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Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning

Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However,...

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Autores principales: Giricz, Zoltán, Makkos, András, Schreckenberg, Rolf, Pöling, Jochen, Lörchner, Holger, Kiss, Krisztina, Bencsik, Péter, Braun, Thomas, Schulz, Rainer, Ferdinandy, Péter, Schlüter, Klaus-Dieter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247556/
https://www.ncbi.nlm.nih.gov/pubmed/32397496
http://dx.doi.org/10.3390/ijms21093359
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author Giricz, Zoltán
Makkos, András
Schreckenberg, Rolf
Pöling, Jochen
Lörchner, Holger
Kiss, Krisztina
Bencsik, Péter
Braun, Thomas
Schulz, Rainer
Ferdinandy, Péter
Schlüter, Klaus-Dieter
author_facet Giricz, Zoltán
Makkos, András
Schreckenberg, Rolf
Pöling, Jochen
Lörchner, Holger
Kiss, Krisztina
Bencsik, Péter
Braun, Thomas
Schulz, Rainer
Ferdinandy, Péter
Schlüter, Klaus-Dieter
author_sort Giricz, Zoltán
collection PubMed
description Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in cardiac tissue and its regulation in vivo has not yet been addressed. The expression of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning. In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days after ischemia/reperfusion but this induction was abrogated by conditioning. As in cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p were associated with the regulation pattern of both molecules. Moreover, induction of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In summary, our data show that up-regulation of swiprosin-1 appears in the postischemic heart during cardiac remodeling and repair in different species.
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spelling pubmed-72475562020-06-10 Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning Giricz, Zoltán Makkos, András Schreckenberg, Rolf Pöling, Jochen Lörchner, Holger Kiss, Krisztina Bencsik, Péter Braun, Thomas Schulz, Rainer Ferdinandy, Péter Schlüter, Klaus-Dieter Int J Mol Sci Article Swiprosin-1 (EFhD2) is a molecule that triggers structural adaptation of isolated adult rat cardiomyocytes to cell culture conditions by initiating a process known as cell spreading. This process mimics central aspects of cardiac remodeling, as it occurs subsequent to myocardial infarction. However, expression of swiprosin-1 in cardiac tissue and its regulation in vivo has not yet been addressed. The expression of swiprosin-1 was analyzed in mice, rat, and pig hearts undergoing myocardial infarction or ischemia/reperfusion with or without cardiac protection by ischemic pre- and postconditioning. In mouse hearts, swiprosin-1 protein expression was increased after 4 and 7 days in myocardial infarct areas specifically in cardiomyocytes as verified by immunoblotting and histology. In rat hearts, swiprosin-1 mRNA expression was induced within 7 days after ischemia/reperfusion but this induction was abrogated by conditioning. As in cultured cardiomyocytes, the expression of swiprosin-1 was associated with a coinduction of arrestin-2, suggesting a common mechanism of regulation. Rno-miR-32-3p and rno-miR-34c-3p were associated with the regulation pattern of both molecules. Moreover, induction of swiprosin-1 and ssc-miR-34c was also confirmed in the infarct zone of pigs. In summary, our data show that up-regulation of swiprosin-1 appears in the postischemic heart during cardiac remodeling and repair in different species. MDPI 2020-05-09 /pmc/articles/PMC7247556/ /pubmed/32397496 http://dx.doi.org/10.3390/ijms21093359 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Giricz, Zoltán
Makkos, András
Schreckenberg, Rolf
Pöling, Jochen
Lörchner, Holger
Kiss, Krisztina
Bencsik, Péter
Braun, Thomas
Schulz, Rainer
Ferdinandy, Péter
Schlüter, Klaus-Dieter
Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title_full Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title_fullStr Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title_full_unstemmed Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title_short Swiprosin-1/EFhD-2 Expression in Cardiac Remodeling and Post-Infarct Repair: Effect of Ischemic Conditioning
title_sort swiprosin-1/efhd-2 expression in cardiac remodeling and post-infarct repair: effect of ischemic conditioning
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247556/
https://www.ncbi.nlm.nih.gov/pubmed/32397496
http://dx.doi.org/10.3390/ijms21093359
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