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Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice

Bacopa monnieri L. Wettst. (BM) is a botanical component of Ayurvedic medicines and of dietary supplements used worldwide for cognitive health and function. We previously reported that administration of BM alcoholic extract (BME) prevents trimethyltin (TMT)-induced cognitive deficits and hippocampal...

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Autores principales: Pham, Hang Thi Nguyet, Tran, Hong Nguyen, Nguyen, Phuong Thi, Le, Xoan Thi, Nguyen, Khoi Minh, Phan, Sinh Viet, Yoneyama, Masanori, Ogita, Kiyokazu, Yamaguchi, Taro, Folk, William R., Yamaguchi, Masamitsu, Matsumoto, Kinzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247711/
https://www.ncbi.nlm.nih.gov/pubmed/32397562
http://dx.doi.org/10.3390/ijms21093365
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author Pham, Hang Thi Nguyet
Tran, Hong Nguyen
Nguyen, Phuong Thi
Le, Xoan Thi
Nguyen, Khoi Minh
Phan, Sinh Viet
Yoneyama, Masanori
Ogita, Kiyokazu
Yamaguchi, Taro
Folk, William R.
Yamaguchi, Masamitsu
Matsumoto, Kinzo
author_facet Pham, Hang Thi Nguyet
Tran, Hong Nguyen
Nguyen, Phuong Thi
Le, Xoan Thi
Nguyen, Khoi Minh
Phan, Sinh Viet
Yoneyama, Masanori
Ogita, Kiyokazu
Yamaguchi, Taro
Folk, William R.
Yamaguchi, Masamitsu
Matsumoto, Kinzo
author_sort Pham, Hang Thi Nguyet
collection PubMed
description Bacopa monnieri L. Wettst. (BM) is a botanical component of Ayurvedic medicines and of dietary supplements used worldwide for cognitive health and function. We previously reported that administration of BM alcoholic extract (BME) prevents trimethyltin (TMT)-induced cognitive deficits and hippocampal cell damage and promotes TMT-induced hippocampal neurogenesis. In this study, we demonstrate that administration of BME improves spatial working memory in adolescent (5-week- old) healthy mice but not adult (8-week-old) mice. Moreover, improved spatial working memory was retained even at 4 weeks after terminating 1-week treatment of adolescent mice. One-week BME treatment of adolescent mice significantly enhanced hippocampal BrdU incorporation and expression of genes involved in neurogenesis determined by RNAseq analysis. Cell death, as detected by histochemistry, appeared not to be significant. A significant increase in neurogenesis was observed in the dentate gyrus region 4 weeks after terminating 1-week treatment of adolescent mice with BME. Bacopaside I, an active component of BME, promoted the proliferation of neural progenitor cells in vitro in a concentration-dependent manner via the facilitation of the Akt and ERK1/2 signaling. These results suggest that BME enhances spatial working memory in healthy adolescent mice by promoting hippocampal neurogenesis and that the effects of BME are due, in significant amounts, to bacopaside I.
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spelling pubmed-72477112020-06-10 Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice Pham, Hang Thi Nguyet Tran, Hong Nguyen Nguyen, Phuong Thi Le, Xoan Thi Nguyen, Khoi Minh Phan, Sinh Viet Yoneyama, Masanori Ogita, Kiyokazu Yamaguchi, Taro Folk, William R. Yamaguchi, Masamitsu Matsumoto, Kinzo Int J Mol Sci Article Bacopa monnieri L. Wettst. (BM) is a botanical component of Ayurvedic medicines and of dietary supplements used worldwide for cognitive health and function. We previously reported that administration of BM alcoholic extract (BME) prevents trimethyltin (TMT)-induced cognitive deficits and hippocampal cell damage and promotes TMT-induced hippocampal neurogenesis. In this study, we demonstrate that administration of BME improves spatial working memory in adolescent (5-week- old) healthy mice but not adult (8-week-old) mice. Moreover, improved spatial working memory was retained even at 4 weeks after terminating 1-week treatment of adolescent mice. One-week BME treatment of adolescent mice significantly enhanced hippocampal BrdU incorporation and expression of genes involved in neurogenesis determined by RNAseq analysis. Cell death, as detected by histochemistry, appeared not to be significant. A significant increase in neurogenesis was observed in the dentate gyrus region 4 weeks after terminating 1-week treatment of adolescent mice with BME. Bacopaside I, an active component of BME, promoted the proliferation of neural progenitor cells in vitro in a concentration-dependent manner via the facilitation of the Akt and ERK1/2 signaling. These results suggest that BME enhances spatial working memory in healthy adolescent mice by promoting hippocampal neurogenesis and that the effects of BME are due, in significant amounts, to bacopaside I. MDPI 2020-05-09 /pmc/articles/PMC7247711/ /pubmed/32397562 http://dx.doi.org/10.3390/ijms21093365 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pham, Hang Thi Nguyet
Tran, Hong Nguyen
Nguyen, Phuong Thi
Le, Xoan Thi
Nguyen, Khoi Minh
Phan, Sinh Viet
Yoneyama, Masanori
Ogita, Kiyokazu
Yamaguchi, Taro
Folk, William R.
Yamaguchi, Masamitsu
Matsumoto, Kinzo
Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title_full Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title_fullStr Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title_full_unstemmed Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title_short Bacopa monnieri (L.) Wettst. Extract Improves Memory Performance via Promotion of Neurogenesis in the Hippocampal Dentate Gyrus of Adolescent Mice
title_sort bacopa monnieri (l.) wettst. extract improves memory performance via promotion of neurogenesis in the hippocampal dentate gyrus of adolescent mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247711/
https://www.ncbi.nlm.nih.gov/pubmed/32397562
http://dx.doi.org/10.3390/ijms21093365
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