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Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer
Recent studies have shown that microRNAs (miRs) play a key role in the regulation of cancer development. In the present study, reverse transcription-quantitative PCR was used to detect the expression of miR-1 in breast cancer and adjacent tissues, and survival analysis was performed to compare the l...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248535/ https://www.ncbi.nlm.nih.gov/pubmed/32377691 http://dx.doi.org/10.3892/mmr.2020.11111 |
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author | Peng, Jing Yuan, Chenwei Wu, Ziping Wang, Yan Yin, Wenjin Lin, Yanping Zhou, Liheng Lu, Jinsong |
author_facet | Peng, Jing Yuan, Chenwei Wu, Ziping Wang, Yan Yin, Wenjin Lin, Yanping Zhou, Liheng Lu, Jinsong |
author_sort | Peng, Jing |
collection | PubMed |
description | Recent studies have shown that microRNAs (miRs) play a key role in the regulation of cancer development. In the present study, reverse transcription-quantitative PCR was used to detect the expression of miR-1 in breast cancer and adjacent tissues, and survival analysis was performed to compare the low-expression groups with the Kaplan-Meier method. Overexpression of miR-1 was used to observe the effects on the proliferation, migration and invasion of breast cancer cells in vitro and in vivo. Moreover, Bcl-2 expression was measured by western blotting and luciferase assays after the overexpression of miR-1. The present study reported that miR-1 is expressed at low levels in breast cancer and that cell proliferation, migration and invasion are inhibited in miR-1-overexpressing cells. Enhanced miR-1 expression can also increase cell apoptosis. The present study also demonstrated that Bcl-2 is a potential target of miR-1. In vivo studies indicate that overexpression of miR-1 decreases tumor volume and weight in nude mice. The data from the present study demonstrated for the first time that overexpression of miR-1 increases the sensitivity of breast cancer cells to paclitaxel and cisplatin. The present study provided new evidence for the important role of miR-1 in the tumorigenesis and drug sensitivity of breast cancer. |
format | Online Article Text |
id | pubmed-7248535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-72485352020-05-27 Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer Peng, Jing Yuan, Chenwei Wu, Ziping Wang, Yan Yin, Wenjin Lin, Yanping Zhou, Liheng Lu, Jinsong Mol Med Rep Articles Recent studies have shown that microRNAs (miRs) play a key role in the regulation of cancer development. In the present study, reverse transcription-quantitative PCR was used to detect the expression of miR-1 in breast cancer and adjacent tissues, and survival analysis was performed to compare the low-expression groups with the Kaplan-Meier method. Overexpression of miR-1 was used to observe the effects on the proliferation, migration and invasion of breast cancer cells in vitro and in vivo. Moreover, Bcl-2 expression was measured by western blotting and luciferase assays after the overexpression of miR-1. The present study reported that miR-1 is expressed at low levels in breast cancer and that cell proliferation, migration and invasion are inhibited in miR-1-overexpressing cells. Enhanced miR-1 expression can also increase cell apoptosis. The present study also demonstrated that Bcl-2 is a potential target of miR-1. In vivo studies indicate that overexpression of miR-1 decreases tumor volume and weight in nude mice. The data from the present study demonstrated for the first time that overexpression of miR-1 increases the sensitivity of breast cancer cells to paclitaxel and cisplatin. The present study provided new evidence for the important role of miR-1 in the tumorigenesis and drug sensitivity of breast cancer. D.A. Spandidos 2020-07 2020-05-04 /pmc/articles/PMC7248535/ /pubmed/32377691 http://dx.doi.org/10.3892/mmr.2020.11111 Text en Copyright: © Peng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Peng, Jing Yuan, Chenwei Wu, Ziping Wang, Yan Yin, Wenjin Lin, Yanping Zhou, Liheng Lu, Jinsong Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title | Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title_full | Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title_fullStr | Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title_full_unstemmed | Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title_short | Upregulation of microRNA-1 inhibits proliferation and metastasis of breast cancer |
title_sort | upregulation of microrna-1 inhibits proliferation and metastasis of breast cancer |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248535/ https://www.ncbi.nlm.nih.gov/pubmed/32377691 http://dx.doi.org/10.3892/mmr.2020.11111 |
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