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Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community

The effects of nodulation properties of legumes on the rhizosphere bacterial community are still not clear. To determine the effects of nodulation phenotypes on bacterial communities in the rhizosphere of soybean plants, we performed high-throughput sequencing of the 16S rRNA gene to estimate the rh...

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Autores principales: Wang, Hao, Gu, Chuntao, Liu, Xiaofeng, Yang, Chunwei, Li, Wenbin, Wang, Shaodong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247815/
https://www.ncbi.nlm.nih.gov/pubmed/32528420
http://dx.doi.org/10.3389/fmicb.2020.00750
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author Wang, Hao
Gu, Chuntao
Liu, Xiaofeng
Yang, Chunwei
Li, Wenbin
Wang, Shaodong
author_facet Wang, Hao
Gu, Chuntao
Liu, Xiaofeng
Yang, Chunwei
Li, Wenbin
Wang, Shaodong
author_sort Wang, Hao
collection PubMed
description The effects of nodulation properties of legumes on the rhizosphere bacterial community are still not clear. To determine the effects of nodulation phenotypes on bacterial communities in the rhizosphere of soybean plants, we performed high-throughput sequencing of the 16S rRNA gene to estimate the rhizosphere bacterial community of three soybean lines with different nodulation phenotypes grown in soil supplied with different levels of N fertilizer. The results revealed that both the soybean nodulation phenotypes and the N levels affected the rhizosphere bacteria community, but the nodulation phenotypes contributed more than the N-supply. The diversity of bacteria was decreased in the rhizosphere of super-nodulating phenotype. The response of rhizosphere bacterial communities to the soil available nitrogen (AN) concentrations was different than the response with the three nodulation phenotypes of soybean which was more stable in the wild-type (Nod(+)) soybean samples than that in the mutant samples (Nod(–) and Nod(++)). Bradyrhizobium in the rhizosphere was positively correlated with nodule number and negatively correlated to AN in the soil, while Burkholderia and Dyella were positively correlated with nodule biomass and nitrogenase activity. These results demonstrated that the nodulation phenotype of soybean affects the rhizosphere microbiome.
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spelling pubmed-72478152020-06-10 Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community Wang, Hao Gu, Chuntao Liu, Xiaofeng Yang, Chunwei Li, Wenbin Wang, Shaodong Front Microbiol Microbiology The effects of nodulation properties of legumes on the rhizosphere bacterial community are still not clear. To determine the effects of nodulation phenotypes on bacterial communities in the rhizosphere of soybean plants, we performed high-throughput sequencing of the 16S rRNA gene to estimate the rhizosphere bacterial community of three soybean lines with different nodulation phenotypes grown in soil supplied with different levels of N fertilizer. The results revealed that both the soybean nodulation phenotypes and the N levels affected the rhizosphere bacteria community, but the nodulation phenotypes contributed more than the N-supply. The diversity of bacteria was decreased in the rhizosphere of super-nodulating phenotype. The response of rhizosphere bacterial communities to the soil available nitrogen (AN) concentrations was different than the response with the three nodulation phenotypes of soybean which was more stable in the wild-type (Nod(+)) soybean samples than that in the mutant samples (Nod(–) and Nod(++)). Bradyrhizobium in the rhizosphere was positively correlated with nodule number and negatively correlated to AN in the soil, while Burkholderia and Dyella were positively correlated with nodule biomass and nitrogenase activity. These results demonstrated that the nodulation phenotype of soybean affects the rhizosphere microbiome. Frontiers Media S.A. 2020-05-12 /pmc/articles/PMC7247815/ /pubmed/32528420 http://dx.doi.org/10.3389/fmicb.2020.00750 Text en Copyright © 2020 Wang, Gu, Liu, Yang, Li and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Hao
Gu, Chuntao
Liu, Xiaofeng
Yang, Chunwei
Li, Wenbin
Wang, Shaodong
Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title_full Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title_fullStr Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title_full_unstemmed Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title_short Impact of Soybean Nodulation Phenotypes and Nitrogen Fertilizer Levels on the Rhizosphere Bacterial Community
title_sort impact of soybean nodulation phenotypes and nitrogen fertilizer levels on the rhizosphere bacterial community
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247815/
https://www.ncbi.nlm.nih.gov/pubmed/32528420
http://dx.doi.org/10.3389/fmicb.2020.00750
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