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Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer

Periostin (POSTN) is an extracellular matrix protein associated with tumor progression and shorter survival in prostate cancer (PCa). Here, we performed an integrative analysis of POSTN’s role in patients with PCa. Clinical and POSTN data from large-scale datasets were analyzed. POSTN cutoffs were i...

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Autores principales: Cattrini, Carlo, Barboro, Paola, Rubagotti, Alessandra, Zinoli, Linda, Zanardi, Elisa, Capaia, Matteo, Boccardo, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248449/
https://www.ncbi.nlm.nih.gov/pubmed/32416542
http://dx.doi.org/10.1016/j.tranon.2020.100789
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author Cattrini, Carlo
Barboro, Paola
Rubagotti, Alessandra
Zinoli, Linda
Zanardi, Elisa
Capaia, Matteo
Boccardo, Francesco
author_facet Cattrini, Carlo
Barboro, Paola
Rubagotti, Alessandra
Zinoli, Linda
Zanardi, Elisa
Capaia, Matteo
Boccardo, Francesco
author_sort Cattrini, Carlo
collection PubMed
description Periostin (POSTN) is an extracellular matrix protein associated with tumor progression and shorter survival in prostate cancer (PCa). Here, we performed an integrative analysis of POSTN’s role in patients with PCa. Clinical and POSTN data from large-scale datasets were analyzed. POSTN cutoffs were identified with X-Tile, and STRING was used for protein-protein interaction analysis. In a cohort of 48 patients with metastatic castration-resistant prostate cancer (mCRPC), we used the AdnaTest platform to isolate circulating tumor cells and extract POSTN mRNA. Plasma samples were also tested for POSTN protein expression by dot blot assay. Data from large-scale datasets did not reveal any association between POSTN genetic alterations and outcome. In primary tumors, we found a significant correlation between POSTN mRNA overexpression, worse baseline prognostic features, and shorter disease-free survival. POSTN was overexpressed in mCRPC and correlated with aggressive features. In our cohort of mCRPC patients, we found a positive correlation between POSTN plasma levels and androgen-receptor variant 7 positivity and an association with shorter overall survival. Our integrative analysis shows that POSTN is associated with poor clinical features and worse outcome in patients with PCa. Further studies are warranted to uncover the function of POSTN in PCa progression and to validate the prognostic significance of POSTN in mCRPC.
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spelling pubmed-72484492020-05-28 Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer Cattrini, Carlo Barboro, Paola Rubagotti, Alessandra Zinoli, Linda Zanardi, Elisa Capaia, Matteo Boccardo, Francesco Transl Oncol Original article Periostin (POSTN) is an extracellular matrix protein associated with tumor progression and shorter survival in prostate cancer (PCa). Here, we performed an integrative analysis of POSTN’s role in patients with PCa. Clinical and POSTN data from large-scale datasets were analyzed. POSTN cutoffs were identified with X-Tile, and STRING was used for protein-protein interaction analysis. In a cohort of 48 patients with metastatic castration-resistant prostate cancer (mCRPC), we used the AdnaTest platform to isolate circulating tumor cells and extract POSTN mRNA. Plasma samples were also tested for POSTN protein expression by dot blot assay. Data from large-scale datasets did not reveal any association between POSTN genetic alterations and outcome. In primary tumors, we found a significant correlation between POSTN mRNA overexpression, worse baseline prognostic features, and shorter disease-free survival. POSTN was overexpressed in mCRPC and correlated with aggressive features. In our cohort of mCRPC patients, we found a positive correlation between POSTN plasma levels and androgen-receptor variant 7 positivity and an association with shorter overall survival. Our integrative analysis shows that POSTN is associated with poor clinical features and worse outcome in patients with PCa. Further studies are warranted to uncover the function of POSTN in PCa progression and to validate the prognostic significance of POSTN in mCRPC. Neoplasia Press 2020-05-14 /pmc/articles/PMC7248449/ /pubmed/32416542 http://dx.doi.org/10.1016/j.tranon.2020.100789 Text en © 2020 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original article
Cattrini, Carlo
Barboro, Paola
Rubagotti, Alessandra
Zinoli, Linda
Zanardi, Elisa
Capaia, Matteo
Boccardo, Francesco
Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title_full Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title_fullStr Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title_full_unstemmed Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title_short Integrative Analysis of Periostin in Primary and Advanced Prostate Cancer
title_sort integrative analysis of periostin in primary and advanced prostate cancer
topic Original article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248449/
https://www.ncbi.nlm.nih.gov/pubmed/32416542
http://dx.doi.org/10.1016/j.tranon.2020.100789
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