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A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics

Salmonella is a main cause of foodborne illnesses and rapid screening of Salmonella is the key to prevent Salmonella outbreaks, however available detection methods either require a long time, or need complex pretreatment, or have low sensitivity. In this study, a microfluidic biosensor was developed...

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Autores principales: Yao, Lan, Zheng, Lingyan, Cai, Gaozhe, Wang, Siyuan, Wang, Lei, Lin, Jianhan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248794/
https://www.ncbi.nlm.nih.gov/pubmed/32403342
http://dx.doi.org/10.3390/s20092738
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author Yao, Lan
Zheng, Lingyan
Cai, Gaozhe
Wang, Siyuan
Wang, Lei
Lin, Jianhan
author_facet Yao, Lan
Zheng, Lingyan
Cai, Gaozhe
Wang, Siyuan
Wang, Lei
Lin, Jianhan
author_sort Yao, Lan
collection PubMed
description Salmonella is a main cause of foodborne illnesses and rapid screening of Salmonella is the key to prevent Salmonella outbreaks, however available detection methods either require a long time, or need complex pretreatment, or have low sensitivity. In this study, a microfluidic biosensor was developed for Salmonella detection using viscoelastic inertial microfluidics for separating magnetic bacteria from unbound magnetic nanoparticles (MNPs) and enzyme catalytic colorimetry for amplifying biological signals. The polyclonal antibodies and horseradish peroxidase (HRP) modified MNPs were first used to specifically capture Salmonella to form magnetic HRP-bacteria. Both magnetic HRP-bacteria and unbound MNPs were magnetically separated from background and resuspended in viscoelastic polyvinylpyrrolidone solution as sample flow. When sample flow was injected with polyvinylpyrrolidone sheath flow into a T-shaped microchannel, larger-sized magnetic HRP-bacteria could penetrate the sample flow, however smaller-sized MNPs remained in the sample flow due to weaker inertial lift force and elastic lift force, resulting in continuous-flow separation of magnetic HRP-bacteria. Finally, magnetic HRP-bacteria were collected and concentrated to catalyze tetramethyl benzidine, and absorbance was measured to determine the bacteria. This biosensor was able to detect Salmonella as low as 30 CFU/mL in 1 h and featured the advantages of shorter time due to a one-step immunoreaction, easier extension due to only one antibody and one label, and lower cost due to less expensive materials.
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spelling pubmed-72487942020-06-10 A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics Yao, Lan Zheng, Lingyan Cai, Gaozhe Wang, Siyuan Wang, Lei Lin, Jianhan Sensors (Basel) Article Salmonella is a main cause of foodborne illnesses and rapid screening of Salmonella is the key to prevent Salmonella outbreaks, however available detection methods either require a long time, or need complex pretreatment, or have low sensitivity. In this study, a microfluidic biosensor was developed for Salmonella detection using viscoelastic inertial microfluidics for separating magnetic bacteria from unbound magnetic nanoparticles (MNPs) and enzyme catalytic colorimetry for amplifying biological signals. The polyclonal antibodies and horseradish peroxidase (HRP) modified MNPs were first used to specifically capture Salmonella to form magnetic HRP-bacteria. Both magnetic HRP-bacteria and unbound MNPs were magnetically separated from background and resuspended in viscoelastic polyvinylpyrrolidone solution as sample flow. When sample flow was injected with polyvinylpyrrolidone sheath flow into a T-shaped microchannel, larger-sized magnetic HRP-bacteria could penetrate the sample flow, however smaller-sized MNPs remained in the sample flow due to weaker inertial lift force and elastic lift force, resulting in continuous-flow separation of magnetic HRP-bacteria. Finally, magnetic HRP-bacteria were collected and concentrated to catalyze tetramethyl benzidine, and absorbance was measured to determine the bacteria. This biosensor was able to detect Salmonella as low as 30 CFU/mL in 1 h and featured the advantages of shorter time due to a one-step immunoreaction, easier extension due to only one antibody and one label, and lower cost due to less expensive materials. MDPI 2020-05-11 /pmc/articles/PMC7248794/ /pubmed/32403342 http://dx.doi.org/10.3390/s20092738 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yao, Lan
Zheng, Lingyan
Cai, Gaozhe
Wang, Siyuan
Wang, Lei
Lin, Jianhan
A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title_full A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title_fullStr A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title_full_unstemmed A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title_short A Rapid and Sensitive Salmonella Biosensor Based on Viscoelastic Inertial Microfluidics
title_sort rapid and sensitive salmonella biosensor based on viscoelastic inertial microfluidics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7248794/
https://www.ncbi.nlm.nih.gov/pubmed/32403342
http://dx.doi.org/10.3390/s20092738
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