Cargando…
Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis
Pain evoked by visceral inflammation is often ‘referred’ to the somatic level. Transient receptor potential ankyrin 1 (TRPA1) has been reported to contribute to visceral pain-like behavior in dextran sulfate sodium (DSS)-evoked colitis. However, the role of TRPA1 in somatic component of hypersensiti...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248095/ https://www.ncbi.nlm.nih.gov/pubmed/32451393 http://dx.doi.org/10.1038/s41598-020-65618-5 |
_version_ | 1783538294978510848 |
---|---|
author | Jain, Piyush Materazzi, Serena De Logu, Francesco Rossi Degl’Innocenti, Duccio Fusi, Camilla Li Puma, Simone Marone, Ilaria M. Coppi, Elisabetta Holzer, Peter Geppetti, Pierangelo Nassini, Romina |
author_facet | Jain, Piyush Materazzi, Serena De Logu, Francesco Rossi Degl’Innocenti, Duccio Fusi, Camilla Li Puma, Simone Marone, Ilaria M. Coppi, Elisabetta Holzer, Peter Geppetti, Pierangelo Nassini, Romina |
author_sort | Jain, Piyush |
collection | PubMed |
description | Pain evoked by visceral inflammation is often ‘referred’ to the somatic level. Transient receptor potential ankyrin 1 (TRPA1) has been reported to contribute to visceral pain-like behavior in dextran sulfate sodium (DSS)-evoked colitis. However, the role of TRPA1 in somatic component of hypersensitivity due to visceral inflammation is unknown. The present study investigated the role of TRPA1 in colitis-evoked mechanical hypersensitivity at the somatic level. Colitis was induced in mice by adding DSS to drinking water for one week. Control and DSS-treated mice were tested for various parameters of colitis as well as mechanical pain sensitivity in abdominal and facial regions. DSS treatment caused mechanical hypersensitivity in the abdominal and facial skin. Pharmacological blockade or genetic deletion of TRPA1 prevented the colitis-associated mechanical hypersensitivity in the abdominal and facial skin areas although the severity of colitis remained unaltered. DSS treatment increased expression of TRPA1 mRNA in cultured dorsal root ganglion (DRG) neurons, but not trigeminal ganglion neurons, and selectively enhanced currents evoked by the TRPA1 agonist, allyl isothiocyanate, in cultured DRG neurons. Our findings indicate that the TRPA1 channel contributes to colitis-associated mechanical hypersensitivity in somatic tissues, an effect associated with upregulation of TRPA1 expression and responsiveness in DRG nociceptors. |
format | Online Article Text |
id | pubmed-7248095 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-72480952020-06-04 Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis Jain, Piyush Materazzi, Serena De Logu, Francesco Rossi Degl’Innocenti, Duccio Fusi, Camilla Li Puma, Simone Marone, Ilaria M. Coppi, Elisabetta Holzer, Peter Geppetti, Pierangelo Nassini, Romina Sci Rep Article Pain evoked by visceral inflammation is often ‘referred’ to the somatic level. Transient receptor potential ankyrin 1 (TRPA1) has been reported to contribute to visceral pain-like behavior in dextran sulfate sodium (DSS)-evoked colitis. However, the role of TRPA1 in somatic component of hypersensitivity due to visceral inflammation is unknown. The present study investigated the role of TRPA1 in colitis-evoked mechanical hypersensitivity at the somatic level. Colitis was induced in mice by adding DSS to drinking water for one week. Control and DSS-treated mice were tested for various parameters of colitis as well as mechanical pain sensitivity in abdominal and facial regions. DSS treatment caused mechanical hypersensitivity in the abdominal and facial skin. Pharmacological blockade or genetic deletion of TRPA1 prevented the colitis-associated mechanical hypersensitivity in the abdominal and facial skin areas although the severity of colitis remained unaltered. DSS treatment increased expression of TRPA1 mRNA in cultured dorsal root ganglion (DRG) neurons, but not trigeminal ganglion neurons, and selectively enhanced currents evoked by the TRPA1 agonist, allyl isothiocyanate, in cultured DRG neurons. Our findings indicate that the TRPA1 channel contributes to colitis-associated mechanical hypersensitivity in somatic tissues, an effect associated with upregulation of TRPA1 expression and responsiveness in DRG nociceptors. Nature Publishing Group UK 2020-05-25 /pmc/articles/PMC7248095/ /pubmed/32451393 http://dx.doi.org/10.1038/s41598-020-65618-5 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jain, Piyush Materazzi, Serena De Logu, Francesco Rossi Degl’Innocenti, Duccio Fusi, Camilla Li Puma, Simone Marone, Ilaria M. Coppi, Elisabetta Holzer, Peter Geppetti, Pierangelo Nassini, Romina Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title | Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title_full | Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title_fullStr | Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title_full_unstemmed | Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title_short | Transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
title_sort | transient receptor potential ankyrin 1 contributes to somatic pain hypersensitivity in experimental colitis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248095/ https://www.ncbi.nlm.nih.gov/pubmed/32451393 http://dx.doi.org/10.1038/s41598-020-65618-5 |
work_keys_str_mv | AT jainpiyush transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT materazziserena transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT delogufrancesco transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT rossideglinnocentiduccio transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT fusicamilla transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT lipumasimone transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT maroneilariam transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT coppielisabetta transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT holzerpeter transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT geppettipierangelo transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis AT nassiniromina transientreceptorpotentialankyrin1contributestosomaticpainhypersensitivityinexperimentalcolitis |