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Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates

2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to tradi...

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Autores principales: Nikolaivits, Efstratios, Agrafiotis, Andreas, Baira, Eirini, Le Goff, Géraldine, Tsafantakis, Nikolaos, Chavanich, Suchana A., Benayahu, Yehuda, Ouazzani, Jamal, Fokialakis, Nikolas, Topakas, Evangelos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247547/
https://www.ncbi.nlm.nih.gov/pubmed/32392868
http://dx.doi.org/10.3390/ijms21093317
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author Nikolaivits, Efstratios
Agrafiotis, Andreas
Baira, Eirini
Le Goff, Géraldine
Tsafantakis, Nikolaos
Chavanich, Suchana A.
Benayahu, Yehuda
Ouazzani, Jamal
Fokialakis, Nikolas
Topakas, Evangelos
author_facet Nikolaivits, Efstratios
Agrafiotis, Andreas
Baira, Eirini
Le Goff, Géraldine
Tsafantakis, Nikolaos
Chavanich, Suchana A.
Benayahu, Yehuda
Ouazzani, Jamal
Fokialakis, Nikolas
Topakas, Evangelos
author_sort Nikolaivits, Efstratios
collection PubMed
description 2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics.
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spelling pubmed-72475472020-06-10 Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates Nikolaivits, Efstratios Agrafiotis, Andreas Baira, Eirini Le Goff, Géraldine Tsafantakis, Nikolaos Chavanich, Suchana A. Benayahu, Yehuda Ouazzani, Jamal Fokialakis, Nikolas Topakas, Evangelos Int J Mol Sci Article 2,4-Dichlorophenol (2,4-DCP) is a ubiquitous environmental pollutant categorized as a priority pollutant by the United States (US) Environmental Protection Agency, posing adverse health effects on humans and wildlife. Bioremediation is proposed as an eco-friendly, cost-effective alternative to traditional physicochemical remediation techniques. In the present study, fungal strains were isolated from marine invertebrates and tested for their ability to biotransform 2,4-DCP at a concentration of 1 mM. The most competent strains were studied further for the expression of catechol dioxygenase activities and the produced metabolites. One strain, identified as Tritirachium sp., expressed high levels of extracellular catechol 1,2-dioxygenase activity. The same strain also produced a dechlorinated cleavage product of the starting compound, indicating the assimilation of the xenobiotic by the fungus. This work also enriches the knowledge about the mechanisms employed by marine-derived fungi in order to defend themselves against chlorinated xenobiotics. MDPI 2020-05-07 /pmc/articles/PMC7247547/ /pubmed/32392868 http://dx.doi.org/10.3390/ijms21093317 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nikolaivits, Efstratios
Agrafiotis, Andreas
Baira, Eirini
Le Goff, Géraldine
Tsafantakis, Nikolaos
Chavanich, Suchana A.
Benayahu, Yehuda
Ouazzani, Jamal
Fokialakis, Nikolas
Topakas, Evangelos
Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title_full Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title_fullStr Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title_full_unstemmed Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title_short Degradation Mechanism of 2,4-Dichlorophenol by Fungi Isolated from Marine Invertebrates
title_sort degradation mechanism of 2,4-dichlorophenol by fungi isolated from marine invertebrates
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247547/
https://www.ncbi.nlm.nih.gov/pubmed/32392868
http://dx.doi.org/10.3390/ijms21093317
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