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Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine

Lumpy skin disease and Rift Valley fever are two high-priority livestock diseases which have the potential to spread into previously free regions through animal movement and/or vectors, as well as intentional release by bioterrorists. Since the distribution range of both diseases is similar in Afric...

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Autores principales: Wallace, David B., Mather, A., Kara, P. D., Naicker, Leeann, Mokoena, Nobalanda B., Pretorius, A., Nefefe, T., Thema, N., Babiuk, Shawn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248559/
https://www.ncbi.nlm.nih.gov/pubmed/32509806
http://dx.doi.org/10.3389/fvets.2020.00256
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author Wallace, David B.
Mather, A.
Kara, P. D.
Naicker, Leeann
Mokoena, Nobalanda B.
Pretorius, A.
Nefefe, T.
Thema, N.
Babiuk, Shawn
author_facet Wallace, David B.
Mather, A.
Kara, P. D.
Naicker, Leeann
Mokoena, Nobalanda B.
Pretorius, A.
Nefefe, T.
Thema, N.
Babiuk, Shawn
author_sort Wallace, David B.
collection PubMed
description Lumpy skin disease and Rift Valley fever are two high-priority livestock diseases which have the potential to spread into previously free regions through animal movement and/or vectors, as well as intentional release by bioterrorists. Since the distribution range of both diseases is similar in Africa, it makes sense to use a bivalent vaccine to control them. This may lead to the more consistent and sustainable use of vaccination against Rift Valley fever through a more cost-effective vaccine. In this study, a recombinant lumpy skin disease virus was constructed in which the thymidine kinase gene was used as the insertion site for the Gn and Gc protective glycoprotein genes of Rift Valley fever virus using homologous recombination. Selection markers, the enhanced green fluorescent protein and Escherichia coli guanidine phosphoribosyl transferase (gpt), were used for selection of recombinant virus and in a manner enabling a second recombination event to occur upon removal of the gpt selection-pressure allowing the removal of both marker genes in the final product. This recombinant virus, LSD-RVF.mf, was selected to homogeneity, characterized and evaluated in cattle as a vaccine to show protection against both lumpy skin disease and Rift Valley fever in cattle. The results demonstrate that the LSD-RVF.mf is safe, immunogenic and can protect cattle against both diseases.
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spelling pubmed-72485592020-06-05 Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine Wallace, David B. Mather, A. Kara, P. D. Naicker, Leeann Mokoena, Nobalanda B. Pretorius, A. Nefefe, T. Thema, N. Babiuk, Shawn Front Vet Sci Veterinary Science Lumpy skin disease and Rift Valley fever are two high-priority livestock diseases which have the potential to spread into previously free regions through animal movement and/or vectors, as well as intentional release by bioterrorists. Since the distribution range of both diseases is similar in Africa, it makes sense to use a bivalent vaccine to control them. This may lead to the more consistent and sustainable use of vaccination against Rift Valley fever through a more cost-effective vaccine. In this study, a recombinant lumpy skin disease virus was constructed in which the thymidine kinase gene was used as the insertion site for the Gn and Gc protective glycoprotein genes of Rift Valley fever virus using homologous recombination. Selection markers, the enhanced green fluorescent protein and Escherichia coli guanidine phosphoribosyl transferase (gpt), were used for selection of recombinant virus and in a manner enabling a second recombination event to occur upon removal of the gpt selection-pressure allowing the removal of both marker genes in the final product. This recombinant virus, LSD-RVF.mf, was selected to homogeneity, characterized and evaluated in cattle as a vaccine to show protection against both lumpy skin disease and Rift Valley fever in cattle. The results demonstrate that the LSD-RVF.mf is safe, immunogenic and can protect cattle against both diseases. Frontiers Media S.A. 2020-05-19 /pmc/articles/PMC7248559/ /pubmed/32509806 http://dx.doi.org/10.3389/fvets.2020.00256 Text en Copyright © 2020 Wallace, Mather, Kara, Naicker, Mokoena, Pretorius, Nefefe, Thema and Babiuk. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Wallace, David B.
Mather, A.
Kara, P. D.
Naicker, Leeann
Mokoena, Nobalanda B.
Pretorius, A.
Nefefe, T.
Thema, N.
Babiuk, Shawn
Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title_full Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title_fullStr Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title_full_unstemmed Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title_short Protection of Cattle Elicited Using a Bivalent Lumpy Skin Disease Virus-Vectored Recombinant Rift Valley Fever Vaccine
title_sort protection of cattle elicited using a bivalent lumpy skin disease virus-vectored recombinant rift valley fever vaccine
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248559/
https://www.ncbi.nlm.nih.gov/pubmed/32509806
http://dx.doi.org/10.3389/fvets.2020.00256
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