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Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells

Biological potential of plant extracts are widely described. Because their oral or topical administration is usually recommended, intestinal mucous and skin are the first surfaces exposed to such preparations. Therefore, we asked the question whether phenolic and non-polar fractions of the extracts...

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Autores principales: Sadowska, Beata, Rywaniak, Joanna, Cichocka, Anna, Cichocka, Kinga, Żuchowski, Jerzy, Wójcik-Bojek, Urszula, Więckowska-Szakiel, Marzena, Różalska, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248724/
https://www.ncbi.nlm.nih.gov/pubmed/32397559
http://dx.doi.org/10.3390/molecules25092238
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author Sadowska, Beata
Rywaniak, Joanna
Cichocka, Anna
Cichocka, Kinga
Żuchowski, Jerzy
Wójcik-Bojek, Urszula
Więckowska-Szakiel, Marzena
Różalska, Barbara
author_facet Sadowska, Beata
Rywaniak, Joanna
Cichocka, Anna
Cichocka, Kinga
Żuchowski, Jerzy
Wójcik-Bojek, Urszula
Więckowska-Szakiel, Marzena
Różalska, Barbara
author_sort Sadowska, Beata
collection PubMed
description Biological potential of plant extracts are widely described. Because their oral or topical administration is usually recommended, intestinal mucous and skin are the first surfaces exposed to such preparations. Therefore, we asked the question whether phenolic and non-polar fractions of the extracts from fruits, twigs, and leaves of sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) would be able to modulate the functions of human physiological barrier. The study was carried on caucasian colon epithelial-like Caco-2 cells and human foreskin fibroblasts HFF-1 line. Cell secretory activity (ELISA), the expression of cell surface molecules (flow cytometry), cell migration during wound healing in vitro (scratch assay) were assessed. It was demonstrated for the first time, that sea buckthorn extracts can improve intestinal and skin barrier by increasing of ICAM-1 expression on colon epithelial cells and intensification of IL-8 production by fibroblasts. On the other hand, an inhibition of fibroblasts migration in the presence of those preparations was noted. Therefore, greater attention should be paid on precise description of plant extracts effect depended on target cells and their role to give adequate recommendations for such preparations use.
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spelling pubmed-72487242020-08-13 Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells Sadowska, Beata Rywaniak, Joanna Cichocka, Anna Cichocka, Kinga Żuchowski, Jerzy Wójcik-Bojek, Urszula Więckowska-Szakiel, Marzena Różalska, Barbara Molecules Article Biological potential of plant extracts are widely described. Because their oral or topical administration is usually recommended, intestinal mucous and skin are the first surfaces exposed to such preparations. Therefore, we asked the question whether phenolic and non-polar fractions of the extracts from fruits, twigs, and leaves of sea buckthorn (Elaeagnus rhamnoides (L.) A. Nelson) would be able to modulate the functions of human physiological barrier. The study was carried on caucasian colon epithelial-like Caco-2 cells and human foreskin fibroblasts HFF-1 line. Cell secretory activity (ELISA), the expression of cell surface molecules (flow cytometry), cell migration during wound healing in vitro (scratch assay) were assessed. It was demonstrated for the first time, that sea buckthorn extracts can improve intestinal and skin barrier by increasing of ICAM-1 expression on colon epithelial cells and intensification of IL-8 production by fibroblasts. On the other hand, an inhibition of fibroblasts migration in the presence of those preparations was noted. Therefore, greater attention should be paid on precise description of plant extracts effect depended on target cells and their role to give adequate recommendations for such preparations use. MDPI 2020-05-09 /pmc/articles/PMC7248724/ /pubmed/32397559 http://dx.doi.org/10.3390/molecules25092238 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sadowska, Beata
Rywaniak, Joanna
Cichocka, Anna
Cichocka, Kinga
Żuchowski, Jerzy
Wójcik-Bojek, Urszula
Więckowska-Szakiel, Marzena
Różalska, Barbara
Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title_full Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title_fullStr Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title_full_unstemmed Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title_short Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells
title_sort phenolic and non-polar fractions of the extracts from fruits, leaves, and twigs of elaeagnus rhamnoides (l.) a. nelson—the implications for human barrier cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248724/
https://www.ncbi.nlm.nih.gov/pubmed/32397559
http://dx.doi.org/10.3390/molecules25092238
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