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Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints

The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, an...

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Autores principales: Ražná, Katarína, Sawinska, Zuzanna, Ivanišová, Eva, Vukovic, Nenad, Terentjeva, Margarita, Stričík, Michal, Kowalczewski, Przemysław Łukasz, Hlavačková, Lucia, Rovná, Katarína, Žiarovská, Jana, Kačániová, Miroslava
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247675/
https://www.ncbi.nlm.nih.gov/pubmed/32349345
http://dx.doi.org/10.3390/ijms21093087
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author Ražná, Katarína
Sawinska, Zuzanna
Ivanišová, Eva
Vukovic, Nenad
Terentjeva, Margarita
Stričík, Michal
Kowalczewski, Przemysław Łukasz
Hlavačková, Lucia
Rovná, Katarína
Žiarovská, Jana
Kačániová, Miroslava
author_facet Ražná, Katarína
Sawinska, Zuzanna
Ivanišová, Eva
Vukovic, Nenad
Terentjeva, Margarita
Stričík, Michal
Kowalczewski, Przemysław Łukasz
Hlavačková, Lucia
Rovná, Katarína
Žiarovská, Jana
Kačániová, Miroslava
author_sort Ražná, Katarína
collection PubMed
description The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, and this specificity was statistically significant. Ginkgo biloba L. from the locality of Košice had the best activity determined by the free radical scavenging activity (DPPH) (1.545 mg Trolox equivalent antioxidant capacity (TEAC)/g fresh matter (FM)) as well as the molybdenum-reducing antioxidant power (35.485 mg TEAC/g FM) methods. The highest content of total polyphenols (2.803 mg gallic acid equivalent (GAE)/g FM) and flavonoids (4.649 μg quercetin equivalent (QE)/g FM) was also detected in this sample. All samples of G. biloba leaf extracts showed significant antimicrobial activity against one or more of the examined bacterial species, and Staphylococcus aureus subsp. aureus CCM 2461 was found to be the most susceptible (minimal inhibition concentration MIC50 and MIC90 values of 64.2 and 72.2 µg/mL, respectively). Based on the results it was concluded that Ginkgo biloba L. extracts can be used as antimicrobial and antioxidant additives. Selected miRNA-based molecular markers were used to examine the environmental adaptability of Ginkgo biloba L. An almost-complete genotype clustering pattern based on locality was determined in the analysis that involved a species-specific gb-miR5261 marker. Morphologically specific exemplar, cv. Ohatsuki, was excluded.
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spelling pubmed-72476752020-06-10 Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints Ražná, Katarína Sawinska, Zuzanna Ivanišová, Eva Vukovic, Nenad Terentjeva, Margarita Stričík, Michal Kowalczewski, Przemysław Łukasz Hlavačková, Lucia Rovná, Katarína Žiarovská, Jana Kačániová, Miroslava Int J Mol Sci Article The aim of this study was to characterize extracts from the leaves of Ginkgo biloba L. from selected Slovakian localities in terms of the content of bioactive constituents, antioxidants and their antimicrobial properties. The results indicated that the content of antioxidants was sample-specific, and this specificity was statistically significant. Ginkgo biloba L. from the locality of Košice had the best activity determined by the free radical scavenging activity (DPPH) (1.545 mg Trolox equivalent antioxidant capacity (TEAC)/g fresh matter (FM)) as well as the molybdenum-reducing antioxidant power (35.485 mg TEAC/g FM) methods. The highest content of total polyphenols (2.803 mg gallic acid equivalent (GAE)/g FM) and flavonoids (4.649 μg quercetin equivalent (QE)/g FM) was also detected in this sample. All samples of G. biloba leaf extracts showed significant antimicrobial activity against one or more of the examined bacterial species, and Staphylococcus aureus subsp. aureus CCM 2461 was found to be the most susceptible (minimal inhibition concentration MIC50 and MIC90 values of 64.2 and 72.2 µg/mL, respectively). Based on the results it was concluded that Ginkgo biloba L. extracts can be used as antimicrobial and antioxidant additives. Selected miRNA-based molecular markers were used to examine the environmental adaptability of Ginkgo biloba L. An almost-complete genotype clustering pattern based on locality was determined in the analysis that involved a species-specific gb-miR5261 marker. Morphologically specific exemplar, cv. Ohatsuki, was excluded. MDPI 2020-04-27 /pmc/articles/PMC7247675/ /pubmed/32349345 http://dx.doi.org/10.3390/ijms21093087 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ražná, Katarína
Sawinska, Zuzanna
Ivanišová, Eva
Vukovic, Nenad
Terentjeva, Margarita
Stričík, Michal
Kowalczewski, Przemysław Łukasz
Hlavačková, Lucia
Rovná, Katarína
Žiarovská, Jana
Kačániová, Miroslava
Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title_full Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title_fullStr Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title_full_unstemmed Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title_short Properties of Ginkgo biloba L.: Antioxidant Characterization, Antimicrobial Activities, and Genomic MicroRNA Based Marker Fingerprints
title_sort properties of ginkgo biloba l.: antioxidant characterization, antimicrobial activities, and genomic microrna based marker fingerprints
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247675/
https://www.ncbi.nlm.nih.gov/pubmed/32349345
http://dx.doi.org/10.3390/ijms21093087
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