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Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections

Hendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treat...

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Autores principales: Chen, Mingyue, Tachedjian, Mary, Marsh, Glenn A., Cui, Jie, Wang, Lin-Fa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248276/
https://www.ncbi.nlm.nih.gov/pubmed/32508793
http://dx.doi.org/10.3389/fmicb.2020.00986
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author Chen, Mingyue
Tachedjian, Mary
Marsh, Glenn A.
Cui, Jie
Wang, Lin-Fa
author_facet Chen, Mingyue
Tachedjian, Mary
Marsh, Glenn A.
Cui, Jie
Wang, Lin-Fa
author_sort Chen, Mingyue
collection PubMed
description Hendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treatment methods for human are available. In contrast, previous experimental infection studies have suggested that CedV is non-pathogenic. Flying foxes (pteropid bats) in Australia are the natural reservoirs of both viruses, but the cellular responses of bats to these viral infections remain unclear. Here, we infected bat and human cells with these viruses. We then examined the total transcriptomic landscapes of the cells at 6 or 24 h post infection. Unexpectedly, despite the close phylogenetic relationship between HeV and CedV, there was a dramatic difference in cellular gene expression patterns in response to the two different infections. It is likely that minor differences in the phosphoprotein (P) gene coding strategy between the two viruses cause the observed incongruence in host transcriptomic divergence and viral lethality. This study greatly expands our understanding of the pathogenic mechanisms of henipaviruses.
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spelling pubmed-72482762020-06-05 Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections Chen, Mingyue Tachedjian, Mary Marsh, Glenn A. Cui, Jie Wang, Lin-Fa Front Microbiol Microbiology Hendra virus (HeV) and Cedar virus (CedV) are henipaviruses, which fall into the Paramyxoviridae family of single-stranded, negative-sense RNA viruses. HeV is classified as a Biosafety Level-4 (BSL-4) agent, as it is highly pathogenic and is often fatal to humans. To date, no HeV prevention or treatment methods for human are available. In contrast, previous experimental infection studies have suggested that CedV is non-pathogenic. Flying foxes (pteropid bats) in Australia are the natural reservoirs of both viruses, but the cellular responses of bats to these viral infections remain unclear. Here, we infected bat and human cells with these viruses. We then examined the total transcriptomic landscapes of the cells at 6 or 24 h post infection. Unexpectedly, despite the close phylogenetic relationship between HeV and CedV, there was a dramatic difference in cellular gene expression patterns in response to the two different infections. It is likely that minor differences in the phosphoprotein (P) gene coding strategy between the two viruses cause the observed incongruence in host transcriptomic divergence and viral lethality. This study greatly expands our understanding of the pathogenic mechanisms of henipaviruses. Frontiers Media S.A. 2020-05-19 /pmc/articles/PMC7248276/ /pubmed/32508793 http://dx.doi.org/10.3389/fmicb.2020.00986 Text en Copyright © 2020 Chen, Tachedjian, Marsh, Cui and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Chen, Mingyue
Tachedjian, Mary
Marsh, Glenn A.
Cui, Jie
Wang, Lin-Fa
Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title_full Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title_fullStr Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title_full_unstemmed Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title_short Distinct Cell Transcriptomic Landscapes Upon Henipavirus Infections
title_sort distinct cell transcriptomic landscapes upon henipavirus infections
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248276/
https://www.ncbi.nlm.nih.gov/pubmed/32508793
http://dx.doi.org/10.3389/fmicb.2020.00986
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