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Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission

HIV-1 infection is transmitted primarily by sexual exposure, with semen being the principal contaminated fluid. However, HIV-specific immune response in semen has been understudied. We investigated specific parameters of the innate, cellular, and humoral immune response that may affect semen infecti...

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Autores principales: Suphaphiphat, Karunasinee, Bernard-Stoecklin, Sibylle, Gommet, Céline, Delache, Benoit, Dereuddre-Bosquet, Nathalie, Kent, Stephen J., Wines, Bruce D., Hogarth, P. Mark, Le Grand, Roger, Cavarelli, Mariangela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247827/
https://www.ncbi.nlm.nih.gov/pubmed/32528466
http://dx.doi.org/10.3389/fimmu.2020.00850
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author Suphaphiphat, Karunasinee
Bernard-Stoecklin, Sibylle
Gommet, Céline
Delache, Benoit
Dereuddre-Bosquet, Nathalie
Kent, Stephen J.
Wines, Bruce D.
Hogarth, P. Mark
Le Grand, Roger
Cavarelli, Mariangela
author_facet Suphaphiphat, Karunasinee
Bernard-Stoecklin, Sibylle
Gommet, Céline
Delache, Benoit
Dereuddre-Bosquet, Nathalie
Kent, Stephen J.
Wines, Bruce D.
Hogarth, P. Mark
Le Grand, Roger
Cavarelli, Mariangela
author_sort Suphaphiphat, Karunasinee
collection PubMed
description HIV-1 infection is transmitted primarily by sexual exposure, with semen being the principal contaminated fluid. However, HIV-specific immune response in semen has been understudied. We investigated specific parameters of the innate, cellular, and humoral immune response that may affect semen infectivity in macaques infected with SIVmac251. Serial semen levels of cytokines and chemokines, SIV-specific antibodies, neutralization, and FcγR-mediated functions and SIV-specific T-cell responses were assessed and compared to systemic responses across 53 cynomolgus macaques. SIV infection induced an overall inflammatory state in the semen. Several pro-inflammatory molecules correlated with SIV virus levels. Effector CD8(+) T cells were expanded in semen upon infection. SIV-specific CD8(+) T-cells that expressed multiple effector molecules (IFN-γ(+)MIP-1β(+)TNF(+/−)) were induced in the semen of a subset of SIV-infected macaques, but this did not correlate with local viral control. SIV-specific IgG, commonly capable of engaging the FcγRIIIa receptor, was detected in most semen samples although this positively correlated with seminal viral load. Several inflammatory immune responses in semen develop in the context of higher levels of SIV seminal plasma viremia. These inflammatory immune responses could play a role in viral transmission and should be considered in the development of preventive and prophylactic vaccines.
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spelling pubmed-72478272020-06-10 Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission Suphaphiphat, Karunasinee Bernard-Stoecklin, Sibylle Gommet, Céline Delache, Benoit Dereuddre-Bosquet, Nathalie Kent, Stephen J. Wines, Bruce D. Hogarth, P. Mark Le Grand, Roger Cavarelli, Mariangela Front Immunol Immunology HIV-1 infection is transmitted primarily by sexual exposure, with semen being the principal contaminated fluid. However, HIV-specific immune response in semen has been understudied. We investigated specific parameters of the innate, cellular, and humoral immune response that may affect semen infectivity in macaques infected with SIVmac251. Serial semen levels of cytokines and chemokines, SIV-specific antibodies, neutralization, and FcγR-mediated functions and SIV-specific T-cell responses were assessed and compared to systemic responses across 53 cynomolgus macaques. SIV infection induced an overall inflammatory state in the semen. Several pro-inflammatory molecules correlated with SIV virus levels. Effector CD8(+) T cells were expanded in semen upon infection. SIV-specific CD8(+) T-cells that expressed multiple effector molecules (IFN-γ(+)MIP-1β(+)TNF(+/−)) were induced in the semen of a subset of SIV-infected macaques, but this did not correlate with local viral control. SIV-specific IgG, commonly capable of engaging the FcγRIIIa receptor, was detected in most semen samples although this positively correlated with seminal viral load. Several inflammatory immune responses in semen develop in the context of higher levels of SIV seminal plasma viremia. These inflammatory immune responses could play a role in viral transmission and should be considered in the development of preventive and prophylactic vaccines. Frontiers Media S.A. 2020-05-12 /pmc/articles/PMC7247827/ /pubmed/32528466 http://dx.doi.org/10.3389/fimmu.2020.00850 Text en Copyright © 2020 Suphaphiphat, Bernard-Stoecklin, Gommet, Delache, Dereuddre-Bosquet, Kent, Wines, Hogarth, Le Grand and Cavarelli. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Suphaphiphat, Karunasinee
Bernard-Stoecklin, Sibylle
Gommet, Céline
Delache, Benoit
Dereuddre-Bosquet, Nathalie
Kent, Stephen J.
Wines, Bruce D.
Hogarth, P. Mark
Le Grand, Roger
Cavarelli, Mariangela
Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title_full Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title_fullStr Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title_full_unstemmed Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title_short Innate and Adaptive Anti-SIV Responses in Macaque Semen: Implications for Infectivity and Risk of Transmission
title_sort innate and adaptive anti-siv responses in macaque semen: implications for infectivity and risk of transmission
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7247827/
https://www.ncbi.nlm.nih.gov/pubmed/32528466
http://dx.doi.org/10.3389/fimmu.2020.00850
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