposure of mice to radiation and to ascertain if this process can be modulated by protective molecules, we used an experimental design (previously used by us) to evaluate the effects of both ionizing radiation and a specific protective molecule (rMnSOD) in the brain in collaboration with the Joint Institute for Nuclear Research, Dubna (Russia). As shown by the Caspase-1 immunostaining of the liver sections, the radiation resulted in the loss of the normal cell structure alongside a progressive and dose-dependent increase of the labelling, treatment, and pretreatment with rMnSOD, which had a significant protective effect on the livers. SM metabolic analyses, performed on aSMase and nSMase gene expression, as well as protein content and activity, proved that rMnSOD was able to significantly reduce radiation-induced damage by playing both a protective role via aSMase and a preventive role via nSMase. MDPI 2020-05-06 /pmc/articles/PMC7247354/ /pubmed/32384654 http://dx.doi.org/10.3390/ijms21093281 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Cataldi, Samuela Borrelli, Antonella Ceccarini, Maria Rachele Nakashidze, Irina Codini, Michela Belov, Oleg Ivanov, Alexander Krasavin, Eugene Ferri, Ivana Conte, Carmela Patria, Federica Filomena Beccari, Tommaso Mancini, Aldo Curcio, Francesco Ambesi-Impiombato, Francesco Saverio Albi, Elisabetta Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title_full | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title_fullStr | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title_full_unstemmed | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title_short | Acid and Neutral Sphingomyelinase Behavior in Radiation-Induced Liver Pyroptosis and in the Protective/Preventive Role of rMnSOD |
title_sort | acid and neutral sphingomyelinase behavior in radiation-induced liver pyroptosis and in the protective/preventive role of rmnsod |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247354/ https://www.ncbi.nlm.nih.gov/pubmed/32384654 http://dx.doi.org/10.3390/ijms21093281 |
work_keys_str_mv | AT cataldisamuela acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT borrelliantonella acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT ceccarinimariarachele acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT nakashidzeirina acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT codinimichela acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT belovoleg acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT ivanovalexander acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT krasavineugene acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT ferriivana acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT contecarmela acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT patriafedericafilomena acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT beccaritommaso acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT mancinialdo acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT curciofrancesco acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT ambesiimpiombatofrancescosaverio acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod AT albielisabetta acidandneutralsphingomyelinasebehaviorinradiationinducedliverpyroptosisandintheprotectivepreventiveroleofrmnsod |